Formation and nucleolytic processing of Cas9-induced DNA breaks in human cells quantified by droplet digital PCR

Dibitetto, D; La Monica, M; Ferrari, M; Marini, F; Pellicioli, A

Pellicioli, A (reprint author), Univ Milan, Dept Biosci, I-20131 Milan, Italy.

DNA REPAIR, 2018; 68 (): 68

Abstract

Cas9 endonuclease from S. pyogenes is widely used to induce controlled double strand breaks (DSB) at desired genomic loci for gene editing. Here, we d......

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