High-throughput creation of Nicotiana tabacum gene-targeted mutants based on CRISPR/Cas9

Chen, YD; Xiang, HY; Jia, L; Yang, QX; Zhang, JD; Jiang, JR; Zeng, WL; Deng, LL; Jin, JJ; Gao, Q; Li, XM

Gao, Q; Li, XM (通讯作者)，Yunnan Acad Tobacco Sci, Kunming 650106, Peoples R China.

PLANT CELL REPORTS, 2023; 42 (12): 2039

Abstract

Key message4382 available sgRNAs targeting 1060 tobacco genes were obtained, and 10,682 targeted mutants were created using high-throughput methods. F......

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