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Structural Flexibility of Peripheral Loops and Extended C-terminal Domain of Short Length Substrate Binding Protein from Rhodothermus marinus

期刊: PROTEIN JOURNAL, 2021; 40 (2)

Substrate binding proteins (SBPs) bind to specific ligands in the periplasmic regions of cells and then bind to membrane proteins to participate in tr......

QSSR Modeling of Bacillus Subtilis Lipase A Peptide Collision Cross-Sections in Ion Mobility Spectrometry: Local Descriptor Versus Global Descriptor

期刊: PROTEIN JOURNAL, 2021; 40 (1)

To investigate the structure-dependent peptide mobility behavior in ion mobility spectrometry (IMS), quantitative structure-spectrum relationship (QSS......

Separation of Storage Proteins (7S and 11S) from Soybean Seed, Meals and Protein Isolate Using an Optimized Method Via Comparison of Yield and Purity

期刊: PROTEIN JOURNAL, 2021; 40 (3)

The primary purpose of this study was to extract beta-conglycinin (7S) and glycinin (11S) from soybean seed, soybean meals and soybean protein isolate......

UMAP-DBP: An Improved DNA-Binding Proteins Prediction Method Based on Uniform Manifold Approximation and Projection

期刊: PROTEIN JOURNAL, 2021; 40 (4)

DNA-binding proteins play a vital role in cellular processes. It is an extremely urgent to develop a high-throughput method for efficiently identifyin......

Exploration of Serum Marker Proteins in Mice Induced by Babesia microti Infection Using a Quantitative Proteomic Approach

期刊: PROTEIN JOURNAL, 2021; 40 (1)

Babesia microti is a protozoan that mainly parasitizes rodent and human erythrocytes. B. microti infection can result in changes in the expression lev......

Protein Analysis of Pollen Tubes after the Treatments of Membrane Trafficking Inhibitors Gains Insights on Molecular Mechanism Underlying Pollen Tube Polar Growth

期刊: PROTEIN JOURNAL, 2021; 40 (2)

Pollen tube elongation is characterized by a highly-polarized tip growth process dependent on an efficient vesicular transport system and largely mobi......

Expression and Purification of a Recombinant Enterotoxin Protein Using Different E. coli Host Strains and Expression Vectors

期刊: PROTEIN JOURNAL, 2021; 40 (2)

Infection by Enterotoxigenic Escherichia coli is a common cause of diarrhea in animals. The development of vaccines against enterotoxins can effective......

Maltose Induced Expression of Cecropin AD by SUMO Technology inBacillus subtilisWB800N

期刊: PROTEIN JOURNAL, 2020; 39 (4)

Cecropin AD (CAD) is a hybrid peptide composed of 37 amino acids with the characters of strong antibacterial, antitumor properties and no hemolytic ac......

Cereblon Promotes the Ubiquitination and Proteasomal Degradation of Interleukin Enhancer-Binding Factor 2

期刊: PROTEIN JOURNAL, 2020; 39 (5)

Interleukin enhancer-binding factor 2 (ILF2) forms a heterodimer with interleukin enhancer-binding factor 3 (ILF3) via double-stranded RNA-binding mot......

The Last Secret of Protein Folding: The Real Relationship Between Long-Range Interactions and Local Structures

期刊: PROTEIN JOURNAL, 2020; 39 (5)

The protein folding problem has been extensively studied for decades, and hundreds of thousands of protein structures have been solved. Yet, how prote......

Studying the Role of a Single Mutation of a Family 11 Glycoside Hydrolase Using High-Resolution X-ray Crystallography

期刊: PROTEIN JOURNAL, 2020; 39 (6)

XynII is a family 11 glycoside hydrolase that uses the retaining mechanism for catalysis. In the active site, E177 works as the acid/base and E86 work......

Integrated In Silico-In Vitro Identification and Optimization of Bone Morphogenic Protein-2 Armpit Epitope as Its Antagonist Binding Site

期刊: PROTEIN JOURNAL, 2020; 39 (6)

Bone morphogenic protein-2 (BMP-2) is the most documented member of BMP family and plays a crucial role in bone formation and growth. In this study, w......

Anticancer Actions of Azurin and Its Derived Peptide p28

期刊: PROTEIN JOURNAL, 2020; 39 (2)

Cancers are a great threat to humans. In cancer therapy, surgical removal of the tumor combined with radiotherapy and chemotherapy is the most routine......

Soluble Expression of Recombinant Human Cystatin C and Comparison of the Ni Column and Magnetic Bead Purification

期刊: PROTEIN JOURNAL, 0; ()

Cystatin C, also known as gamma-trace or post-gamma-globulin, is a cysteine protease inhibitor from the cystatin superfamily. It is usually used as a ......

Grafting, Stripping and Stapling of Helical Peptides from the Dimerization Interface of ONFH-Related Bone Morphogenetic Protein-2

期刊: PROTEIN JOURNAL, 2019; 38 (1)

Transforming growth factor-/bone morphogenetic protein (TGF-/BMP) signaling plays a fundamental role in embryonic skeletal development and postnatal b......

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