FLEP-seq: simultaneous detection of RNA polymerase II position, splicing status, polyadenylation site and poly(A) tail length at genome-wide scale by single-molecule nascent RNA sequencing-MedSci.cn

FLEP-seq: simultaneous detection of RNA polymerase II position, splicing status, polyadenylation site and poly(A) tail length at genome-wide scale by single-molecule nascent RNA sequencing

Long, YP; Jia, JB; Mo, WP; Jin, XH; Zhai, JX

Zhai, JX (corresponding author), Southern Univ Sci & Technol, Sch Life Sci, Inst Plant & Food Sci, Key Lab Mol Design Plant Cell Factory Guangdong H, Shenzhen, Peoples R China.

NATURE PROTOCOLS, 2021; 16 (9): 4355

Abstract

Elongation, splicing and polyadenylation are fundamental steps of transcription, and studying their coordination requires simultaneous monitoring of t......

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