笔记详情
标题
Isolation of the T-DNA-Tagged Mutant kat2-1 Disrupted in the KAT2 Gene.
内容
Isolation of the T-DNA-Tagged Mutant kat2-1 Disrupted in the KAT2 Gene. The kat2-1 knockout line was obtained by PCR screening of 40,000 Arabidopsis thaliana T-DNA insertion mutants [Wassilevskija ecotype; library constructed by Institut National de la Recherche Agronomique, Versailles (27)]. A Southern blot with a probe targeting the T-DNA right border revealed a single insertion locus. The exact position of the T-DNA insertion was determined by sequencing the T-DNA flanking sequences. Plants homozygous for the disruption were selected by PCR in the F3 progeny.
点击翻译来源
Proc Natl Acad Sci U S A. 2008 April 1; 105(13): 5271–5276
类别
领域
Plant Science
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