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Biomed Res Int:使用新型细胞穿透肽R9-LK15递送miRNA-29b促进骨髓间充质干细胞的成骨分化

2019-05-25 不详 网络

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促进成骨的微小RNA（miRNA）的递送是增强骨再生的有前途的方法。在本研究中，研究人员制备了包含新型细胞穿透肽R9-LK15和miR-29b的纳米复合物，并研究了它们对骨髓间充质干细胞（BMSCs）成骨分化的影响。制备并表征了R9-LK15 / miR-29b纳米复合物。研究了转染效率，细胞活力和成骨分化。结果显示，R9-LK15维持血清中miR-29b的稳定性长达24小时。此外，R9-LK15

促进成骨的微小RNA（miRNA）的递送是增强骨再生的有前途的方法。在本研究中，研究人员制备了包含新型细胞穿透肽R9-LK15和miR-29b的纳米复合物，并研究了它们对骨髓间充质干细胞（BMSCs）成骨分化的影响。

制备并表征了R9-LK15 / miR-29b纳米复合物。研究了转染效率，细胞活力和成骨分化。结果显示，R9-LK15维持血清中miR-29b的稳定性长达24小时。此外，R9-LK15有效地将miR-29b递送到BMSC中;转染效率比使用Lipofectamine 2000获得的转染效率高约10倍。细胞计数试剂盒-8测定显示R9-LK15和R9-LK15/miR-29b纳米复合物对BMSCs的细胞毒性作用可忽略不计。R9-LK15/miR-29b纳米复合物的递送通过上调碱性磷酸酶表达和下调组蛋​​白脱乙酰酶-4表达促进BMSCs的成骨分化和细胞外基质矿化。

总体而言，我们开发了一种新型miRNA传递系统，其具有高转染效率并促进成骨分化。

原始出处：

Liu Q, Lin Z,  et al., Delivery of miRNA-29b Using R9-LK15, a Novel Cell-Penetrating Peptide, Promotes Osteogenic Differentiation of Bone Mesenchymal Stem Cells. Biomed Res Int. 2019 Apr 11;2019:3032158. doi: 10.1155/2019/3032158. eCollection 2019.

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    2019-06-10 sunylz

    #Bio#

    0

  2. 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    2020-02-25 smallant2002

    #miR#

    0

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    2019-12-06 h.y200607_23674418

    #间充质干细#

    0

  4. 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    2019-08-04 zhouqu_8

    #骨分化#

    0

  5. 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    2019-12-13 ying_wu

    #骨髓间充质干细胞#

    0

  6. 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createdBy=e87d405, createdName=Homburg, createdTime=Mon May 27 04:17:00 CST 2019, time=2019-05-27, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1337574, encodeId=a06c133e57438, content=<a href='/topic/show?id=863f5409610' target=_blank style='color:#2F92EE;'>#成骨分化#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=26, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=54096, encryptionId=863f5409610, topicName=成骨分化)], attachment=null, authenticateStatus=null, createdAvatar=http://thirdwx.qlogo.cn/mmopen/vi_32/DYAIOgq83eqySEUYKeYDjXJMYMrtscBNkpAnLfQZgDcdkEratcJPqS7ARJOXqllZQhCSFicErlBFbIywuoiaP0LQ/132, createdBy=012d2500132, createdName=ms5880526374914242, createdTime=Mon May 27 04:17:00 CST 2019, time=2019-05-27, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1352145, encodeId=5bf7135214578, content=<a href='/topic/show?id=e95954093cd' target=_blank style='color:#2F92EE;'>#成骨#</a>, 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    2019-12-23 neurowu

    #miRNA-29#

    0

  7. 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createdBy=e87d405, createdName=Homburg, createdTime=Mon May 27 04:17:00 CST 2019, time=2019-05-27, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1337574, encodeId=a06c133e57438, content=<a href='/topic/show?id=863f5409610' target=_blank style='color:#2F92EE;'>#成骨分化#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=26, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=54096, encryptionId=863f5409610, topicName=成骨分化)], attachment=null, authenticateStatus=null, createdAvatar=http://thirdwx.qlogo.cn/mmopen/vi_32/DYAIOgq83eqySEUYKeYDjXJMYMrtscBNkpAnLfQZgDcdkEratcJPqS7ARJOXqllZQhCSFicErlBFbIywuoiaP0LQ/132, createdBy=012d2500132, createdName=ms5880526374914242, createdTime=Mon May 27 04:17:00 CST 2019, time=2019-05-27, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1352145, encodeId=5bf7135214578, content=<a href='/topic/show?id=e95954093cd' target=_blank style='color:#2F92EE;'>#成骨#</a>, 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    2019-05-27 Homburg

    #miRNA#

    0

  8. 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    2019-05-27 ms5880526374914242

    #成骨分化#

    0

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    2019-05-27 zhaojie88

    #成骨#

    0

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    2019-05-27 ms3994565386320060

    #Med#

    0

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