Polyvinylidene fluoride (PVDF) has attracted much attention due to its biocompatibility, piezoelectric and favourable mechanical properties. The modification of polypyrrole coating on the surface of the PVDF film by electrochemical method was studied. The surface morphology of the nanostructured polypyrrole coating modified PVDF film was observed by scanning electron microscopy (SEM) and atomic force microscope (AFM). The surface contact angle analyzer and AFM were used to study the hydrophilicity and electrical properties of the PVDF membrane modified by the nano structure polypyrrole coating. The biocompatibility of the nanostructured polypyrrole coating modified PVDF membrane was investigated by in vitro mineralization, cell viability staining and CCK-8 assay, respectively. The results show that the surface hydrophilicity, surface potential distribution and biocompatibility of PVDF films are significantly improved by the modification of the nanostructured polypyrrole coating, and more conducive to the surface bone mineral deposition and cell adhesion and proliferation. In addition, the PVDF membrane modified by polypyrrole nanowire has higher hydrophilicity and surface potential distribution, which is more conducive to cell adhesion and proliferation.