Expression of Ezh2 in hematopoietic cells. (A) To isolate the different lineage-negative (Lin^-) populations from BM, the 5% most Lin^- cells were selected. (B) Cells in the Lin^- population were sorted based on Sca-1 and c-kit surface markers. (C) Expression of Ezh2 as measured by qPCR in the different Sca-1 and c-kit populations relative to Sca-1^-c-kit^- BM cells. (D) Growth of Lin^-Sca-1⁺c-kit⁺ cells cultured in the presence of GM-CSF and SCF. (E) Relative expression of Ezh2 was monitored by qPCR at different time points after initiation of differentiation with GM-CSF and SCF. Day 0 was set at 1. Insert shows gel analysis of amplified RT-PCR products.