CRISPR/Cas12a-Based Versatile Method for Checking Quantitative Polymerase Chain Reaction Samples with Cycles of Threshold Values in the Gray Zone

Chen, YJ; Wu, SY; Wu, H; Cheng, P; Wang, XF; Qian, SWJ; Zhang, MY; Xu, JF; Ji, F; Wu, J

Wu, J (corresponding author), Zhejiang Univ, Coll Biosyst Engn & Food Sci, Hangzhou 310058, Peoples R China.; Xu, JF (corresponding author), Zhejiang Acad Agr Sci, State Key Lab Managing Biot & Chem Threats Qual &, Hangzhou 310021, Peoples R China.; Ji, F (corresponding author), Zhejiang Univ, Affiliated Hosp 1, Sch Med, Hangzhou 310003, Zhejiang, Peoples R China.; Wu, J (corresponding author), Minist Agr, Key Lab Site Proc Equipment Agr Prod, Hangzhou 310058, Peoples R China.

ACS SENSORS, 2021; 6 (5): 1963

Abstract

Quantitative polymerase chain reaction (qPCR) is widely applied in foodborne pathogen detection and diagnosis. According to the cycles of threshold (C......

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