Quantification of 3-ketodihydrosphingosine using HPLC-ESI-MS/MS to study SPT activity in yeast Saccharomyces cerevisiae

Ren, JH; Snider, J; Airola, MV; Zhong, A; Rana, NA; Obeid, LM; Hannun, YA

Hannun, YA (reprint author), SUNY Stony Brook, Dept Med, Stony Brook, NY 11794 USA.; Hannun, YA (reprint author), SUNY Stony Brook, Dept Biochem & Cell Biol, Stony Brook, NY 11794 USA.; Hannun, YA (reprint author), SUNY Stony Brook, Stony Brook Canc Ctr,

JOURNAL OF LIPID RESEARCH, 2018; 59 (1): 162

Abstract

Serine palmitoyltransferase (SPT) catalyzes the rate-limiting step of condensation of L-serine and palmitoyl-CoA to form 3-ketodihydrosphingosine (3KD......

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