更新:研究者成功重复韩春雨 NgAgo 方法

2016-07-16 岳东晓 岳东晓

韩氏 NgAgo 基因编辑法已经 通过PCR 验证在室温下成功用于编辑哺乳动物(老鼠)基因,研究者确认多处基因编辑-删除,并认为效率甚高(应该是与 CRISPR-Cas9 相比)。我不是内行,行话也是临时学,但字还是认识的,大家自己看啊。

韩氏 NgAgo 基因编辑法已经 通过PCR 验证在室温下成功用于编辑哺乳动物(老鼠)基因,研究者确认多处基因编辑-删除,并认为效率甚高(应该是与 CRISPR-Cas9 相比)。我不是内行,行话也是临时学,但字还是认识的,大家自己看啊。参见前文:《最新:研究者成功重复韩氏 NgAgo 方法》后我对事件的评论。下面是部分洋文的翻译。

【OK。讲座之后我在这发表了用 (韩氏)NgAgo 编辑老鼠的第一个 PCR 结果 --> 效率高!!!】(三个惊叹号系原文),

【你应该试试,非常高效( PCR没有用 T7)】(You should try, this is the way to go. Highly efficient (the PCR was done without T7) 。

【正如那篇(韩春雨)的重大论文(seminal paper),(NgAgo)产生了多处基因编辑->删除】

 Not really. I used one oligo. As per the seminal paper creates multiple editing => deletion



仔细看看:确认能在室温工作。


 OK following the talk I put here my first PCR results on  editing in mice using NgAg -> Efficient !!!


履历似乎比一篇 JBC (有质疑)的 方博高出1.5个量级

Dr Gaétan Burgio

GROUP LEADER
Room 2.228

+61 2 612 59428




版权声明:
本网站所有内容来源注明为“梅斯医学”或“MedSci原创”的文字、图片和音视频资料,版权均属于梅斯医学所有。非经授权,任何媒体、网站或个人不得转载,授权转载时须注明来源为“梅斯医学”。其它来源的文章系转载文章,或“梅斯号”自媒体发布的文章,仅系出于传递更多信息之目的,本站仅负责审核内容合规,其内容不代表本站立场,本站不负责内容的准确性和版权。如果存在侵权、或不希望被转载的媒体或个人可与我们联系,我们将立即进行删除处理。
在此留言
评论区 (11)
#插入话题
  1. [GetPortalCommentsPageByObjectIdResponse(id=2032738, encodeId=b97f2032e3873, content=<a href='/topic/show?id=ff85e343730' target=_blank style='color:#2F92EE;'>#研究者#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=25, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=73437, encryptionId=ff85e343730, topicName=研究者)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=1a19115, createdName=stfoxst, createdTime=Thu Oct 13 21:55:00 CST 2016, time=2016-10-13, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=100530, encodeId=438b100530cb, content=嗨,老方老脑筋,实验验证了?振动贴, beContent=null, objectType=article, channel=null, level=null, likeNumber=70, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=http://cacheapi.medsci.cn/resource/upload/20160601/IMG574E13FEE55F33222.jpg, createdBy=929c1478678, createdName=zibozhouping, createdTime=Thu Aug 18 20:58:00 CST 2016, time=2016-08-18, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=93361, encodeId=9b9e9336113, content=方舟子这个傻逼该被打脸了, beContent=null, objectType=article, channel=null, level=null, likeNumber=97, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=9d211716813, createdName=王抠抠, createdTime=Mon Jul 18 18:54:00 CST 2016, time=2016-07-18, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=93350, encodeId=275993350ba, content=韩教授威武!, beContent=null, objectType=article, channel=null, level=null, likeNumber=93, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=93f31631454, createdName=1dd8c52fm63(暂无匿称), createdTime=Mon Jul 18 12:22:00 CST 2016, time=2016-07-18, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1464056, encodeId=3132146405681, content=<a href='/topic/show?id=301812e562e' target=_blank style='color:#2F92EE;'>#NgAgo#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=0, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=12756, encryptionId=301812e562e, topicName=NgAgo)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=72c16575610, createdName=zhlpower, createdTime=Mon Jul 18 07:55:00 CST 2016, time=2016-07-18, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1550911, encodeId=dc8a15509116c, content=<a href='/topic/show?id=662810003e92' target=_blank style='color:#2F92EE;'>#韩春雨#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=33, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=100037, encryptionId=662810003e92, topicName=韩春雨)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=db0a14143395, createdName=bshuang, createdTime=Mon Jul 18 07:55:00 CST 2016, time=2016-07-18, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=93314, encodeId=f7a3933142f, content=这能对方舟子呵呵了,打脸, beContent=null, objectType=article, channel=null, level=null, likeNumber=90, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=http://thirdwx.qlogo.cn/mmopen/vi_32/Q0j4TwGTfTIKlpu9buib1TxXRibfFuzYXHaLxQgfUpwzYwTMo8551BS40KVZNRDLWmAXSibjfnFm0QggOrCSWElfw/132, createdBy=95aa1617853, createdName=happy1, createdTime=Mon Jul 18 07:03:00 CST 2016, time=2016-07-18, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=93241, encodeId=e9cd9324169, content=不要将一个严肃的学术问题沦落到方舟子的屁股问题. <br> 对于学术的质疑, 尤其是做过实验人得质疑, 必须严肃答复的. 当面对质疑潮时, 一个严肃的学者是必须强硬答复的. <br> Peer review的前提是文责自负, 否则哪里会有撤稿现象., beContent=null, objectType=article, channel=null, level=null, likeNumber=93, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=c4a5105539, createdName=lovetcm, createdTime=Sun Jul 17 13:26:00 CST 2016, time=2016-07-17, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=93240, encodeId=da2393240b7, content=一位来自印度基因与综合生物学研究所的Debojyoti Chakraborty博士向媒体确认“this system works”(这一系统奏效了)。Chakraborty博士表示,他们使用了NgAgo技术剪辑了海拉细胞中的相关序列,并观测到了细胞中的GFP减少的现象,这初步确认了剪辑技术发生了作用。他同时强调:“要判定韩教授的方法的可重复性,必须要等到基因测序结果出来以后才能下结论。”, beContent=null, objectType=article, channel=null, level=null, likeNumber=55, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=f0620, createdName=101.229.84.42, createdTime=Sun Jul 17 13:25:00 CST 2016, time=2016-07-17, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=93233, encodeId=ebcb93233de, content=重复出实验结果的科学家2012年发过Science,他的说服力很强。, beContent=null, objectType=article, channel=null, level=null, likeNumber=54, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=http://thirdwx.qlogo.cn/mmopen/vi_32/DYAIOgq83epgjuGZNgB6sdTz0kHXo924QwkOIy8dhZh6LQPUVR3KAwq69bLcqxSFoCAWnGUST1u00n2qAI8Gag/132, createdBy=eb581918386, createdName=五楼过客, createdTime=Sun Jul 17 11:48:00 CST 2016, time=2016-07-17, status=1, ipAttribution=)]
    2016-10-13 stfoxst
  2. [GetPortalCommentsPageByObjectIdResponse(id=2032738, encodeId=b97f2032e3873, content=<a href='/topic/show?id=ff85e343730' target=_blank style='color:#2F92EE;'>#研究者#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=25, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=73437, encryptionId=ff85e343730, topicName=研究者)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=1a19115, createdName=stfoxst, createdTime=Thu Oct 13 21:55:00 CST 2016, time=2016-10-13, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=100530, encodeId=438b100530cb, content=嗨,老方老脑筋,实验验证了?振动贴, beContent=null, objectType=article, channel=null, level=null, likeNumber=70, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=http://cacheapi.medsci.cn/resource/upload/20160601/IMG574E13FEE55F33222.jpg, createdBy=929c1478678, createdName=zibozhouping, createdTime=Thu Aug 18 20:58:00 CST 2016, time=2016-08-18, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=93361, encodeId=9b9e9336113, content=方舟子这个傻逼该被打脸了, beContent=null, objectType=article, channel=null, level=null, likeNumber=97, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=9d211716813, createdName=王抠抠, createdTime=Mon Jul 18 18:54:00 CST 2016, time=2016-07-18, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=93350, encodeId=275993350ba, content=韩教授威武!, beContent=null, objectType=article, channel=null, level=null, likeNumber=93, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=93f31631454, createdName=1dd8c52fm63(暂无匿称), createdTime=Mon Jul 18 12:22:00 CST 2016, time=2016-07-18, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1464056, encodeId=3132146405681, content=<a href='/topic/show?id=301812e562e' target=_blank style='color:#2F92EE;'>#NgAgo#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=0, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=12756, encryptionId=301812e562e, topicName=NgAgo)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=72c16575610, createdName=zhlpower, createdTime=Mon Jul 18 07:55:00 CST 2016, time=2016-07-18, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1550911, encodeId=dc8a15509116c, content=<a href='/topic/show?id=662810003e92' target=_blank style='color:#2F92EE;'>#韩春雨#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=33, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=100037, encryptionId=662810003e92, topicName=韩春雨)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=db0a14143395, createdName=bshuang, createdTime=Mon Jul 18 07:55:00 CST 2016, time=2016-07-18, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=93314, encodeId=f7a3933142f, content=这能对方舟子呵呵了,打脸, beContent=null, objectType=article, channel=null, level=null, likeNumber=90, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=http://thirdwx.qlogo.cn/mmopen/vi_32/Q0j4TwGTfTIKlpu9buib1TxXRibfFuzYXHaLxQgfUpwzYwTMo8551BS40KVZNRDLWmAXSibjfnFm0QggOrCSWElfw/132, createdBy=95aa1617853, createdName=happy1, createdTime=Mon Jul 18 07:03:00 CST 2016, time=2016-07-18, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=93241, encodeId=e9cd9324169, content=不要将一个严肃的学术问题沦落到方舟子的屁股问题. <br> 对于学术的质疑, 尤其是做过实验人得质疑, 必须严肃答复的. 当面对质疑潮时, 一个严肃的学者是必须强硬答复的. <br> Peer review的前提是文责自负, 否则哪里会有撤稿现象., beContent=null, objectType=article, channel=null, level=null, likeNumber=93, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=c4a5105539, createdName=lovetcm, createdTime=Sun Jul 17 13:26:00 CST 2016, time=2016-07-17, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=93240, encodeId=da2393240b7, content=一位来自印度基因与综合生物学研究所的Debojyoti Chakraborty博士向媒体确认“this system works”(这一系统奏效了)。Chakraborty博士表示,他们使用了NgAgo技术剪辑了海拉细胞中的相关序列,并观测到了细胞中的GFP减少的现象,这初步确认了剪辑技术发生了作用。他同时强调:“要判定韩教授的方法的可重复性,必须要等到基因测序结果出来以后才能下结论。”, beContent=null, objectType=article, channel=null, level=null, likeNumber=55, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=f0620, createdName=101.229.84.42, createdTime=Sun Jul 17 13:25:00 CST 2016, time=2016-07-17, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=93233, encodeId=ebcb93233de, content=重复出实验结果的科学家2012年发过Science,他的说服力很强。, beContent=null, objectType=article, channel=null, level=null, likeNumber=54, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=http://thirdwx.qlogo.cn/mmopen/vi_32/DYAIOgq83epgjuGZNgB6sdTz0kHXo924QwkOIy8dhZh6LQPUVR3KAwq69bLcqxSFoCAWnGUST1u00n2qAI8Gag/132, createdBy=eb581918386, createdName=五楼过客, createdTime=Sun Jul 17 11:48:00 CST 2016, time=2016-07-17, status=1, ipAttribution=)]
    2016-08-18 zibozhouping

    嗨,老方老脑筋,实验验证了?振动贴

    0

  3. [GetPortalCommentsPageByObjectIdResponse(id=2032738, encodeId=b97f2032e3873, content=<a href='/topic/show?id=ff85e343730' target=_blank style='color:#2F92EE;'>#研究者#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=25, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=73437, encryptionId=ff85e343730, topicName=研究者)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=1a19115, createdName=stfoxst, createdTime=Thu Oct 13 21:55:00 CST 2016, time=2016-10-13, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=100530, encodeId=438b100530cb, content=嗨,老方老脑筋,实验验证了?振动贴, beContent=null, objectType=article, channel=null, level=null, likeNumber=70, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=http://cacheapi.medsci.cn/resource/upload/20160601/IMG574E13FEE55F33222.jpg, createdBy=929c1478678, createdName=zibozhouping, createdTime=Thu Aug 18 20:58:00 CST 2016, time=2016-08-18, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=93361, encodeId=9b9e9336113, content=方舟子这个傻逼该被打脸了, beContent=null, objectType=article, channel=null, level=null, likeNumber=97, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=9d211716813, createdName=王抠抠, createdTime=Mon Jul 18 18:54:00 CST 2016, time=2016-07-18, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=93350, encodeId=275993350ba, content=韩教授威武!, beContent=null, objectType=article, channel=null, level=null, likeNumber=93, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=93f31631454, createdName=1dd8c52fm63(暂无匿称), createdTime=Mon Jul 18 12:22:00 CST 2016, time=2016-07-18, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1464056, encodeId=3132146405681, content=<a href='/topic/show?id=301812e562e' target=_blank style='color:#2F92EE;'>#NgAgo#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=0, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=12756, encryptionId=301812e562e, topicName=NgAgo)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=72c16575610, createdName=zhlpower, createdTime=Mon Jul 18 07:55:00 CST 2016, time=2016-07-18, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1550911, encodeId=dc8a15509116c, content=<a href='/topic/show?id=662810003e92' target=_blank style='color:#2F92EE;'>#韩春雨#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=33, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=100037, encryptionId=662810003e92, topicName=韩春雨)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=db0a14143395, createdName=bshuang, createdTime=Mon Jul 18 07:55:00 CST 2016, time=2016-07-18, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=93314, encodeId=f7a3933142f, content=这能对方舟子呵呵了,打脸, beContent=null, objectType=article, channel=null, level=null, likeNumber=90, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=http://thirdwx.qlogo.cn/mmopen/vi_32/Q0j4TwGTfTIKlpu9buib1TxXRibfFuzYXHaLxQgfUpwzYwTMo8551BS40KVZNRDLWmAXSibjfnFm0QggOrCSWElfw/132, createdBy=95aa1617853, createdName=happy1, createdTime=Mon Jul 18 07:03:00 CST 2016, time=2016-07-18, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=93241, encodeId=e9cd9324169, content=不要将一个严肃的学术问题沦落到方舟子的屁股问题. <br> 对于学术的质疑, 尤其是做过实验人得质疑, 必须严肃答复的. 当面对质疑潮时, 一个严肃的学者是必须强硬答复的. <br> Peer review的前提是文责自负, 否则哪里会有撤稿现象., beContent=null, objectType=article, channel=null, level=null, likeNumber=93, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=c4a5105539, createdName=lovetcm, createdTime=Sun Jul 17 13:26:00 CST 2016, time=2016-07-17, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=93240, encodeId=da2393240b7, content=一位来自印度基因与综合生物学研究所的Debojyoti Chakraborty博士向媒体确认“this system works”(这一系统奏效了)。Chakraborty博士表示,他们使用了NgAgo技术剪辑了海拉细胞中的相关序列,并观测到了细胞中的GFP减少的现象,这初步确认了剪辑技术发生了作用。他同时强调:“要判定韩教授的方法的可重复性,必须要等到基因测序结果出来以后才能下结论。”, beContent=null, objectType=article, channel=null, level=null, likeNumber=55, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=f0620, createdName=101.229.84.42, createdTime=Sun Jul 17 13:25:00 CST 2016, time=2016-07-17, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=93233, encodeId=ebcb93233de, content=重复出实验结果的科学家2012年发过Science,他的说服力很强。, beContent=null, objectType=article, channel=null, level=null, likeNumber=54, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=http://thirdwx.qlogo.cn/mmopen/vi_32/DYAIOgq83epgjuGZNgB6sdTz0kHXo924QwkOIy8dhZh6LQPUVR3KAwq69bLcqxSFoCAWnGUST1u00n2qAI8Gag/132, createdBy=eb581918386, createdName=五楼过客, createdTime=Sun Jul 17 11:48:00 CST 2016, time=2016-07-17, status=1, ipAttribution=)]
    2016-07-18 王抠抠

    方舟子这个傻逼该被打脸了

    0

  4. [GetPortalCommentsPageByObjectIdResponse(id=2032738, encodeId=b97f2032e3873, content=<a href='/topic/show?id=ff85e343730' target=_blank style='color:#2F92EE;'>#研究者#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=25, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=73437, encryptionId=ff85e343730, topicName=研究者)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=1a19115, createdName=stfoxst, createdTime=Thu Oct 13 21:55:00 CST 2016, time=2016-10-13, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=100530, encodeId=438b100530cb, content=嗨,老方老脑筋,实验验证了?振动贴, beContent=null, objectType=article, channel=null, level=null, likeNumber=70, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=http://cacheapi.medsci.cn/resource/upload/20160601/IMG574E13FEE55F33222.jpg, createdBy=929c1478678, createdName=zibozhouping, createdTime=Thu Aug 18 20:58:00 CST 2016, time=2016-08-18, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=93361, encodeId=9b9e9336113, content=方舟子这个傻逼该被打脸了, beContent=null, objectType=article, channel=null, level=null, likeNumber=97, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=9d211716813, createdName=王抠抠, createdTime=Mon Jul 18 18:54:00 CST 2016, time=2016-07-18, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=93350, encodeId=275993350ba, content=韩教授威武!, beContent=null, objectType=article, channel=null, level=null, likeNumber=93, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=93f31631454, createdName=1dd8c52fm63(暂无匿称), createdTime=Mon Jul 18 12:22:00 CST 2016, time=2016-07-18, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1464056, encodeId=3132146405681, content=<a href='/topic/show?id=301812e562e' target=_blank style='color:#2F92EE;'>#NgAgo#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=0, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=12756, encryptionId=301812e562e, topicName=NgAgo)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=72c16575610, createdName=zhlpower, createdTime=Mon Jul 18 07:55:00 CST 2016, time=2016-07-18, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1550911, encodeId=dc8a15509116c, content=<a href='/topic/show?id=662810003e92' target=_blank style='color:#2F92EE;'>#韩春雨#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=33, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=100037, encryptionId=662810003e92, topicName=韩春雨)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=db0a14143395, createdName=bshuang, createdTime=Mon Jul 18 07:55:00 CST 2016, time=2016-07-18, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=93314, encodeId=f7a3933142f, content=这能对方舟子呵呵了,打脸, beContent=null, objectType=article, channel=null, level=null, likeNumber=90, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=http://thirdwx.qlogo.cn/mmopen/vi_32/Q0j4TwGTfTIKlpu9buib1TxXRibfFuzYXHaLxQgfUpwzYwTMo8551BS40KVZNRDLWmAXSibjfnFm0QggOrCSWElfw/132, createdBy=95aa1617853, createdName=happy1, createdTime=Mon Jul 18 07:03:00 CST 2016, time=2016-07-18, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=93241, encodeId=e9cd9324169, content=不要将一个严肃的学术问题沦落到方舟子的屁股问题. <br> 对于学术的质疑, 尤其是做过实验人得质疑, 必须严肃答复的. 当面对质疑潮时, 一个严肃的学者是必须强硬答复的. <br> Peer review的前提是文责自负, 否则哪里会有撤稿现象., beContent=null, objectType=article, channel=null, level=null, likeNumber=93, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=c4a5105539, createdName=lovetcm, createdTime=Sun Jul 17 13:26:00 CST 2016, time=2016-07-17, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=93240, encodeId=da2393240b7, content=一位来自印度基因与综合生物学研究所的Debojyoti Chakraborty博士向媒体确认“this system works”(这一系统奏效了)。Chakraborty博士表示,他们使用了NgAgo技术剪辑了海拉细胞中的相关序列,并观测到了细胞中的GFP减少的现象,这初步确认了剪辑技术发生了作用。他同时强调:“要判定韩教授的方法的可重复性,必须要等到基因测序结果出来以后才能下结论。”, beContent=null, objectType=article, channel=null, level=null, likeNumber=55, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=f0620, createdName=101.229.84.42, createdTime=Sun Jul 17 13:25:00 CST 2016, time=2016-07-17, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=93233, encodeId=ebcb93233de, content=重复出实验结果的科学家2012年发过Science,他的说服力很强。, beContent=null, objectType=article, channel=null, level=null, likeNumber=54, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=http://thirdwx.qlogo.cn/mmopen/vi_32/DYAIOgq83epgjuGZNgB6sdTz0kHXo924QwkOIy8dhZh6LQPUVR3KAwq69bLcqxSFoCAWnGUST1u00n2qAI8Gag/132, createdBy=eb581918386, createdName=五楼过客, createdTime=Sun Jul 17 11:48:00 CST 2016, time=2016-07-17, status=1, ipAttribution=)]
    2016-07-18 1dd8c52fm63(暂无匿称)

    韩教授威武!

    0

  5. [GetPortalCommentsPageByObjectIdResponse(id=2032738, encodeId=b97f2032e3873, content=<a href='/topic/show?id=ff85e343730' target=_blank style='color:#2F92EE;'>#研究者#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=25, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=73437, encryptionId=ff85e343730, topicName=研究者)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=1a19115, createdName=stfoxst, createdTime=Thu Oct 13 21:55:00 CST 2016, time=2016-10-13, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=100530, encodeId=438b100530cb, content=嗨,老方老脑筋,实验验证了?振动贴, beContent=null, objectType=article, channel=null, level=null, likeNumber=70, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=http://cacheapi.medsci.cn/resource/upload/20160601/IMG574E13FEE55F33222.jpg, createdBy=929c1478678, createdName=zibozhouping, createdTime=Thu Aug 18 20:58:00 CST 2016, time=2016-08-18, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=93361, encodeId=9b9e9336113, content=方舟子这个傻逼该被打脸了, beContent=null, objectType=article, channel=null, level=null, likeNumber=97, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=9d211716813, createdName=王抠抠, createdTime=Mon Jul 18 18:54:00 CST 2016, time=2016-07-18, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=93350, encodeId=275993350ba, content=韩教授威武!, beContent=null, objectType=article, channel=null, level=null, likeNumber=93, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=93f31631454, createdName=1dd8c52fm63(暂无匿称), createdTime=Mon Jul 18 12:22:00 CST 2016, time=2016-07-18, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1464056, encodeId=3132146405681, content=<a href='/topic/show?id=301812e562e' target=_blank style='color:#2F92EE;'>#NgAgo#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=0, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=12756, encryptionId=301812e562e, topicName=NgAgo)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=72c16575610, createdName=zhlpower, createdTime=Mon Jul 18 07:55:00 CST 2016, time=2016-07-18, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1550911, encodeId=dc8a15509116c, content=<a href='/topic/show?id=662810003e92' target=_blank style='color:#2F92EE;'>#韩春雨#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=33, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=100037, encryptionId=662810003e92, topicName=韩春雨)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=db0a14143395, createdName=bshuang, createdTime=Mon Jul 18 07:55:00 CST 2016, time=2016-07-18, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=93314, encodeId=f7a3933142f, content=这能对方舟子呵呵了,打脸, beContent=null, objectType=article, channel=null, level=null, likeNumber=90, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=http://thirdwx.qlogo.cn/mmopen/vi_32/Q0j4TwGTfTIKlpu9buib1TxXRibfFuzYXHaLxQgfUpwzYwTMo8551BS40KVZNRDLWmAXSibjfnFm0QggOrCSWElfw/132, createdBy=95aa1617853, createdName=happy1, createdTime=Mon Jul 18 07:03:00 CST 2016, time=2016-07-18, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=93241, encodeId=e9cd9324169, content=不要将一个严肃的学术问题沦落到方舟子的屁股问题. <br> 对于学术的质疑, 尤其是做过实验人得质疑, 必须严肃答复的. 当面对质疑潮时, 一个严肃的学者是必须强硬答复的. <br> Peer review的前提是文责自负, 否则哪里会有撤稿现象., beContent=null, objectType=article, channel=null, level=null, likeNumber=93, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=c4a5105539, createdName=lovetcm, createdTime=Sun Jul 17 13:26:00 CST 2016, time=2016-07-17, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=93240, encodeId=da2393240b7, content=一位来自印度基因与综合生物学研究所的Debojyoti Chakraborty博士向媒体确认“this system works”(这一系统奏效了)。Chakraborty博士表示,他们使用了NgAgo技术剪辑了海拉细胞中的相关序列,并观测到了细胞中的GFP减少的现象,这初步确认了剪辑技术发生了作用。他同时强调:“要判定韩教授的方法的可重复性,必须要等到基因测序结果出来以后才能下结论。”, beContent=null, objectType=article, channel=null, level=null, likeNumber=55, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=f0620, createdName=101.229.84.42, createdTime=Sun Jul 17 13:25:00 CST 2016, time=2016-07-17, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=93233, encodeId=ebcb93233de, content=重复出实验结果的科学家2012年发过Science,他的说服力很强。, beContent=null, objectType=article, channel=null, level=null, likeNumber=54, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=http://thirdwx.qlogo.cn/mmopen/vi_32/DYAIOgq83epgjuGZNgB6sdTz0kHXo924QwkOIy8dhZh6LQPUVR3KAwq69bLcqxSFoCAWnGUST1u00n2qAI8Gag/132, createdBy=eb581918386, createdName=五楼过客, createdTime=Sun Jul 17 11:48:00 CST 2016, time=2016-07-17, status=1, ipAttribution=)]
    2016-07-18 zhlpower
  6. [GetPortalCommentsPageByObjectIdResponse(id=2032738, encodeId=b97f2032e3873, content=<a href='/topic/show?id=ff85e343730' target=_blank style='color:#2F92EE;'>#研究者#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=25, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=73437, encryptionId=ff85e343730, topicName=研究者)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=1a19115, createdName=stfoxst, createdTime=Thu Oct 13 21:55:00 CST 2016, time=2016-10-13, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=100530, encodeId=438b100530cb, content=嗨,老方老脑筋,实验验证了?振动贴, beContent=null, objectType=article, channel=null, level=null, likeNumber=70, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=http://cacheapi.medsci.cn/resource/upload/20160601/IMG574E13FEE55F33222.jpg, createdBy=929c1478678, createdName=zibozhouping, createdTime=Thu Aug 18 20:58:00 CST 2016, time=2016-08-18, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=93361, encodeId=9b9e9336113, content=方舟子这个傻逼该被打脸了, beContent=null, objectType=article, channel=null, level=null, likeNumber=97, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=9d211716813, createdName=王抠抠, createdTime=Mon Jul 18 18:54:00 CST 2016, time=2016-07-18, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=93350, encodeId=275993350ba, content=韩教授威武!, beContent=null, objectType=article, channel=null, level=null, likeNumber=93, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=93f31631454, createdName=1dd8c52fm63(暂无匿称), createdTime=Mon Jul 18 12:22:00 CST 2016, time=2016-07-18, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1464056, encodeId=3132146405681, content=<a href='/topic/show?id=301812e562e' target=_blank style='color:#2F92EE;'>#NgAgo#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=0, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=12756, encryptionId=301812e562e, topicName=NgAgo)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=72c16575610, createdName=zhlpower, createdTime=Mon Jul 18 07:55:00 CST 2016, time=2016-07-18, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1550911, encodeId=dc8a15509116c, content=<a href='/topic/show?id=662810003e92' target=_blank style='color:#2F92EE;'>#韩春雨#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=33, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=100037, encryptionId=662810003e92, topicName=韩春雨)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=db0a14143395, createdName=bshuang, createdTime=Mon Jul 18 07:55:00 CST 2016, time=2016-07-18, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=93314, encodeId=f7a3933142f, content=这能对方舟子呵呵了,打脸, beContent=null, objectType=article, channel=null, level=null, likeNumber=90, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=http://thirdwx.qlogo.cn/mmopen/vi_32/Q0j4TwGTfTIKlpu9buib1TxXRibfFuzYXHaLxQgfUpwzYwTMo8551BS40KVZNRDLWmAXSibjfnFm0QggOrCSWElfw/132, createdBy=95aa1617853, createdName=happy1, createdTime=Mon Jul 18 07:03:00 CST 2016, time=2016-07-18, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=93241, encodeId=e9cd9324169, content=不要将一个严肃的学术问题沦落到方舟子的屁股问题. <br> 对于学术的质疑, 尤其是做过实验人得质疑, 必须严肃答复的. 当面对质疑潮时, 一个严肃的学者是必须强硬答复的. <br> Peer review的前提是文责自负, 否则哪里会有撤稿现象., beContent=null, objectType=article, channel=null, level=null, likeNumber=93, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=c4a5105539, createdName=lovetcm, createdTime=Sun Jul 17 13:26:00 CST 2016, time=2016-07-17, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=93240, encodeId=da2393240b7, content=一位来自印度基因与综合生物学研究所的Debojyoti Chakraborty博士向媒体确认“this system works”(这一系统奏效了)。Chakraborty博士表示,他们使用了NgAgo技术剪辑了海拉细胞中的相关序列,并观测到了细胞中的GFP减少的现象,这初步确认了剪辑技术发生了作用。他同时强调:“要判定韩教授的方法的可重复性,必须要等到基因测序结果出来以后才能下结论。”, beContent=null, objectType=article, channel=null, level=null, likeNumber=55, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=f0620, createdName=101.229.84.42, createdTime=Sun Jul 17 13:25:00 CST 2016, time=2016-07-17, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=93233, encodeId=ebcb93233de, content=重复出实验结果的科学家2012年发过Science,他的说服力很强。, beContent=null, objectType=article, channel=null, level=null, likeNumber=54, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=http://thirdwx.qlogo.cn/mmopen/vi_32/DYAIOgq83epgjuGZNgB6sdTz0kHXo924QwkOIy8dhZh6LQPUVR3KAwq69bLcqxSFoCAWnGUST1u00n2qAI8Gag/132, createdBy=eb581918386, createdName=五楼过客, createdTime=Sun Jul 17 11:48:00 CST 2016, time=2016-07-17, status=1, ipAttribution=)]
    2016-07-18 bshuang
  7. [GetPortalCommentsPageByObjectIdResponse(id=2032738, encodeId=b97f2032e3873, content=<a href='/topic/show?id=ff85e343730' target=_blank style='color:#2F92EE;'>#研究者#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=25, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=73437, encryptionId=ff85e343730, topicName=研究者)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=1a19115, createdName=stfoxst, createdTime=Thu Oct 13 21:55:00 CST 2016, time=2016-10-13, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=100530, encodeId=438b100530cb, content=嗨,老方老脑筋,实验验证了?振动贴, beContent=null, objectType=article, channel=null, level=null, likeNumber=70, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=http://cacheapi.medsci.cn/resource/upload/20160601/IMG574E13FEE55F33222.jpg, createdBy=929c1478678, createdName=zibozhouping, createdTime=Thu Aug 18 20:58:00 CST 2016, time=2016-08-18, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=93361, encodeId=9b9e9336113, content=方舟子这个傻逼该被打脸了, beContent=null, objectType=article, channel=null, level=null, likeNumber=97, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=9d211716813, createdName=王抠抠, createdTime=Mon Jul 18 18:54:00 CST 2016, time=2016-07-18, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=93350, encodeId=275993350ba, content=韩教授威武!, beContent=null, objectType=article, channel=null, level=null, likeNumber=93, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=93f31631454, createdName=1dd8c52fm63(暂无匿称), createdTime=Mon Jul 18 12:22:00 CST 2016, time=2016-07-18, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1464056, encodeId=3132146405681, content=<a href='/topic/show?id=301812e562e' target=_blank style='color:#2F92EE;'>#NgAgo#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=0, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=12756, encryptionId=301812e562e, topicName=NgAgo)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=72c16575610, createdName=zhlpower, createdTime=Mon Jul 18 07:55:00 CST 2016, time=2016-07-18, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1550911, encodeId=dc8a15509116c, content=<a href='/topic/show?id=662810003e92' target=_blank style='color:#2F92EE;'>#韩春雨#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=33, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=100037, encryptionId=662810003e92, topicName=韩春雨)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=db0a14143395, createdName=bshuang, createdTime=Mon Jul 18 07:55:00 CST 2016, time=2016-07-18, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=93314, encodeId=f7a3933142f, content=这能对方舟子呵呵了,打脸, beContent=null, objectType=article, channel=null, level=null, likeNumber=90, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=http://thirdwx.qlogo.cn/mmopen/vi_32/Q0j4TwGTfTIKlpu9buib1TxXRibfFuzYXHaLxQgfUpwzYwTMo8551BS40KVZNRDLWmAXSibjfnFm0QggOrCSWElfw/132, createdBy=95aa1617853, createdName=happy1, createdTime=Mon Jul 18 07:03:00 CST 2016, time=2016-07-18, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=93241, encodeId=e9cd9324169, content=不要将一个严肃的学术问题沦落到方舟子的屁股问题. <br> 对于学术的质疑, 尤其是做过实验人得质疑, 必须严肃答复的. 当面对质疑潮时, 一个严肃的学者是必须强硬答复的. <br> Peer review的前提是文责自负, 否则哪里会有撤稿现象., beContent=null, objectType=article, channel=null, level=null, likeNumber=93, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=c4a5105539, createdName=lovetcm, createdTime=Sun Jul 17 13:26:00 CST 2016, time=2016-07-17, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=93240, encodeId=da2393240b7, content=一位来自印度基因与综合生物学研究所的Debojyoti Chakraborty博士向媒体确认“this system works”(这一系统奏效了)。Chakraborty博士表示,他们使用了NgAgo技术剪辑了海拉细胞中的相关序列,并观测到了细胞中的GFP减少的现象,这初步确认了剪辑技术发生了作用。他同时强调:“要判定韩教授的方法的可重复性,必须要等到基因测序结果出来以后才能下结论。”, beContent=null, objectType=article, channel=null, level=null, likeNumber=55, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=f0620, createdName=101.229.84.42, createdTime=Sun Jul 17 13:25:00 CST 2016, time=2016-07-17, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=93233, encodeId=ebcb93233de, content=重复出实验结果的科学家2012年发过Science,他的说服力很强。, beContent=null, objectType=article, channel=null, level=null, likeNumber=54, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=http://thirdwx.qlogo.cn/mmopen/vi_32/DYAIOgq83epgjuGZNgB6sdTz0kHXo924QwkOIy8dhZh6LQPUVR3KAwq69bLcqxSFoCAWnGUST1u00n2qAI8Gag/132, createdBy=eb581918386, createdName=五楼过客, createdTime=Sun Jul 17 11:48:00 CST 2016, time=2016-07-17, status=1, ipAttribution=)]
    2016-07-18 happy1

    这能对方舟子呵呵了,打脸

    0

  8. [GetPortalCommentsPageByObjectIdResponse(id=2032738, encodeId=b97f2032e3873, content=<a href='/topic/show?id=ff85e343730' target=_blank style='color:#2F92EE;'>#研究者#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=25, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=73437, encryptionId=ff85e343730, topicName=研究者)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=1a19115, createdName=stfoxst, createdTime=Thu Oct 13 21:55:00 CST 2016, time=2016-10-13, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=100530, encodeId=438b100530cb, content=嗨,老方老脑筋,实验验证了?振动贴, beContent=null, objectType=article, channel=null, level=null, likeNumber=70, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=http://cacheapi.medsci.cn/resource/upload/20160601/IMG574E13FEE55F33222.jpg, createdBy=929c1478678, createdName=zibozhouping, createdTime=Thu Aug 18 20:58:00 CST 2016, time=2016-08-18, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=93361, encodeId=9b9e9336113, content=方舟子这个傻逼该被打脸了, beContent=null, objectType=article, channel=null, level=null, likeNumber=97, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=9d211716813, createdName=王抠抠, createdTime=Mon Jul 18 18:54:00 CST 2016, time=2016-07-18, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=93350, encodeId=275993350ba, content=韩教授威武!, beContent=null, objectType=article, channel=null, level=null, likeNumber=93, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=93f31631454, createdName=1dd8c52fm63(暂无匿称), createdTime=Mon Jul 18 12:22:00 CST 2016, time=2016-07-18, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1464056, encodeId=3132146405681, content=<a href='/topic/show?id=301812e562e' target=_blank style='color:#2F92EE;'>#NgAgo#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=0, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=12756, encryptionId=301812e562e, topicName=NgAgo)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=72c16575610, createdName=zhlpower, createdTime=Mon Jul 18 07:55:00 CST 2016, time=2016-07-18, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1550911, encodeId=dc8a15509116c, content=<a href='/topic/show?id=662810003e92' target=_blank style='color:#2F92EE;'>#韩春雨#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=33, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=100037, encryptionId=662810003e92, topicName=韩春雨)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=db0a14143395, createdName=bshuang, createdTime=Mon Jul 18 07:55:00 CST 2016, time=2016-07-18, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=93314, encodeId=f7a3933142f, content=这能对方舟子呵呵了,打脸, beContent=null, objectType=article, channel=null, level=null, likeNumber=90, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=http://thirdwx.qlogo.cn/mmopen/vi_32/Q0j4TwGTfTIKlpu9buib1TxXRibfFuzYXHaLxQgfUpwzYwTMo8551BS40KVZNRDLWmAXSibjfnFm0QggOrCSWElfw/132, createdBy=95aa1617853, createdName=happy1, createdTime=Mon Jul 18 07:03:00 CST 2016, time=2016-07-18, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=93241, encodeId=e9cd9324169, content=不要将一个严肃的学术问题沦落到方舟子的屁股问题. <br> 对于学术的质疑, 尤其是做过实验人得质疑, 必须严肃答复的. 当面对质疑潮时, 一个严肃的学者是必须强硬答复的. <br> Peer review的前提是文责自负, 否则哪里会有撤稿现象., beContent=null, objectType=article, channel=null, level=null, likeNumber=93, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=c4a5105539, createdName=lovetcm, createdTime=Sun Jul 17 13:26:00 CST 2016, time=2016-07-17, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=93240, encodeId=da2393240b7, content=一位来自印度基因与综合生物学研究所的Debojyoti Chakraborty博士向媒体确认“this system works”(这一系统奏效了)。Chakraborty博士表示,他们使用了NgAgo技术剪辑了海拉细胞中的相关序列,并观测到了细胞中的GFP减少的现象,这初步确认了剪辑技术发生了作用。他同时强调:“要判定韩教授的方法的可重复性,必须要等到基因测序结果出来以后才能下结论。”, beContent=null, objectType=article, channel=null, level=null, likeNumber=55, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=f0620, createdName=101.229.84.42, createdTime=Sun Jul 17 13:25:00 CST 2016, time=2016-07-17, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=93233, encodeId=ebcb93233de, content=重复出实验结果的科学家2012年发过Science,他的说服力很强。, beContent=null, objectType=article, channel=null, level=null, likeNumber=54, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=http://thirdwx.qlogo.cn/mmopen/vi_32/DYAIOgq83epgjuGZNgB6sdTz0kHXo924QwkOIy8dhZh6LQPUVR3KAwq69bLcqxSFoCAWnGUST1u00n2qAI8Gag/132, createdBy=eb581918386, createdName=五楼过客, createdTime=Sun Jul 17 11:48:00 CST 2016, time=2016-07-17, status=1, ipAttribution=)]
    2016-07-17 lovetcm

    不要将一个严肃的学术问题沦落到方舟子的屁股问题.
    对于学术的质疑, 尤其是做过实验人得质疑, 必须严肃答复的. 当面对质疑潮时, 一个严肃的学者是必须强硬答复的.
    Peer review的前提是文责自负, 否则哪里会有撤稿现象.

    0

  9. [GetPortalCommentsPageByObjectIdResponse(id=2032738, encodeId=b97f2032e3873, content=<a href='/topic/show?id=ff85e343730' target=_blank style='color:#2F92EE;'>#研究者#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=25, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=73437, encryptionId=ff85e343730, topicName=研究者)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=1a19115, createdName=stfoxst, createdTime=Thu Oct 13 21:55:00 CST 2016, time=2016-10-13, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=100530, encodeId=438b100530cb, content=嗨,老方老脑筋,实验验证了?振动贴, beContent=null, objectType=article, channel=null, level=null, likeNumber=70, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=http://cacheapi.medsci.cn/resource/upload/20160601/IMG574E13FEE55F33222.jpg, createdBy=929c1478678, createdName=zibozhouping, createdTime=Thu Aug 18 20:58:00 CST 2016, time=2016-08-18, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=93361, encodeId=9b9e9336113, content=方舟子这个傻逼该被打脸了, beContent=null, objectType=article, channel=null, level=null, likeNumber=97, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=9d211716813, createdName=王抠抠, createdTime=Mon Jul 18 18:54:00 CST 2016, time=2016-07-18, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=93350, encodeId=275993350ba, content=韩教授威武!, beContent=null, objectType=article, channel=null, level=null, likeNumber=93, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=93f31631454, createdName=1dd8c52fm63(暂无匿称), createdTime=Mon Jul 18 12:22:00 CST 2016, time=2016-07-18, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1464056, encodeId=3132146405681, content=<a href='/topic/show?id=301812e562e' target=_blank style='color:#2F92EE;'>#NgAgo#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=0, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=12756, encryptionId=301812e562e, topicName=NgAgo)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=72c16575610, createdName=zhlpower, createdTime=Mon Jul 18 07:55:00 CST 2016, time=2016-07-18, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1550911, encodeId=dc8a15509116c, content=<a href='/topic/show?id=662810003e92' target=_blank style='color:#2F92EE;'>#韩春雨#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=33, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=100037, encryptionId=662810003e92, topicName=韩春雨)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=db0a14143395, createdName=bshuang, createdTime=Mon Jul 18 07:55:00 CST 2016, time=2016-07-18, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=93314, encodeId=f7a3933142f, content=这能对方舟子呵呵了,打脸, beContent=null, objectType=article, channel=null, level=null, likeNumber=90, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=http://thirdwx.qlogo.cn/mmopen/vi_32/Q0j4TwGTfTIKlpu9buib1TxXRibfFuzYXHaLxQgfUpwzYwTMo8551BS40KVZNRDLWmAXSibjfnFm0QggOrCSWElfw/132, createdBy=95aa1617853, createdName=happy1, createdTime=Mon Jul 18 07:03:00 CST 2016, time=2016-07-18, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=93241, encodeId=e9cd9324169, content=不要将一个严肃的学术问题沦落到方舟子的屁股问题. <br> 对于学术的质疑, 尤其是做过实验人得质疑, 必须严肃答复的. 当面对质疑潮时, 一个严肃的学者是必须强硬答复的. <br> Peer review的前提是文责自负, 否则哪里会有撤稿现象., beContent=null, objectType=article, channel=null, level=null, likeNumber=93, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=c4a5105539, createdName=lovetcm, createdTime=Sun Jul 17 13:26:00 CST 2016, time=2016-07-17, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=93240, encodeId=da2393240b7, content=一位来自印度基因与综合生物学研究所的Debojyoti Chakraborty博士向媒体确认“this system works”(这一系统奏效了)。Chakraborty博士表示,他们使用了NgAgo技术剪辑了海拉细胞中的相关序列,并观测到了细胞中的GFP减少的现象,这初步确认了剪辑技术发生了作用。他同时强调:“要判定韩教授的方法的可重复性,必须要等到基因测序结果出来以后才能下结论。”, beContent=null, objectType=article, channel=null, level=null, likeNumber=55, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=f0620, createdName=101.229.84.42, createdTime=Sun Jul 17 13:25:00 CST 2016, time=2016-07-17, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=93233, encodeId=ebcb93233de, content=重复出实验结果的科学家2012年发过Science,他的说服力很强。, beContent=null, objectType=article, channel=null, level=null, likeNumber=54, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=http://thirdwx.qlogo.cn/mmopen/vi_32/DYAIOgq83epgjuGZNgB6sdTz0kHXo924QwkOIy8dhZh6LQPUVR3KAwq69bLcqxSFoCAWnGUST1u00n2qAI8Gag/132, createdBy=eb581918386, createdName=五楼过客, createdTime=Sun Jul 17 11:48:00 CST 2016, time=2016-07-17, status=1, ipAttribution=)]
    2016-07-17 101.229.84.42

    一位来自印度基因与综合生物学研究所的Debojyoti Chakraborty博士向媒体确认“this system works”(这一系统奏效了)。Chakraborty博士表示,他们使用了NgAgo技术剪辑了海拉细胞中的相关序列,并观测到了细胞中的GFP减少的现象,这初步确认了剪辑技术发生了作用。他同时强调:“要判定韩教授的方法的可重复性,必须要等到基因测序结果出来以后才能下结论。”

    0

  10. [GetPortalCommentsPageByObjectIdResponse(id=2032738, encodeId=b97f2032e3873, content=<a href='/topic/show?id=ff85e343730' target=_blank style='color:#2F92EE;'>#研究者#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=25, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=73437, encryptionId=ff85e343730, topicName=研究者)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=1a19115, createdName=stfoxst, createdTime=Thu Oct 13 21:55:00 CST 2016, time=2016-10-13, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=100530, encodeId=438b100530cb, content=嗨,老方老脑筋,实验验证了?振动贴, beContent=null, objectType=article, channel=null, level=null, likeNumber=70, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=http://cacheapi.medsci.cn/resource/upload/20160601/IMG574E13FEE55F33222.jpg, createdBy=929c1478678, createdName=zibozhouping, createdTime=Thu Aug 18 20:58:00 CST 2016, time=2016-08-18, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=93361, encodeId=9b9e9336113, content=方舟子这个傻逼该被打脸了, beContent=null, objectType=article, channel=null, level=null, likeNumber=97, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=9d211716813, createdName=王抠抠, createdTime=Mon Jul 18 18:54:00 CST 2016, time=2016-07-18, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=93350, encodeId=275993350ba, content=韩教授威武!, beContent=null, objectType=article, channel=null, level=null, likeNumber=93, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=93f31631454, createdName=1dd8c52fm63(暂无匿称), createdTime=Mon Jul 18 12:22:00 CST 2016, time=2016-07-18, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1464056, encodeId=3132146405681, content=<a href='/topic/show?id=301812e562e' target=_blank style='color:#2F92EE;'>#NgAgo#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=0, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=12756, encryptionId=301812e562e, topicName=NgAgo)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=72c16575610, createdName=zhlpower, createdTime=Mon Jul 18 07:55:00 CST 2016, time=2016-07-18, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1550911, encodeId=dc8a15509116c, content=<a href='/topic/show?id=662810003e92' target=_blank style='color:#2F92EE;'>#韩春雨#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=33, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=100037, encryptionId=662810003e92, topicName=韩春雨)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=db0a14143395, createdName=bshuang, createdTime=Mon Jul 18 07:55:00 CST 2016, time=2016-07-18, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=93314, encodeId=f7a3933142f, content=这能对方舟子呵呵了,打脸, beContent=null, objectType=article, channel=null, level=null, likeNumber=90, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=http://thirdwx.qlogo.cn/mmopen/vi_32/Q0j4TwGTfTIKlpu9buib1TxXRibfFuzYXHaLxQgfUpwzYwTMo8551BS40KVZNRDLWmAXSibjfnFm0QggOrCSWElfw/132, createdBy=95aa1617853, createdName=happy1, createdTime=Mon Jul 18 07:03:00 CST 2016, time=2016-07-18, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=93241, encodeId=e9cd9324169, content=不要将一个严肃的学术问题沦落到方舟子的屁股问题. <br> 对于学术的质疑, 尤其是做过实验人得质疑, 必须严肃答复的. 当面对质疑潮时, 一个严肃的学者是必须强硬答复的. <br> Peer review的前提是文责自负, 否则哪里会有撤稿现象., beContent=null, objectType=article, channel=null, level=null, likeNumber=93, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=c4a5105539, createdName=lovetcm, createdTime=Sun Jul 17 13:26:00 CST 2016, time=2016-07-17, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=93240, encodeId=da2393240b7, content=一位来自印度基因与综合生物学研究所的Debojyoti Chakraborty博士向媒体确认“this system works”(这一系统奏效了)。Chakraborty博士表示,他们使用了NgAgo技术剪辑了海拉细胞中的相关序列,并观测到了细胞中的GFP减少的现象,这初步确认了剪辑技术发生了作用。他同时强调:“要判定韩教授的方法的可重复性,必须要等到基因测序结果出来以后才能下结论。”, beContent=null, objectType=article, channel=null, level=null, likeNumber=55, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=f0620, createdName=101.229.84.42, createdTime=Sun Jul 17 13:25:00 CST 2016, time=2016-07-17, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=93233, encodeId=ebcb93233de, content=重复出实验结果的科学家2012年发过Science,他的说服力很强。, beContent=null, objectType=article, channel=null, level=null, likeNumber=54, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=http://thirdwx.qlogo.cn/mmopen/vi_32/DYAIOgq83epgjuGZNgB6sdTz0kHXo924QwkOIy8dhZh6LQPUVR3KAwq69bLcqxSFoCAWnGUST1u00n2qAI8Gag/132, createdBy=eb581918386, createdName=五楼过客, createdTime=Sun Jul 17 11:48:00 CST 2016, time=2016-07-17, status=1, ipAttribution=)]
    2016-07-17 五楼过客

    重复出实验结果的科学家2012年发过Science,他的说服力很强。

    0

相关资讯

临床上搞了点小发明,然后怎么办?——从韩春雨诺奖级项目说起

导语:知识产权日益成为国家发展的战略性资源和核心要素,专利制度在激励创新、推动经济发展和社会进步中的支撑作用日益凸显。近几天,韩春雨副教授在NatureBiotech发表重大原创成果的报道抢占了很多媒体的头条,让众人称赞。很多研究人员认为韩春雨报道的NgAgo-gDNA技术更适合人类基因组编辑,将有可能超越CRISPR-Cas9技术。科学网博客作者————沙晨检索了韩春雨的专利,发现了一些问题

方舟子发文质疑韩春雨“诺奖级”实验成果,你怎么看?

说起中国目前最炽手可热的生物科学家,大家脑海里第一个跳出来的名字十有八九是韩春雨,除却研究的重大意义,韩春雨研究环境的简单、经费紧张已成为大家津津乐道的励志典范。但近日,方舟子公开发文对韩春雨的“诺奖级”论文的“实验的可重复性”。 以下为方舟子《河北科技大学韩春雨“诺贝尔奖级”实验的重复性问题》的部分节选: 这几天我陆续收到几家实验室的研究人员的来信,反映重复不出韩春雨论文中最关键的图

Nat Biotech:中国学者开发出替代CAS9的新型基因编辑技术,诺奖级别

5月2日,Nature系列顶级刊物Nature Biotechnology在线发表了来自河北科技大学生物科学与工程学院青年教师韩春雨副教授题为“DNA-guided genome editing using the Natronobacterium gregoryi Argonaute”的重大原创性成果。这项全新的基因编辑技术对CAS9技术提出重要的挑战,它几乎规避了CAS

没有火之前的韩春雨教授,真实,霸气侧漏!

韩春雨教授因为发现新型基因编辑系统,被称为中国最有希望获得诺贝尔奖的科学家之一!另外一个关键在于,他在河北科技大学(估计很多人没有听过,其实小编以前也没有听过!),一所三流学校(以后会不会因为韩春雨进入二流,甚至一流学校?记得以前中国有一个奇葩大学---西北大学,每年大学排行榜,都进入不了前100名,不过,每次下方都会有这么一句话:虽然西北大学无法进入前100名,但是,去年舒德干教授又发表了2篇N

央视“点赞”韩春雨!“魔剪CRISPR”新近进展TOP7

自河北科技大学副教授韩春雨团队的基因编辑成果发表以来,受到了科学界和媒体的广泛关注。6月16日,央视报道称,目前最时兴的是第三代基因编辑技术,近些年来一直是诺贝尔奖的热门。韩春雨团队发明的新基因编辑工具在很多人看来是对第三代技术的挑战,也被称为第四代基因编辑技术。报道还表示,韩春雨团队的发现能有效针对基因组内的任何位置进行定位并改造,对游离于细胞核内的DNA具有更高的切割效率,大幅降低了基因编