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Diagnostic application of formalin fixed archived tissues for detection of foot-and-mouth disease

期刊: JOURNAL OF VIROLOGICAL METHODS, 2023; 318 ()

Early and definitive disease diagnosis is critical for effective disease control. 50% buffered glycerine is commonly used viral transport medium, whic......

Evaluation of extraction and enrichment methods for recovery of respiratory RNA viruses in a metagenomics approach

期刊: JOURNAL OF VIROLOGICAL METHODS, 2023; 314 ()

Viral metagenomics is increasingly applied in viral detection and virome characterization. Different extraction and enrichment techniques may be adopt......

Development of cell culture based peste des petits ruminants (PPR) virus vaccine candidate from Bangladeshi isolates

期刊: JOURNAL OF VIROLOGICAL METHODS, 2023; 314 ()

This study was conducted to develop a cell culture based PPR virus vaccine candidate using recent Bangladeshi strain of peste des petits ruminant's (P......

A new simple and effective method for PLRV infection to screen for virus resistance in potato

期刊: JOURNAL OF VIROLOGICAL METHODS, 2023; 315 ()

Effective screening of plant germplasm collections for resistance to plant viruses requires that there is a rapid and efficient system in place to cha......

Detection of cytomegalovirus in bronchoalveolar lavage fluid from immunocompromised patients with pneumonitis by viral culture and DNA quantification

期刊: JOURNAL OF VIROLOGICAL METHODS, 2023; 317 ()

Purpose: To compare the detection of human cytomegalovirus (HCMV) in bronchoalveolar lavage (BAL) fluid by viral culture and quantitative polymerase c......

Development and validation of a nanoplate-based digital PCR assay for absolute MPXV quantification

期刊: JOURNAL OF VIROLOGICAL METHODS, 2023; 321 ()

Quantification of mpox virus (MPXV) across different human body anatomical sites can provide insights about the most likely transmission routes, so me......

Development of recombinant West Nile virus expressing mCherry reporter protein

期刊: JOURNAL OF VIROLOGICAL METHODS, 2023; 317 ()

West Nile virus (WNV) is transmitted to humans and animals by a mosquito and enters the central nervous system, leading to lethal encephalitis. Report......

Pan-yellow fever virus detection and lineage assignment by real-time RT-PCR and amplicon sequencing

期刊: JOURNAL OF VIROLOGICAL METHODS, 2023; 316 ()

Yellow fever disease is a viral zoonosis that may result in a severe hemorrhagic disease. A safe and effective vaccine used in mass immunization campa......

Development of a real-time RT-PCR system applicable for rapid and pen-side diagnosis of foot-and-mouth disease using a portable device, PicoGene® PCR1100

期刊: JOURNAL OF VIROLOGICAL METHODS, 2023; 319 ()

Foot-and-mouth disease (FMD) is a highly contagious viral vesicular disease, causing devastating losses to the livestock industry. A diagnostic method......

Antigenicity of subregions of recombinant bovine leukemia virus (BLV) glycoprotein gp51 for antibody detection

期刊: JOURNAL OF VIROLOGICAL METHODS, 2023; 311 ()

Bovine leukemia virus (BLV) is an enveloped virus, found worldwide that can infect cattle and induce many subclinical symptoms and malignant tumors. B......

Inactivation of airborne SARS-CoV-2 by thyme volatile oil vapor phase

期刊: JOURNAL OF VIROLOGICAL METHODS, 2023; 312 ()

Background: The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) caused a global pandemic mainly through air transmission. Novel variants ......

A simple transwell-based infection system for obtaining pure populations of VZV-infected cells

期刊: JOURNAL OF VIROLOGICAL METHODS, 2023; 312 ()

Varicella-Zoster virus (VZV) is a human herpesvirus and causes chickenpox and shingles. Research into its molecular virology has been hampered by a la......

?CoviSwiftTM?: A point-of-care RT-PCR device for SARS-CoV-2 and its variant detection

期刊: JOURNAL OF VIROLOGICAL METHODS, 2023; 315 ()

We present the point-of-care (POC) molecular diagnostic solution, evaluated during COVID-19 pandemic caused by SARS-CoV-2 (Dec 2021). The POC comprise......

Development of an immunochromatographic strip test for antigen detection of elephant endotheliotropic herpesvirus in Asian elephants (Elephas maximus)

期刊: JOURNAL OF VIROLOGICAL METHODS, 2023; 311 ()

Elephant endotheliotropic herpesvirus (EEHV) is the causative agent of EEHV-hemorrhagic disease (EEHV-HD) in elephants worldwide. This disease is high......

Development of a high-throughput flow cytometric neutralization assay to screen for human enterovirus A71 (EVA71) neutralizing antibodies

期刊: JOURNAL OF VIROLOGICAL METHODS, 2023; 322 ()

The conventional method for screening neutralizing antibodies to human enterovirus A71 (EVA71) (microneutralization assay) is time consuming and requi......

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