Recombinant adenovirus infection and immunoblotting analysis. The replication-deficient recombinant adenoviral vectors encoding Cre/LoxP inducible HA-Bax (Ad/Bax), Cre recombinase (Ad/Cre), or luciferase (Ad/Luc) were generated as described previously (69, 70). All recombinant adenoviruses were propagated in HEK293 cells, purified, and titrated by standard protocols (59). For viral infection, the ratio of Ad/Bax to Ad/Cre was 5:1 at a total multiplicity of infection of 50 −100 (70). In control experiments, Ad/Luc (luciferase) plus Ad/Cre or Ad/Bax plus Ad/GFP (green fluorescent protein) were kept at the same ratio.