笔记详情
标题
cell culture and stable cell lines.
内容
Cell culture and stable cell lines. Human prostate cancer cell LNCaP sublines 104-S, 104-R1, and CDXR were generated and maintained as described previously (36). Androgen-dependent 104-S cells were grown in Dulbecco modified Eagle medium with 10% fetal bovine serum, supplemented with 1 nM DHT (5α-dihydrotestosterone). Androgen-independent 104-R1, CDXR, and PC3 cells were cultured in Dulbecco modified Eagle medium with 10% dextran-coated, charcoal-stripped fetal bovine serum (27).
点击翻译来源
mol cell biol. 2006 march 26(5): 1908–1916
类别
领域
cell biology
