Morphology and TUNEL labeling of lungs of Dox-treated transgenic mice at E19 and P7. results

Am J Pathol. 2008 July; 173(1): 42–56.

Figure 4 Morphology and TUNEL labeling of lungs of Dox-treated transgenic mice at E19 and P7. A: CCSP+/FasL+ lungs at E19 (late gestation, saccular stage of development) showing abundant cellular debris within the airspaces and pyknotic nuclei within the bronchial epithelium. Br, bronchus. B: Lungs of CCSP+/FasL− littermate of A without histopathological evidence of apoptosis. C: TUNEL labeling of Dox-treated CCSP+/Fas+ lungs at E19 showing abundant aggregates of TUNEL-positive nuclear material within the alveolar septa and airspaces. In addition, TUNEL-positive nuclei are noted within the bronchial epithelium. Br, bronchus. D: Lungs of a CCSP+/FasL− littermate containing only rare TUNEL-positive nuclei, localized to peribronchial and perivascular stromal cells. E: CCSP+/FasL+ lungs at P7 showing relatively large airspaces, little evidence of secondary crest formation, and abundant intra-alveolar macrophages admixed with apoptotic nuclear debris. Septa appear relatively hypercellular. F: Lungs of CCSP+/FasL− littermate of C showing thinner septa, more advanced secondary crest formation (early alveolar stage), and only scant intra-alveolar macrophages. G: CCSP+/FasL+ lungs at P7 showing persistently high TUNEL positivity, mainly within intra-alveolar cellular debris. H: Lungs of CCSP+/FasL− littermate at P7 showing very low TUNEL reactivity. I: Apoptotic index. Values represent mean ± SD of at least four animals per group. *P < 0.001 versus Dox-treated CCSP+/FasL− littermates. H&E staining (A, B, E, F); TUNEL-FITC labeling (C, D, G, H). Original magnifications, ×400. Am J Pathol. 2008 July; 173(1): 42–56.

TLR3 and TLR4 protein is localised to endometrial cells during the menstrual cycle results

Reproductive Biology and Endocrinology 2008, 6:40

TLR3 and TLR4 protein is localised to endometrial cells during the menstrual cycle. TLR3 protein staining in healthy late proliferative (LP) tissue was high in luminal and glandular tissue (A, brown precipitate) and lower in LP endometriotic tissue (B). Late secretory (LS) endometrium showed highly expressed TLR3 in the epithelium (C), but weakly in endometriosis (D). Intense staining of TLR4 proteins was shown in mid proliferative (MP) tissue (E). In late proliferative phase of endometriosis, TLR4 proteins were comparably lower (F). TLR4 protein was high in mid secretory (MS) normal endometrium (G), whereas it was decreased in endometriotic MS tissue (H). During the menstrual phase, both TLR3 (I) and TLR4 (J) were highly expressed. Co-immunostaining for TLR4 (green), CD14 (K, red) and CD163 (L, red) demonstrated that TLR4 proteins were expressed by CD14 positive dendritic cells and monocytes (K, yellow) and by CD163 positive macrophages (L, yellow). Localisation of TLR4 to immune cells is marked by a black arrow (J) and by white arrows (K, L). Allhorn et al. Reproductive Biology and Endocrinology 2008 6:40