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Blocking Abundant RNA Transcripts by High-Affinity Oligonucleotides during Transcriptome Library Preparation

期刊: BIOLOGICAL PROCEDURES ONLINE, 2023; 25 (1)

BackgroundRNA sequencing has become the gold standard for transcriptome analysis but has an inherent limitation of challenging quantification of low-a......

Protocol for Evaluating In Vivo the Activation of the P2RX7 Immunomodulator

期刊: BIOLOGICAL PROCEDURES ONLINE, 2023; 25 (1)

Background P2RX7 is a purinergic receptor with pleiotropic activities that is activated by high levels of extracellular ATP that are found in inflamed......

Isolation and characterization of apical papilla cells from root end of human third molar and their differentiation into cementoblast cells: an in vitro study

期刊: BIOLOGICAL PROCEDURES ONLINE, 2023; 25 (1)

BackgroundPeriodontal regeneration, treatment of periodontal-related diseases and improving the function of implants are global therapeutic challenges......

Comparison of two protocols for the generation of iPSC-derived human astrocytes

期刊: BIOLOGICAL PROCEDURES ONLINE, 2023; 25 (1)

BackgroundAstrocytes have recently gained attention as key contributors to the pathogenesis of neurodegenerative disorders including Parkinson's disea......

Screening of Natural Compounds for CYP11A1 Stimulation Against Cell Renal Cell Carcinoma

期刊: BIOLOGICAL PROCEDURES ONLINE, 2023; 25 (1)

BackgroundRenal cancer therapies are challenging owing to the extensive spreading of this cancer to other organs and its ability to pose resistance to......

Deinococcus radiodurans-derived membrane vesicles protect HaCaT cells against H2O2-induced oxidative stress via modulation of MAPK and Nrf2/ARE pathways

期刊: BIOLOGICAL PROCEDURES ONLINE, 2023; 25 (1)

Background Deinococcus radiodurans is a robust bacterium that can withstand harsh environments that cause oxidative stress to macromolecules due to it......

Integrating Omics and CRISPR Technology for Identification and Verification of Genomic Safe Harbor Loci in the Chicken Genome

期刊: BIOLOGICAL PROCEDURES ONLINE, 2023; 25 (1)

BackgroundOne of the most prominent questions in the field of transgenesis is 'Where in the genome to integrate a transgene?'. Escape from epigenetic ......

A high-throughput screening system for SARS-CoV-2 entry inhibition, syncytia formation and cell toxicity

期刊: BIOLOGICAL PROCEDURES ONLINE, 2023; 25 (1)

BackgroundThe entry of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) into the host cell is mediated through the binding of the SARS-CoV......

A 3D human co-culture to model neuron-astrocyte interactions in tauopathies

期刊: BIOLOGICAL PROCEDURES ONLINE, 2023; 25 (1)

BackgroundIntraneuronal tau aggregation is the major pathological hallmark of neurodegenerative tauopathies. It is now generally acknowledged that tau......

Visualisation of Bluetongue Virus in the Salivary Apparatus of Culicoides Biting Midges Highlights the Accessory Glands as a Primary Arboviral Infection Site

期刊: BIOLOGICAL PROCEDURES ONLINE, 2023; 25 (1)

Background Arthropods transmit a wide range of pathogens of importance for the global health of humans, animals, and plants. One group of these arthro......

Development of a Large-Scale Pathogen Screening Test for the Biosafety Evaluation of Canine Mesenchymal Stem Cells

期刊: BIOLOGICAL PROCEDURES ONLINE, 2023; 25 (1)

BackgroundThe action of mesenchymal stem cells (MSCs) is the subject of intense research in the field of regenerative medicine, including their potent......

A Novel Dual-Reporter System Reveals Distinct Characteristics of Exosome-Mediated Protein Secretion in Human Cells

期刊: BIOLOGICAL PROCEDURES ONLINE, 2023; 25 (1)

BackgroundExosomes, a special subtype of extracellular vesicles derived from human cells, serve as vital mediators of intercellular communication by t......

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