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An optimized method for the induction and purification of mouse bone marrow dendritic cells

期刊: JOURNAL OF IMMUNOLOGICAL METHODS, 2021; 495 ()

Dendritic cells (DCs) play an essential role in the initiation of adaptive immune responses, but they are rare in all organs. The traditional methods ......

Construction of a full-length antibody phage display vector

期刊: JOURNAL OF IMMUNOLOGICAL METHODS, 2021; 494 ()

Antibody phage display technology plays an important role in the development of monoclonal antibodies, humanization, and affinity evolution of antibod......

Development of a sensitive and stable chemiluminescent immunoassay for detection of birch pollen allergic specific IgE based on recombinant Bet v1 protein

期刊: JOURNAL OF IMMUNOLOGICAL METHODS, 2021; 493 ()

Background Quantitative detection of allergens is of great significance for clarifying the cause, treatment, and prevention of allergy disease. Birch ......

Establishment of a homogeneous immunoassay-light-initiated chemiluminescence assay for detecting anti-Mullerian hormone in human serum

期刊: JOURNAL OF IMMUNOLOGICAL METHODS, 2021; 494 ()

Anti-Mullerian hormone (AMH) is known as a reliable marker of ovarian reserve (OR). The determination of AMH is of great importance and most existed A......

Quantification of trivalent non-replicating rotavirus vaccine antigens in the presence of aluminum adjuvant

期刊: JOURNAL OF IMMUNOLOGICAL METHODS, 2021; 494 ()

Parenterally administered rotavirus vaccines may overcome the low efficacy observed in resource-poor regions that use live oral formulations. We have ......

A novel antigen immunochromatography fluorometric strip for rapid detection and application of pathogenic bacterial high-quality antibody

期刊: JOURNAL OF IMMUNOLOGICAL METHODS, 2021; 494 ()

Unlike traditional immunoassay strips, a novel antigen immunechromatography fluorometric strip (AICFS) using inactivated bacterial antigen instead of ......

Gene knockout in highly purified mouse hematopoietic stem cells by CRISPR/Cas9 technology

期刊: JOURNAL OF IMMUNOLOGICAL METHODS, 2021; 495 ()

The CRISPR/Cas9 system has been used for genome editing of human and mouse cells. In this study, we established a protocol for gene knockout (KO) in m......

Development of magnetic particle-based chemiluminescence immunoassay for measurement of human procalcitonin in serum

期刊: JOURNAL OF IMMUNOLOGICAL METHODS, 2021; 488 ()

Background: Serum procalcitonin (PCT) has been recognized as a primary biomarker in bacterial infections, and monitoring its concentration could help ......

Graphene oxide-gold nanoparticle-aptamer complexed probe for detecting amyloid beta oligomer by ELISA-based immunoassay

期刊: JOURNAL OF IMMUNOLOGICAL METHODS, 2021; 489 ()

Highly sensitive and easy detection method for Alzheimer's disease (AD) with a suitable biomarker is mandatory for preventing the factors resulting fr......

Identification of tumor-associated antigens of lung cancer: SEREX combined with bioinformatics analysis

期刊: JOURNAL OF IMMUNOLOGICAL METHODS, 2021; 492 ()

The aim of this study is to identify novel tumor-associated antigens (TAAs) of lung cancer by using serological analysis of recombinant cDNA expressio......

A multi-centre study for standardization of antinuclear antibody indirect immunofluorescence screening with automated system

期刊: JOURNAL OF IMMUNOLOGICAL METHODS, 2020; 477 ()

Introduction: ndirect immunofluorescence assay (IFA) using HEp-2 as substrate plays a consolidate role for the detection and measurement of ANA, which......

Production and characterization of a neutralizing antibody against botulinum neurotoxin A

期刊: JOURNAL OF IMMUNOLOGICAL METHODS, 2020; 487 ()

As a category A toxic, the botulinum toxin(BoNT) is responsible for human botulism with an estimated lethal dose of 1 ng/kg which greatly increases th......

Application of lyophilised human platelets for antibody detection in solid phase red cell adherence assay

期刊: JOURNAL OF IMMUNOLOGICAL METHODS, 2020; 487 ()

Antibodies against human platelets cause a variety of thrombocytopenic disorders, which lead to potentially fatal haemorrhage. Therefore, their prompt......

Methodological comparison of FACS and MACS isolation of enriched microglia and astrocytes from mouse brain

期刊: JOURNAL OF IMMUNOLOGICAL METHODS, 2020; 486 ()

Microglia and astrocytes, the two innate cells in CNS, are thought to protect and remodel of synapses for proper maintenance and plasticity of neurona......

Development of a novel chemiluminescence immunoassay for the detection of procalcitonin

期刊: JOURNAL OF IMMUNOLOGICAL METHODS, 2020; 484 ()

Objective: To evaluate the analytical performance of our previously developed chemiluminescence immunoassay (CLIA) kit for the detection of procalcito......

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