Single-molecule detection of deoxyribonucleoside triphosphates in microdroplets

Breiner, B; Johnson, K; Stolarek, M; Silva, AL; Negrea, A; Bell, NM; Isaac, TH; Dethlefsen, M; Chana, J; Ibbotson, LA; Palmer, RN; Bush, J; Dunning, AJ; Love, DM; Pachoumi, O; Kelly, DJ; Shibahara, A; Wu, M; Sosna, M; Dear, PH; Tolle, F; Petrini, E;

Frayling, CA (reprint author), Base4 Innovat Ltd, Broers Bldg,21 JJ Thomson Ave, Cambridge CB3 0FA, England.

NUCLEIC ACIDS RESEARCH, 2019; 47 (17):

Abstract

A new approach to single-molecule DNA sequencing in which dNTPs, released by pyrophosphorolysis from the strand to be sequenced, are captured in micro......

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