Reverse transcriptase kinetics for one-step RT-PCR

Rejali, NA; Zuiter, AM; Quackenbush, JF; Wittwer, CT

Wittwer, CT (corresponding author), Univ Utah, Med Sch, Dept Pathol, Room 266,383 Colorow Dr, Salt Lake City, UT 84108 USA.

ANALYTICAL BIOCHEMISTRY, 2020; 601 ():

Abstract

Understanding reverse transcriptase (RT) activity is critical for designing fast one-step RT-PCRs. We report a stopped-flow assay that monitors SYBR G......

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