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Clinica Chimica Acta：基于流动注射电离-串联质谱法对干血滴中一组溶血磷脂酰胆碱的检测可用于x -连锁肾上腺脑营养不良的筛查

2019-09-21 不详 MedSci原创

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众所周知，C26:0溶血磷脂酰胆碱(LPC)升高是x -肾上腺脑白质营养不良(X-ALD)的诊断指标。本研究的目的是开发一种流动注射电离串联质谱(FIA-MS/MS)方法来估计干血滴(DBS)中的LPCs (C20:0-C26:0-LPCs)，并确定该方法用于高通量筛查X-ALD的敏感性和特异性。

众所周知，C26:0溶血磷脂酰胆碱(LPC)升高是x -肾上腺脑白质营养不良(X-ALD)的诊断指标。本研究的目的是开发一种流动注射电离串联质谱(FIA-MS/MS)方法来估计干血滴(DBS)中的LPCs (C20:0-C26:0-LPCs)，并确定该方法用于高通量筛查X-ALD的敏感性和特异性。

研究人员在一个96孔从3.2毫米DBS板萃取LPCS（C20：0-C26：0），并进行同位素标记的内标（C26：0-D4-LPC），采用三重四极串联质谱仪在FIA-MS / MS电喷雾离子化测得的（ESI）阳性，多反应监测（MRM）模式检测。同时确定FIA-MS / MS方法对X-ALD的筛查的灵敏度和特异性测定，并对FIA-MS / MS方法与LC-MS / MS方法用于估计LPC浓度进行比较

研究显示C26:0和C24:0-LPCs升高对X-ALD的鉴别具有100%的敏感性。然而，C26:0和C24:0-LPCs的特异性仅为78.33%和98.33%。C22:0和C20:0 LPCs的敏感性分别为89.29%、78.33%和特异性分别为67.86%和73.33%。FIA-MS/MS方法与LC-MS/MS方法具有良好的一致性。

研究表明，用FIA-MS/MS方法估计DBS中C26:0和C24:0-LPCs适用于新生儿X-ALD的一级筛查。筛查阳性病例需要二级验证性试验。

原始出处：

Archana Natarajan，Rita Christopher，Flow injection ionization-tandem mass spectrometry-based estimation of a panel of lysophosphatidylcholines in dried blood spots for screening of X-linked adrenoleukodystrophy

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    2019-11-18 xuyong523

    #肾上腺#

    0

  2. [GetPortalCommentsPageByObjectIdResponse(id=1895188, encodeId=b5391895188e9, content=<a href='/topic/show?id=ce478241e0b' target=_blank style='color:#2F92EE;'>#肾上腺#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=32, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=82417, encryptionId=ce478241e0b, topicName=肾上腺)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=8dba135, createdName=xuyong523, createdTime=Mon Nov 18 15:13:00 CST 2019, time=2019-11-18, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1849725, encodeId=5a661849e2508, content=<a href='/topic/show?id=61815385aa' target=_blank style='color:#2F92EE;'>#CTA#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=39, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=5385, encryptionId=61815385aa, topicName=CTA)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=ec9955, createdName=windight, createdTime=Sat May 23 13:13:00 CST 2020, time=2020-05-23, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1527415, encodeId=428f152e41511, content=<a href='/topic/show?id=10b483495a2' target=_blank style='color:#2F92EE;'>#胆碱#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=36, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=83495, encryptionId=10b483495a2, topicName=胆碱)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=ce6211859522, createdName=grace5704, createdTime=Mon Sep 23 14:13:00 CST 2019, time=2019-09-23, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1553362, encodeId=a7611553362eb, content=<a href='/topic/show?id=81bd923e559' target=_blank style='color:#2F92EE;'>#质谱#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=33, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=92375, encryptionId=81bd923e559, topicName=质谱)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=96fd14366106, createdName=jeanqiuqiu, createdTime=Mon Sep 23 14:13:00 CST 2019, time=2019-09-23, status=1, ipAttribution=)]
    2020-05-23 windight

    #CTA#

    0

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    2019-09-23 grace5704

    #胆碱#

    0

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    2019-09-23 jeanqiuqiu

    #质谱#

    0

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