Proteomics study of serum exosomes in Kawasaki disease patients with coronary artery aneurysms

Xie, XF; Chu, HJ; Xu, YF; Hua, L; Wang, ZP; Huang, P; Jia, HL; Zhang, L

Zhang, L (reprint author), Guangzhou Med Univ, Guangzhou Women & Childrens Med Ctr, Dept Pediat Cardiol, 318 Renmin Rd, Guangzhou 510120, Guangdong, Peoples R China.; Jia, HL (reprint author), Jinan Univ, Coll Life Sci & Technol, Inst Life & Hlth Engn, Ke

CARDIOLOGY JOURNAL, 2019; 26 (5): 584

Abstract

Background: To study the protein profile of the serum exosomes of patients with coronary artery aneurysms (CAA) caused by Kawasaki disease (KD). Methods: Two-dimensional electrophoresis (2-DE) was used to identify proteins from the exosomes of serum obtained from children with CAA caused by KD, as well as healthy controls. Differentially expressed proteins were identified using matrix-assisted laser desorption/ionization time-of-flight/time-of-flight mass spectrometry (MALDI-TOF/TOF MS) analysis. Results: Thirty two differentially expressed proteins were identified (18 up-regulated and 14 downregulated) from serum exosomes of children with CAA and were compared to healthy controls. The expression levels of 4 proteins (TN RBP4, LRG1, and APOA4) were validated using Western blotting. Classification analysis and protein-protein network analysis showed that they are associated with multiple functional groups, including host immune response, inflammation, apoptotic process, developmental process, and biological adhesion process. Conclusions: These findings establish a comprehensive proteomic profile of serum exosomes from children with CAA caused by KD, and provide additional insights into the mechanisms of CAA caused by KD.

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