Target-dependent nickase activities of the CRISPR-Cas nucleases Cpf1 and Cas9

Fu, BXH; Smith, JD; Fuchs, RT; Mabuchis, M; Curcuru, J; Robb, GB; Fire, AZ

Fu, BXH; Fire, AZ (reprint author), Stanford Univ, Dept Pathol, Sch Med, Stanford, CA 94305 USA.; Fu, BXH; Fire, AZ (reprint author), Stanford Univ, Dept Genet, Sch Med, Stanford, CA 94305 USA.; Robb, GB (reprint author), New England BioLabs Inc, Ipswich,

NATURE MICROBIOLOGY, 2019; 4 (5): 888

Abstract

Clustered regularly interspaced short palindromic repeats (CRISPR) machineries are prokaryotic immune systems that have been adapted as versatile gene......

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