笔记详情
标题
Cell Proliferation
内容
Cell Proliferation—INS832/13 cell proliferation was evaluated using a BrdUrd3 enzyme-linked immunoassay kit (Roche Applied Science). Adenovirus-transduced INS832/13 cells were seeded in 96-well plates (8 x 104 cells/well) and allowed to recover for 24 h. BrdUrd was added to the culture medium for 1 h, and cells were fixed, incubated with peroxidase-conjugated anti-BrdUrd antibody, and the immune complexes were quantified by enzyme-linked immunosorbent assay (Bio-Rad).
点击翻译来源
J. Biol. Chem., Vol. 282, Issue 1, 287-293
类别
领域
Cell Biology
关键词
