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Analytical separation of plasmid DNA isoforms using anion exchanging chromatographic monoliths with 6 μm channels

期刊: ELECTROPHORESIS, 2023; ()

High-performance liquid chromatography (HPLC)-based analytical assays are used to effectively monitor purity and quantity of plasmid DNA (pDNA) throug......

Preferential exclusion chromatography as a capture step for extracellular AAV harvest from adherent and suspension productions

期刊: ELECTROPHORESIS, 2023; ()

Preferential exclusion chromatography (PXC) sometimes described as hydrophobic interaction chromatography is a well-known, but not widely used techniq......

Concepts and recent advances in microchip electrophoresis coupled to mass spectrometry: Technologies and applications

期刊: ELECTROPHORESIS, 2023; 44 (1-2)

The online coupling of microchip electrophoresis (ME) as a fast, highly efficient, and low-cost miniaturized separation technique to mass spectrometry......

High-throughput capillary electrophoresis analysis of biopharmaceuticals utilizing sequential injections

期刊: ELECTROPHORESIS, 2023; 44 (9-10)

The complexity of biotherapeutic products implies an ever-increasing list of product quality attributes that need to be monitored and characterized. I......

Speeding up SDS-PAGE: Theory and experiment

期刊: ELECTROPHORESIS, 2023; 44 (15-16)

In order to accelerate Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), here we propose an optimized version of the technique ena......

Determination of acidity constants of pyridines, imidazoles, and oximes by capillary electrophoresis

期刊: ELECTROPHORESIS, 2023; 44 (17-18)

The acidity constant in the form of pK(a) is one of the most important physicochemical quantities. There are prediction tools available for calculatin......

Comprehensive assessment of 12 commercial DNA-binding dyes as alternatives to ethidium bromide for agarose gel electrophoresis

期刊: ELECTROPHORESIS, 2023; ()

Staining and visualization of the nucleic acid bands on agarose gels using ethidium bromide (EB) has been a widely used technique in molecular biology......

Optimization of rAAV capture step purification using SO3 monolith chromatography

期刊: ELECTROPHORESIS, 2023; ()

Adeno-associated virus (AAV) vectors are crucial tools for gene therapy applications. As AAVs are administered in vivo, stringent purity requirements ......

Capillary electrophoresis with capacitively coupled contactless conductivity detection (C4D) for rapid and simple determination of lactate in sweat

期刊: ELECTROPHORESIS, 2023; ()

An analytical method based on capillary electrophoresis (CE) using capacitively coupled contactless conductivity detection (C4D) was developed and val......

Low-cost, high-throughput and rapid-prototyped 3D-integrated dielectrophoretic channels for continuous cell enrichment and separation

期刊: ELECTROPHORESIS, 2023; 44 (11-12)

Microfluidic devices for dielectrophoretic cell separation are typically designed and constructed using microfabrication methods in a clean room, requ......

Electrophoresis of a dielectric droplet with constant surface charge density

期刊: ELECTROPHORESIS, 2023; 44 (23)

Electrophoresis of a dielectric fluid droplet with constant surface charge density is investigated theoretically in this study. A pseudo-spectral meth......

Scalable multimodal weak anion exchange chromatographic purification for stable mRNA drug substance

期刊: ELECTROPHORESIS, 2023; ()

Messenger RNA (mRNA) has emerged as a modality with immense therapeutic potential. Recent innovations in production process of mRNA call for procedure......

Surface sampling capillary electrophoresis-mass spectrometry for a direct chemical characterization of tissue and blood samples

期刊: ELECTROPHORESIS, 2023; 44 (3-4)

Capillary electrophoresis (CE) is a powerful separation tool for non-targeted analysis of chemically complex samples, such as blood, urine, and tissue......

Enantioselective CE-MS analysis of ketamine metabolites in urine

期刊: ELECTROPHORESIS, 2023; 44 (1-2)

The chiral drug ketamine has long-lasting antidepressant effects with a fast onset and is also suitable to treat patients with therapy-resistant depre......

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