笔记详情
标题
Cell counting and TB exclusion assays
内容
Cell counting and TB exclusion assays. 7.0 × 104 cells were seeded in 60-mm plates 1 day prior to initiation of experiment. Starting at day 0, cells were trypsinized and stained with 0.4% TB solution (Sigma-Aldrich), and both viable and dead cells were counted in a Bürker chamber. Every other day, starting at day 0, cultures for subsequent counting were supplemented with fresh media containing 1% FCS, PEST, and DMSO or DAPT. Each experiment was performed in triplicate and repeated twice.
点击翻译来源
J. Clin. Invest. 118(1): 217-228 (2007).
类别
领域
Cancer/Oncology
