Promiscuity of host cell proteins in the purification of histidine tagged recombinant xylanase A by IMAC procedures: A case study with a Ni2+-tacn-based IMAC system

Yang, YZ; Mitri, K; Zhang, CF; Boysen, RI; Hearn, MTW

Hearn, MTW (reprint author), Monash Univ, Sch Chem, Australian Ctr Res Separat Sci ACROSS, Monash, Vic 3800, Australia.; Hearn, MTW (reprint author), Monash Univ, Sch Chem, POB 3800, Melbourne, Vic 3800, Australia.

PROTEIN EXPRESSION AND PURIFICATION, 2019; 162 (): 51

Abstract

Determination of the extent of host cell protein (HCP) contamination is an essential pre-requisite to validate the chromatographic purification of rec......

Full Text Link