Temporal Analysis of Conformers in the Course of pH Scan Directed by Urea-Urease Reaction-A "Protein Clock"

Chang, CM; Prabhu, GRD; Tseng, CM; Urban, PL

Urban, PL (reprint author), Natl Tsing Hua Univ, Dept Chem, 101,Sect 2,Kuang Fu Rd, Hsinchu 30013, Taiwan.; Urban, PL (reprint author), Natl Tsing Hua Univ, Frontier Res Ctr Fundamental & Appl Sci Matters, 101,Sect 2,Kuang Fu Rd, Hsinchu 30013, Taiwan.

ANALYTICAL CHEMISTRY, 2019; 91 (14): 8814

Abstract

We demonstrate an analytical approach to monitor the effect of pH on protein tertiary structure. An autocatalytic enzyme reaction is used to stimulate......

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