Unbiased quantification of immunoglobulin diversity at the DNA level with VDJ-seq

Chovanec, P; Bolland, DJ; Matheson, LS; Wood, AL; Krueger, F; Andrews, S; Corcoran, AE

Corcoran, AE (reprint author), Babraham Inst, Nucl Dynam Programme, Babraham Res Campus, Cambridge, England.; Corcoran, AE (reprint author), Babraham Inst, Lab Lymphocyte Signalling & Dev, Babraham Res Campus, Cambridge, England.

NATURE PROTOCOLS, 2018; 13 (6): 1232

Abstract

For high-throughput sequencing and quantification of immunoglobulin repertoires, most methodologies use RNA. However, output varies enormously between......

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