Defeating Major Contaminants in Fe3+- Immobilized Metal Ion Affinity Chromatography (IMAC) Phosphopeptide Enrichment

Potel, CM; Lin, MH; Heck, AJR; Lemeer, S

Lemeer, S (reprint author), Univ Utrecht, Biomol Mass Spectrometry & Prote, Padualaan 8, NL-3584 CA Utrecht, Netherlands.

MOLECULAR & CELLULAR PROTEOMICS, 2018; 17 (5): 1028

Abstract

Here we demonstrate that biomolecular contaminants, such as nucleic acid molecules, can seriously interfere with immobilized metal ion affinity chroma......

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