笔记详情
标题
Reverse Transcription Experiments.
内容
Reverse Transcription Experiments. Total RNA was extracted from Arabidopsis leaves by using TRIzol reagent (Invitrogen). After conversion to first-strand cDNA, KAT2 and EF1α were amplified by PCR from the same amounts of cDNA by using the following couples of primers: KAT2-3000 (5′-gcgtcttagacgagttagctcgc-3′) and KAT2-3930 (5′-ccgtgaaataggtagacgttctgaacgattggg-3′) or EF1α-350 (5′-ccaccactggtggttttgaggctggtatc-3′) and EF1α-900 (5′-cattgaacccaacgttgtcacctggaag-3′).
点击翻译来源
Proc Natl Acad Sci U S A. 2008 April 1; 105(13): 5271–5276
类别
领域
Plant Science
