Bacterial Growth Assay methods

Plant Physiol. 2009 February; 149(2): 1017–1027

Bacterial Growth Assay Plant inoculations and bacterial growth assays were performed as previously described (Tornero and Dangl, 2001; Yun et al., 2006). Briefly, 3-week-old plants were infected with bacterial suspensions (108 cfu/mL except where indicated) resuspended in 10 mm MgCl2, and Silwet L-77 (200 μL/L). The aerial part of plants growing in pots were submerged upside down in the bacterial solution for 30 s and then covered with a transparent lid. Two hours after inoculation, the lid was removed and the collected sample was considered as zero time point; samples were taken every 24 h over 4 d.