Multicopy Chromosomal Integration Using CRISPR-Associated Transposases

Zhang, YW; Sun, XM; Wang, QZ; Xu, JQ; Dong, F; Yang, SQ; Yang, JW; Zhang, ZX; Qian, Y; Chen, J; Zhang, J; Liu, YM; Tao, RS; Jiang, Y; Yang, JJ; Yang, S

Yang, JJ; Yang, S (corresponding author), Chinese Acad Sci, CAS Ctr Excellence Mol Plant Sci, Key Lab Synthet Biol, Shanghai 200032, Peoples R China.; Yang, S (corresponding author), Shanghai Inst Biol Sci, Huzhou Ctr Ind Biotechnol, Huzhou 313000, Peoples R China.

ACS SYNTHETIC BIOLOGY, 2020; 9 (8): 1998

Abstract

Controlling the copy number of gene expression cassettes is an important strategy to engineer bacterial cells into high-efficiency biocatalysts. Curre......

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