Reverse Transcription-PCR桾otal RNAs were isolated from MCF7 cells, which were uninduced or induced to express Brg1 siRNA for 3 days along with or without treatment of 0.5 ?FONT size=-2>M doxorubicin for 8 h. First-strand cDNA was synthesized by using iScript according to the instructions of the manufacturer (Bio-Rad). A 160-bp cDNA fragment of p21 was amplified with forward primer 5'-CATGTGGACCTGTCACTGTC-3' and the reverse primer 5'-CCTCTTGGAGAAGATCAGCC-3'.A 225-bp cDNA fragment of actin was measured as a loading control, which was amplified with forward primer 5'-CTGAAGTACCCCATCGAGCACGGCA-3' and the reverse primer 5'-GGATAGCACAGCCTGGATAGCAACG-3'.