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Enhanced catalytic efficiency and thermostability of glucose isomerase from Thermoanaerobacter ethanolicus via site-directed mutagenesis

期刊: ENZYME AND MICROBIAL TECHNOLOGY, 2022; 152 ()

Glucose isomerase (GI) is a key enzyme in the preparation of high fructose corn syrup (HFCS). In this study, a mutant TEGI-M-L38 M/V137 L (TEGI-M2) of......

JIF:3.409

High D-arabitol production with osmotic pressure control fed-batch fermentation by Yarrowia lipolytica and proteomic analysis under nitrogen source perturbation

期刊: ENZYME AND MICROBIAL TECHNOLOGY, 2022; 152 ()

D-arabitol, a five-carbon sugar alcohol, is widely used in food and pharmacy industry as a lower calorie sweetener or intermediate. Appropriate osmoti......

JIF:3.409

Biosynthesis of cis-3-hydroxypipecolic acid from L-lysine using an in vivo dual-enzyme cascade

期刊: ENZYME AND MICROBIAL TECHNOLOGY, 2022; 154 ()

Cis-3-Hydroxypipecolic acid (cis-3-HyPip) is an important intermediate for the synthesis of GE81112 tetrapeptides, a small family of unusual nonriboso......

JIF:3.409

Improvement of catalytic performance of endoglucanase CgEndo from Colletotrichum graminicola by site-directed mutagenesis

期刊: ENZYME AND MICROBIAL TECHNOLOGY, 2022; 154 ()

In order to improve the catalytic efficiency of cellulase for more effective utilization of lignocellulose, a novel endoglucanase (CgEndo) from Collet......

JIF:3.409

Improving the decolorization activity of Bacillus pumilus W3 CotA-laccase to Congo Red by rational modification

期刊: ENZYME AND MICROBIAL TECHNOLOGY, 2022; 155 ()

Congo Red (CR) is a typical azo dye with highly toxic and carcinogenic properties. This study aimed to improve the decolorization activity of Bacillus......

JIF:3.409

Modification to increase the thermostability and catalytic efficiency of a-L-rhamnosidase from Bacteroides thetaiotaomicron and high-level expression

期刊: ENZYME AND MICROBIAL TECHNOLOGY, 2022; 158 ()

The alpha-L-rhamnosidase BtRha from Bacteroides thetaiotao VPI-5482 is a specific enzyme that selectively hydrolyzes the alpha-1,2 glycosidic bond bet......

JIF:3.409

Enhancing the thermostability of D-allulose 3-epimerase from Clostridium cellulolyticum H10 via a dual-enzyme screening system

期刊: ENZYME AND MICROBIAL TECHNOLOGY, 2022; 159 ()

D-Allulose 3-epimerase (DAE) is promising to be used for the production of the rare sugar D-allulose in industry. However, the poor thermostability an......

JIF:3.409

Directionally modulating the product chain length of an inulosucrase by semi-rational engineering for efficient production of 1-kestose

期刊: ENZYME AND MICROBIAL TECHNOLOGY, 2022; 160 ()

Microbial inulosucrase as a transfructosylation tool has been used to produce inulin and inulin-type fructooli-gosaccharides with various polymerizati......

JIF:3.409

Catalytically active inclusion bodies (CatIBs) induced by terminally attached self-assembling coiled-coil domains: To enhance the stability of (R)-hydroxynitrile lyase

期刊: ENZYME AND MICROBIAL TECHNOLOGY, 2022; 153 ()

The catalytically-active inclusion bodies (CatIBs) represent a promising strategy for immobilizing enzyme without additional carriers and chemicals, w......

JIF:3.409

Development of a novel expression platform for heterologous protein production via deleting the p53-like regulator Vib1 in Trichoderma reesei

期刊: ENZYME AND MICROBIAL TECHNOLOGY, 2022; 155 ()

Trichoderma reesei is widely used as a protein production host due to its high natural capacity to secrete enzymes. Nonetheless, the complexity and ab......

JIF:3.409

A simple and green method for direct determination of hydrogen peroxide and hypochlorite in household disinfectants based on personal glucose meter

期刊: ENZYME AND MICROBIAL TECHNOLOGY, 2022; 155 ()

A convenient and green method was developed using a personal glucose meter (PGM) for direct determination of hydrogen peroxide and hypochlorite in hou......

JIF:3.409

Gene cloning of a highly active phytase from Lactobacillus plantarum and further improving its catalytic activity and thermostability through protein engineering

期刊: ENZYME AND MICROBIAL TECHNOLOGY, 2022; 156 ()

Phytase belongs to orthophosphate monoester hydrolase, which can catalyze the gradual hydrolysis of phytic acid to inositol phosphate. It can be added......

JIF:3.409

Improving (2S)-naringenin production by exploring native precursor pathways and screening higher-active chalcone synthases from plants rich in flavonoids

期刊: ENZYME AND MICROBIAL TECHNOLOGY, 2022; 156 ()

Flavonoids are a group of valuable compounds with a variety of health benefits. (2 S)-Naringenin is an important flavonoid skeleton, which can be tail......

JIF:3.409

Adenylation domains of nonribosomal peptide synthetase: A potential biocatalyst for synthesis of dipeptides and their derivatives

期刊: ENZYME AND MICROBIAL TECHNOLOGY, 2022; 160 ()

Dipeptides and their derivatives are important functional compounds that can be applied to fields such as medicine and food. As biological macromolecu......

JIF:3.409

Ascorbate oxidase enabling glucometer readout for portable detection of hydrogen peroxide

期刊: ENZYME AND MICROBIAL TECHNOLOGY, 2022; 160 ()

A rapid, portable, and cost-effective method using personal glucose meter (PGM) for quantitative analysis of hydrogen peroxide (H2O2) was established ......

JIF:3.409

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