Am J Pathol. 2008 July; 173(1): 30–41.
Antibodies and Reagents The primary antibodies were obtained from different sources: anti-RANTES (sc-1410), anti-tumor necrosis factor (TNF)-α (sc-8301), anti-IκBβ (sc-946), anti-interleukin (IL)-6 (sc-7920), and anti-actin (sc-1616) (Santa Cruz Biotechnology, Santa Cruz, CA); anti-phospho-Akt (Ser473), anti-Akt, anti-phospho-IκBα (Ser32/36), anti-IκBα, anti-phospho-p65 NF-κB (Ser536), anti-p65 NF-κB, anti-phospho-GSK-3β (Ser9), anti-GSK-3β (Cell Signaling Technology, Beverly, MA), and anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (Ambion, Austin, TX). Recombinant human HGF, TNF-α, and IL-1β were purchased from R&D Systems (Minneapolis, MN). Cell culture media, newborn calf serum, and supplements were obtained from Invitrogen (Carlsbad, CA). Chemical inhibitor wortmannin and cell-permeable inhibitor peptide NF-κB SN50 were purchased from Calbiochem (La Jolla, CA). All other chemicals were of analytic grade and were obtained from Sigma (St. Louis, MO) or Fisher (Pittsburgh, PA) unless otherwise indicated.
