Am J Pathol. 2008 July; 173(1): 42–56.
Figure 8 Gross and microscopic appearance of lungs of Dox-treated transgenic mice at P21. A and B: Macroscopic appearance of formalin-inflated lungs of Dox-treated CCSP+/FasL+ and CCSP+/FasL− mice at P21. Lungs of double-transgenic pups appeared large and pale with obtunded edges. C and D: Representative photomicrographs of lungs of Dox-treated CCSP+/FasL+ and CCSP+/FasL− mice at P21. C: CCSP+/FasL+ lungs at P21 showing large-sized simple alveolar spaces separated by thin alveolar septa. Small macrophage collections are present. D: Lungs of CCSP+/FasL− littermate of C showing a complex network of small-sized alveoli (advanced alveolar stage). H&E staining. Original magnifications, ×400. Am J Pathol. 2008 July; 173(1): 42–56.
J. Clin. Invest. 118(1): 124-132 (2007).
Histology. Hearts were fixed in 4% paraformaldehyde in phosphate-buffered saline, embedded in paraffin, and sectioned at 5-μm intervals. H&E staining, Masson’s trichrome staining, and picrosirius red staining were performed using standard procedures.
J. Clin. Invest. 118(1): 124-132 (2007).
Measurements of mean cardiomyocyte area and myocardial fibrosis. Cardiomyocyte size was assessed on H&E-stained sections using ImageJ software (http://rsb.info.nih.gov/ij/) (NIH). About 100–150 randomly chosen cardiomyocytes from each group (n = 2–3) were analyzed to measure cross-sectional cardiomyocyte area. ImageJ software (NIH) was used to measure the amount of myocardial fibrosis on picrosirius red stained sections. About 30–50 randomly chosen frames from each group (n = 2–3) were analyzed.
