High-throughput mapping of CoA metabolites by SAMDI-MS to optimize the cell-free biosynthesis of HMG-CoA

O'Kane, PT; Dudley, QM; McMillan, AK; Jewett, MC; Mrksich, M

Jewett, MC; Mrksich, M (reprint author), Northwestern Univ, Ctr Synthet Biol, Evanston, IL 60208 USA.; Mrksich, M (reprint author), Northwestern Univ, Dept Chem, 2145 Sheridan Rd, Evanston, IL 60208 USA.; Jewett, MC (reprint author), Northwestern Univ, De

SCIENCE ADVANCES, 2019; 5 (6):

Abstract

Metabolic engineering uses enzymes to produce small molecules with industrial, pharmaceutical, and energy applications. However, efforts to optimize e......

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