骨科

订阅

MedSci主页 / 所有栏目 / 骨科

IUBMB Life:HAMSCs / HBMSCs transwell和混合共培养系统的成骨效果对比

2019-05-25 不详 网络

关注

们先前的研究表明，含有人羊膜来源的间充质干细胞（HAMSCs）和人骨髓间充质干细胞（HBMSCs）的共培养系统具有应用于骨再生的潜力。然而，目前在HAMSCs/HBMSCs transwell和混合共培养系统之间尚无足够的比较研究。本研究旨在分别评估间接和直接共培养系统调控的表型和机制。两种体外模型与HAMSCs和HBMSCs以3：1的比例一起使用，然后通过一系列过程进行分析，包括流式细胞术，碱性

我们先前的研究表明，含有人羊膜来源的间充质干细胞（HAMSCs）和人骨髓间充质干细胞（HBMSCs）的共培养系统具有应用于骨再生的潜力。然而，目前在HAMSCs/HBMSCs transwell和混合共培养系统之间尚无足够的比较研究。本研究旨在分别评估间接和直接共培养系统调控的表型和机制。

两种体外模型与HAMSCs和HBMSCs以3：1的比例一起使用，然后通过一系列过程进行分析，包括流式细胞术，碱性磷酸酶（ALP）底物测定，茜素红S染色，定量逆转录聚合酶链反应（RT-qPCR）和Western印迹分析。

我们发现，与混合共培养系统相比，transwell共培养系统中细胞增殖，ALP活性，矿化基质形成和成骨细胞相关mRNA表达加速。来自transwell共培养系统的条件培养基实现了血管内皮生长因子水平的升高，并诱导了人脐静脉内皮细胞中比混合共培养系统更多的血管结构。此外，我们观察到transwell共培养系统，通过细胞外调节蛋白激酶1/2（ERK1/2）丝裂原活化蛋白激酶（MAPK）信号通路维持干细胞，促进成骨和血管生成。U0126是ERK1 / 2 MAPK信号传导的选择性抑制剂，明显抑制了对transwell共培养系统的基于干性的影响的维持。

总之，我们的结果比较了两种不同模型的优点，并阐明了HAMSCs / HBMSCs transwell共培养系统在骨组织工程开发中的作用。

原始出处：

Bian Y, Du Y, et al., A comparative study of HAMSCs/HBMSCs transwell and mixed coculture systems. IUBMB Life. 2019 May 21. doi: 10.1002/iub.2074. 

版权声明：
本网站所有内容来源注明为“梅斯医学”或“MedSci原创”的文字、图片和音视频资料，版权均属于梅斯医学所有。非经授权，任何媒体、网站或个人不得转载，授权转载时须注明来源为“梅斯医学”。其它来源的文章系转载文章，或“梅斯号”自媒体发布的文章，仅系出于传递更多信息之目的，本站仅负责审核内容合规，其内容不代表本站立场，本站不负责内容的准确性和版权。如果存在侵权、或不希望被转载的媒体或个人可与我们联系，我们将立即进行删除处理。
在此留言
评论区 (8)
#插入话题
  1. [GetPortalCommentsPageByObjectIdResponse(id=2068593, encodeId=7a36206859376, content=<a href='/topic/show?id=59371088215' target=_blank style='color:#2F92EE;'>#Life#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=27, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=10882, encryptionId=59371088215, topicName=Life)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=fc8528, createdName=clmlylxy, createdTime=Fri Apr 10 16:47:00 CST 2020, time=2020-04-10, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1657176, encodeId=c689165e1767d, content=<a href='/topic/show?id=3ca31e71903' target=_blank style='color:#2F92EE;'>#TRA#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=26, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=17719, encryptionId=3ca31e71903, topicName=TRA)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=757b24868428, createdName=bsmagic9140, createdTime=Wed Dec 11 21:47:00 CST 2019, time=2019-12-11, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=2069253, encodeId=44c22069253fb, content=<a href='/topic/show?id=7a7135902d' target=_blank style='color:#2F92EE;'>#BMSC#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=38, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=3590, encryptionId=7a7135902d, topicName=BMSC)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=909c71, createdName=shijzhiewnjhch, createdTime=Sat May 25 13:47:00 CST 2019, time=2019-05-25, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1352144, encodeId=5dba1352144c9, content=<a href='/topic/show?id=e95954093cd' target=_blank style='color:#2F92EE;'>#成骨#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=29, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=54093, encryptionId=e95954093cd, topicName=成骨)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=4c37265, createdName=zhaojie88, createdTime=Mon May 27 03:47:00 CST 2019, time=2019-05-27, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1378668, encodeId=b63c13e8668b7, content=<a href='/topic/show?id=f238358866' target=_blank style='color:#2F92EE;'>#BMS#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=31, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=3588, encryptionId=f238358866, topicName=BMS)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=512d422, createdName=canlab, createdTime=Mon May 27 03:47:00 CST 2019, time=2019-05-27, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1406741, encodeId=67511406e41a4, content=<a href='/topic/show?id=099d122e1cd' target=_blank style='color:#2F92EE;'>#MSC#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=32, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=12271, encryptionId=099d122e1cd, topicName=MSC)], attachment=null, authenticateStatus=null, createdAvatar=http://thirdwx.qlogo.cn/mmopen/vi_32/DYAIOgq83epVh4QoCj2A7xBBEjh8q7ia2BsLkylnuQ15ia2MDzvr28XR0CVvBPpFXTlB1mprG7WgBb06gvn4Eia5Q/132, createdBy=55e02571190, createdName=huanbaofeng, createdTime=Mon May 27 03:47:00 CST 2019, time=2019-05-27, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1625934, encodeId=7061162593469, content=<a href='/topic/show?id=7435122e3c3' target=_blank style='color:#2F92EE;'>#MSCs#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=0, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=12273, encryptionId=7435122e3c3, topicName=MSCs)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=18ac20929511, createdName=zhanfl, createdTime=Mon May 27 03:47:00 CST 2019, time=2019-05-27, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1046826, encodeId=3b401046826ec, content=疗效只是效果的众多方面之一，还要看对患者的获益，包括生活质量等因素共同决定效果的, beContent=null, objectType=article, channel=null, level=null, likeNumber=21, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=f0620, createdName=CHANGE, createdTime=Sat May 25 15:47:00 CST 2019, time=2019-05-25, status=1, ipAttribution=)]
    2020-04-10 clmlylxy

    #Life#

    0

  2. [GetPortalCommentsPageByObjectIdResponse(id=2068593, encodeId=7a36206859376, content=<a href='/topic/show?id=59371088215' target=_blank style='color:#2F92EE;'>#Life#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=27, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=10882, encryptionId=59371088215, topicName=Life)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=fc8528, createdName=clmlylxy, createdTime=Fri Apr 10 16:47:00 CST 2020, time=2020-04-10, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1657176, encodeId=c689165e1767d, content=<a href='/topic/show?id=3ca31e71903' target=_blank style='color:#2F92EE;'>#TRA#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=26, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=17719, encryptionId=3ca31e71903, topicName=TRA)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=757b24868428, createdName=bsmagic9140, createdTime=Wed Dec 11 21:47:00 CST 2019, time=2019-12-11, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=2069253, encodeId=44c22069253fb, content=<a href='/topic/show?id=7a7135902d' target=_blank style='color:#2F92EE;'>#BMSC#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=38, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=3590, encryptionId=7a7135902d, topicName=BMSC)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=909c71, createdName=shijzhiewnjhch, createdTime=Sat May 25 13:47:00 CST 2019, time=2019-05-25, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1352144, encodeId=5dba1352144c9, content=<a href='/topic/show?id=e95954093cd' target=_blank style='color:#2F92EE;'>#成骨#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=29, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=54093, encryptionId=e95954093cd, topicName=成骨)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=4c37265, createdName=zhaojie88, createdTime=Mon May 27 03:47:00 CST 2019, time=2019-05-27, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1378668, encodeId=b63c13e8668b7, content=<a href='/topic/show?id=f238358866' target=_blank style='color:#2F92EE;'>#BMS#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=31, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=3588, encryptionId=f238358866, topicName=BMS)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=512d422, createdName=canlab, createdTime=Mon May 27 03:47:00 CST 2019, time=2019-05-27, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1406741, encodeId=67511406e41a4, content=<a href='/topic/show?id=099d122e1cd' target=_blank style='color:#2F92EE;'>#MSC#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=32, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=12271, encryptionId=099d122e1cd, topicName=MSC)], attachment=null, authenticateStatus=null, createdAvatar=http://thirdwx.qlogo.cn/mmopen/vi_32/DYAIOgq83epVh4QoCj2A7xBBEjh8q7ia2BsLkylnuQ15ia2MDzvr28XR0CVvBPpFXTlB1mprG7WgBb06gvn4Eia5Q/132, createdBy=55e02571190, createdName=huanbaofeng, createdTime=Mon May 27 03:47:00 CST 2019, time=2019-05-27, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1625934, encodeId=7061162593469, content=<a href='/topic/show?id=7435122e3c3' target=_blank style='color:#2F92EE;'>#MSCs#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=0, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=12273, encryptionId=7435122e3c3, topicName=MSCs)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=18ac20929511, createdName=zhanfl, createdTime=Mon May 27 03:47:00 CST 2019, time=2019-05-27, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1046826, encodeId=3b401046826ec, content=疗效只是效果的众多方面之一，还要看对患者的获益，包括生活质量等因素共同决定效果的, beContent=null, objectType=article, channel=null, level=null, likeNumber=21, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=f0620, createdName=CHANGE, createdTime=Sat May 25 15:47:00 CST 2019, time=2019-05-25, status=1, ipAttribution=)]
    2019-12-11 bsmagic9140

    #TRA#

    0

  3. [GetPortalCommentsPageByObjectIdResponse(id=2068593, encodeId=7a36206859376, content=<a href='/topic/show?id=59371088215' target=_blank style='color:#2F92EE;'>#Life#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=27, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=10882, encryptionId=59371088215, topicName=Life)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=fc8528, createdName=clmlylxy, createdTime=Fri Apr 10 16:47:00 CST 2020, time=2020-04-10, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1657176, encodeId=c689165e1767d, content=<a href='/topic/show?id=3ca31e71903' target=_blank style='color:#2F92EE;'>#TRA#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=26, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=17719, encryptionId=3ca31e71903, topicName=TRA)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=757b24868428, createdName=bsmagic9140, createdTime=Wed Dec 11 21:47:00 CST 2019, time=2019-12-11, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=2069253, encodeId=44c22069253fb, content=<a href='/topic/show?id=7a7135902d' target=_blank style='color:#2F92EE;'>#BMSC#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=38, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=3590, encryptionId=7a7135902d, topicName=BMSC)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=909c71, createdName=shijzhiewnjhch, createdTime=Sat May 25 13:47:00 CST 2019, time=2019-05-25, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1352144, encodeId=5dba1352144c9, content=<a href='/topic/show?id=e95954093cd' target=_blank style='color:#2F92EE;'>#成骨#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=29, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=54093, encryptionId=e95954093cd, topicName=成骨)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=4c37265, createdName=zhaojie88, createdTime=Mon May 27 03:47:00 CST 2019, time=2019-05-27, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1378668, encodeId=b63c13e8668b7, content=<a href='/topic/show?id=f238358866' target=_blank style='color:#2F92EE;'>#BMS#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=31, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=3588, encryptionId=f238358866, topicName=BMS)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=512d422, createdName=canlab, createdTime=Mon May 27 03:47:00 CST 2019, time=2019-05-27, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1406741, encodeId=67511406e41a4, content=<a href='/topic/show?id=099d122e1cd' target=_blank style='color:#2F92EE;'>#MSC#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=32, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=12271, encryptionId=099d122e1cd, topicName=MSC)], attachment=null, authenticateStatus=null, createdAvatar=http://thirdwx.qlogo.cn/mmopen/vi_32/DYAIOgq83epVh4QoCj2A7xBBEjh8q7ia2BsLkylnuQ15ia2MDzvr28XR0CVvBPpFXTlB1mprG7WgBb06gvn4Eia5Q/132, createdBy=55e02571190, createdName=huanbaofeng, createdTime=Mon May 27 03:47:00 CST 2019, time=2019-05-27, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1625934, encodeId=7061162593469, content=<a href='/topic/show?id=7435122e3c3' target=_blank style='color:#2F92EE;'>#MSCs#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=0, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=12273, encryptionId=7435122e3c3, topicName=MSCs)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=18ac20929511, createdName=zhanfl, createdTime=Mon May 27 03:47:00 CST 2019, time=2019-05-27, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1046826, encodeId=3b401046826ec, content=疗效只是效果的众多方面之一，还要看对患者的获益，包括生活质量等因素共同决定效果的, beContent=null, objectType=article, channel=null, level=null, likeNumber=21, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=f0620, createdName=CHANGE, createdTime=Sat May 25 15:47:00 CST 2019, time=2019-05-25, status=1, ipAttribution=)]
    2019-05-25 shijzhiewnjhch

    #BMSC#

    0

  4. [GetPortalCommentsPageByObjectIdResponse(id=2068593, encodeId=7a36206859376, content=<a href='/topic/show?id=59371088215' target=_blank style='color:#2F92EE;'>#Life#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=27, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=10882, encryptionId=59371088215, topicName=Life)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=fc8528, createdName=clmlylxy, createdTime=Fri Apr 10 16:47:00 CST 2020, time=2020-04-10, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1657176, encodeId=c689165e1767d, content=<a href='/topic/show?id=3ca31e71903' target=_blank style='color:#2F92EE;'>#TRA#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=26, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=17719, encryptionId=3ca31e71903, topicName=TRA)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=757b24868428, createdName=bsmagic9140, createdTime=Wed Dec 11 21:47:00 CST 2019, time=2019-12-11, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=2069253, encodeId=44c22069253fb, content=<a href='/topic/show?id=7a7135902d' target=_blank style='color:#2F92EE;'>#BMSC#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=38, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=3590, encryptionId=7a7135902d, topicName=BMSC)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=909c71, createdName=shijzhiewnjhch, createdTime=Sat May 25 13:47:00 CST 2019, time=2019-05-25, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1352144, encodeId=5dba1352144c9, content=<a href='/topic/show?id=e95954093cd' target=_blank style='color:#2F92EE;'>#成骨#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=29, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=54093, encryptionId=e95954093cd, topicName=成骨)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=4c37265, createdName=zhaojie88, createdTime=Mon May 27 03:47:00 CST 2019, time=2019-05-27, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1378668, encodeId=b63c13e8668b7, content=<a href='/topic/show?id=f238358866' target=_blank style='color:#2F92EE;'>#BMS#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=31, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=3588, encryptionId=f238358866, topicName=BMS)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=512d422, createdName=canlab, createdTime=Mon May 27 03:47:00 CST 2019, time=2019-05-27, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1406741, encodeId=67511406e41a4, content=<a href='/topic/show?id=099d122e1cd' target=_blank style='color:#2F92EE;'>#MSC#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=32, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=12271, encryptionId=099d122e1cd, topicName=MSC)], attachment=null, authenticateStatus=null, createdAvatar=http://thirdwx.qlogo.cn/mmopen/vi_32/DYAIOgq83epVh4QoCj2A7xBBEjh8q7ia2BsLkylnuQ15ia2MDzvr28XR0CVvBPpFXTlB1mprG7WgBb06gvn4Eia5Q/132, createdBy=55e02571190, createdName=huanbaofeng, createdTime=Mon May 27 03:47:00 CST 2019, time=2019-05-27, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1625934, encodeId=7061162593469, content=<a href='/topic/show?id=7435122e3c3' target=_blank style='color:#2F92EE;'>#MSCs#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=0, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=12273, encryptionId=7435122e3c3, topicName=MSCs)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=18ac20929511, createdName=zhanfl, createdTime=Mon May 27 03:47:00 CST 2019, time=2019-05-27, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1046826, encodeId=3b401046826ec, content=疗效只是效果的众多方面之一，还要看对患者的获益，包括生活质量等因素共同决定效果的, beContent=null, objectType=article, channel=null, level=null, likeNumber=21, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=f0620, createdName=CHANGE, createdTime=Sat May 25 15:47:00 CST 2019, time=2019-05-25, status=1, ipAttribution=)]
    2019-05-27 zhaojie88

    #成骨#

    0

  5. [GetPortalCommentsPageByObjectIdResponse(id=2068593, encodeId=7a36206859376, content=<a href='/topic/show?id=59371088215' target=_blank style='color:#2F92EE;'>#Life#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=27, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=10882, encryptionId=59371088215, topicName=Life)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=fc8528, createdName=clmlylxy, createdTime=Fri Apr 10 16:47:00 CST 2020, time=2020-04-10, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1657176, encodeId=c689165e1767d, content=<a href='/topic/show?id=3ca31e71903' target=_blank style='color:#2F92EE;'>#TRA#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=26, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=17719, encryptionId=3ca31e71903, topicName=TRA)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=757b24868428, createdName=bsmagic9140, createdTime=Wed Dec 11 21:47:00 CST 2019, time=2019-12-11, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=2069253, encodeId=44c22069253fb, content=<a href='/topic/show?id=7a7135902d' target=_blank style='color:#2F92EE;'>#BMSC#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=38, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=3590, encryptionId=7a7135902d, topicName=BMSC)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=909c71, createdName=shijzhiewnjhch, createdTime=Sat May 25 13:47:00 CST 2019, time=2019-05-25, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1352144, encodeId=5dba1352144c9, content=<a href='/topic/show?id=e95954093cd' target=_blank style='color:#2F92EE;'>#成骨#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=29, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=54093, encryptionId=e95954093cd, topicName=成骨)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=4c37265, createdName=zhaojie88, createdTime=Mon May 27 03:47:00 CST 2019, time=2019-05-27, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1378668, encodeId=b63c13e8668b7, content=<a href='/topic/show?id=f238358866' target=_blank style='color:#2F92EE;'>#BMS#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=31, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=3588, encryptionId=f238358866, topicName=BMS)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=512d422, createdName=canlab, createdTime=Mon May 27 03:47:00 CST 2019, time=2019-05-27, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1406741, encodeId=67511406e41a4, content=<a href='/topic/show?id=099d122e1cd' target=_blank style='color:#2F92EE;'>#MSC#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=32, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=12271, encryptionId=099d122e1cd, topicName=MSC)], attachment=null, authenticateStatus=null, createdAvatar=http://thirdwx.qlogo.cn/mmopen/vi_32/DYAIOgq83epVh4QoCj2A7xBBEjh8q7ia2BsLkylnuQ15ia2MDzvr28XR0CVvBPpFXTlB1mprG7WgBb06gvn4Eia5Q/132, createdBy=55e02571190, createdName=huanbaofeng, createdTime=Mon May 27 03:47:00 CST 2019, time=2019-05-27, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1625934, encodeId=7061162593469, content=<a href='/topic/show?id=7435122e3c3' target=_blank style='color:#2F92EE;'>#MSCs#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=0, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=12273, encryptionId=7435122e3c3, topicName=MSCs)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=18ac20929511, createdName=zhanfl, createdTime=Mon May 27 03:47:00 CST 2019, time=2019-05-27, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1046826, encodeId=3b401046826ec, content=疗效只是效果的众多方面之一，还要看对患者的获益，包括生活质量等因素共同决定效果的, beContent=null, objectType=article, channel=null, level=null, likeNumber=21, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=f0620, createdName=CHANGE, createdTime=Sat May 25 15:47:00 CST 2019, time=2019-05-25, status=1, ipAttribution=)]
    2019-05-27 canlab

    #BMS#

    0

  6. [GetPortalCommentsPageByObjectIdResponse(id=2068593, encodeId=7a36206859376, content=<a href='/topic/show?id=59371088215' target=_blank style='color:#2F92EE;'>#Life#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=27, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=10882, encryptionId=59371088215, topicName=Life)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=fc8528, createdName=clmlylxy, createdTime=Fri Apr 10 16:47:00 CST 2020, time=2020-04-10, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1657176, encodeId=c689165e1767d, content=<a href='/topic/show?id=3ca31e71903' target=_blank style='color:#2F92EE;'>#TRA#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=26, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=17719, encryptionId=3ca31e71903, topicName=TRA)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=757b24868428, createdName=bsmagic9140, createdTime=Wed Dec 11 21:47:00 CST 2019, time=2019-12-11, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=2069253, encodeId=44c22069253fb, content=<a href='/topic/show?id=7a7135902d' target=_blank style='color:#2F92EE;'>#BMSC#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=38, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=3590, encryptionId=7a7135902d, topicName=BMSC)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=909c71, createdName=shijzhiewnjhch, createdTime=Sat May 25 13:47:00 CST 2019, time=2019-05-25, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1352144, encodeId=5dba1352144c9, content=<a href='/topic/show?id=e95954093cd' target=_blank style='color:#2F92EE;'>#成骨#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=29, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=54093, encryptionId=e95954093cd, topicName=成骨)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=4c37265, createdName=zhaojie88, createdTime=Mon May 27 03:47:00 CST 2019, time=2019-05-27, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1378668, encodeId=b63c13e8668b7, content=<a href='/topic/show?id=f238358866' target=_blank style='color:#2F92EE;'>#BMS#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=31, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=3588, encryptionId=f238358866, topicName=BMS)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=512d422, createdName=canlab, createdTime=Mon May 27 03:47:00 CST 2019, time=2019-05-27, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1406741, encodeId=67511406e41a4, content=<a href='/topic/show?id=099d122e1cd' target=_blank style='color:#2F92EE;'>#MSC#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=32, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=12271, encryptionId=099d122e1cd, topicName=MSC)], attachment=null, authenticateStatus=null, createdAvatar=http://thirdwx.qlogo.cn/mmopen/vi_32/DYAIOgq83epVh4QoCj2A7xBBEjh8q7ia2BsLkylnuQ15ia2MDzvr28XR0CVvBPpFXTlB1mprG7WgBb06gvn4Eia5Q/132, createdBy=55e02571190, createdName=huanbaofeng, createdTime=Mon May 27 03:47:00 CST 2019, time=2019-05-27, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1625934, encodeId=7061162593469, content=<a href='/topic/show?id=7435122e3c3' target=_blank style='color:#2F92EE;'>#MSCs#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=0, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=12273, encryptionId=7435122e3c3, topicName=MSCs)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=18ac20929511, createdName=zhanfl, createdTime=Mon May 27 03:47:00 CST 2019, time=2019-05-27, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1046826, encodeId=3b401046826ec, content=疗效只是效果的众多方面之一，还要看对患者的获益，包括生活质量等因素共同决定效果的, beContent=null, objectType=article, channel=null, level=null, likeNumber=21, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=f0620, createdName=CHANGE, createdTime=Sat May 25 15:47:00 CST 2019, time=2019-05-25, status=1, ipAttribution=)]
    2019-05-27 huanbaofeng

    #MSC#

    0

  7. [GetPortalCommentsPageByObjectIdResponse(id=2068593, encodeId=7a36206859376, content=<a href='/topic/show?id=59371088215' target=_blank style='color:#2F92EE;'>#Life#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=27, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=10882, encryptionId=59371088215, topicName=Life)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=fc8528, createdName=clmlylxy, createdTime=Fri Apr 10 16:47:00 CST 2020, time=2020-04-10, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1657176, encodeId=c689165e1767d, content=<a href='/topic/show?id=3ca31e71903' target=_blank style='color:#2F92EE;'>#TRA#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=26, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=17719, encryptionId=3ca31e71903, topicName=TRA)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=757b24868428, createdName=bsmagic9140, createdTime=Wed Dec 11 21:47:00 CST 2019, time=2019-12-11, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=2069253, encodeId=44c22069253fb, content=<a href='/topic/show?id=7a7135902d' target=_blank style='color:#2F92EE;'>#BMSC#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=38, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=3590, encryptionId=7a7135902d, topicName=BMSC)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=909c71, createdName=shijzhiewnjhch, createdTime=Sat May 25 13:47:00 CST 2019, time=2019-05-25, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1352144, encodeId=5dba1352144c9, content=<a href='/topic/show?id=e95954093cd' target=_blank style='color:#2F92EE;'>#成骨#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=29, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=54093, encryptionId=e95954093cd, topicName=成骨)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=4c37265, createdName=zhaojie88, createdTime=Mon May 27 03:47:00 CST 2019, time=2019-05-27, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1378668, encodeId=b63c13e8668b7, content=<a href='/topic/show?id=f238358866' target=_blank style='color:#2F92EE;'>#BMS#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=31, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=3588, encryptionId=f238358866, topicName=BMS)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=512d422, createdName=canlab, createdTime=Mon May 27 03:47:00 CST 2019, time=2019-05-27, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1406741, encodeId=67511406e41a4, content=<a href='/topic/show?id=099d122e1cd' target=_blank style='color:#2F92EE;'>#MSC#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=32, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=12271, encryptionId=099d122e1cd, topicName=MSC)], attachment=null, authenticateStatus=null, createdAvatar=http://thirdwx.qlogo.cn/mmopen/vi_32/DYAIOgq83epVh4QoCj2A7xBBEjh8q7ia2BsLkylnuQ15ia2MDzvr28XR0CVvBPpFXTlB1mprG7WgBb06gvn4Eia5Q/132, createdBy=55e02571190, createdName=huanbaofeng, createdTime=Mon May 27 03:47:00 CST 2019, time=2019-05-27, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1625934, encodeId=7061162593469, content=<a href='/topic/show?id=7435122e3c3' target=_blank style='color:#2F92EE;'>#MSCs#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=0, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=12273, encryptionId=7435122e3c3, topicName=MSCs)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=18ac20929511, createdName=zhanfl, createdTime=Mon May 27 03:47:00 CST 2019, time=2019-05-27, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1046826, encodeId=3b401046826ec, content=疗效只是效果的众多方面之一，还要看对患者的获益，包括生活质量等因素共同决定效果的, beContent=null, objectType=article, channel=null, level=null, likeNumber=21, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=f0620, createdName=CHANGE, createdTime=Sat May 25 15:47:00 CST 2019, time=2019-05-25, status=1, ipAttribution=)]
    2019-05-27 zhanfl

    #MSCs#

    0

  8. [GetPortalCommentsPageByObjectIdResponse(id=2068593, encodeId=7a36206859376, content=<a href='/topic/show?id=59371088215' target=_blank style='color:#2F92EE;'>#Life#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=27, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=10882, encryptionId=59371088215, topicName=Life)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=fc8528, createdName=clmlylxy, createdTime=Fri Apr 10 16:47:00 CST 2020, time=2020-04-10, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1657176, encodeId=c689165e1767d, content=<a href='/topic/show?id=3ca31e71903' target=_blank style='color:#2F92EE;'>#TRA#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=26, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=17719, encryptionId=3ca31e71903, topicName=TRA)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=757b24868428, createdName=bsmagic9140, createdTime=Wed Dec 11 21:47:00 CST 2019, time=2019-12-11, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=2069253, encodeId=44c22069253fb, content=<a href='/topic/show?id=7a7135902d' target=_blank style='color:#2F92EE;'>#BMSC#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=38, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=3590, encryptionId=7a7135902d, topicName=BMSC)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=909c71, createdName=shijzhiewnjhch, createdTime=Sat May 25 13:47:00 CST 2019, time=2019-05-25, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1352144, encodeId=5dba1352144c9, content=<a href='/topic/show?id=e95954093cd' target=_blank style='color:#2F92EE;'>#成骨#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=29, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=54093, encryptionId=e95954093cd, topicName=成骨)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=4c37265, createdName=zhaojie88, createdTime=Mon May 27 03:47:00 CST 2019, time=2019-05-27, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1378668, encodeId=b63c13e8668b7, content=<a href='/topic/show?id=f238358866' target=_blank style='color:#2F92EE;'>#BMS#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=31, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=3588, encryptionId=f238358866, topicName=BMS)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=512d422, createdName=canlab, createdTime=Mon May 27 03:47:00 CST 2019, time=2019-05-27, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1406741, encodeId=67511406e41a4, content=<a href='/topic/show?id=099d122e1cd' target=_blank style='color:#2F92EE;'>#MSC#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=32, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=12271, encryptionId=099d122e1cd, topicName=MSC)], attachment=null, authenticateStatus=null, createdAvatar=http://thirdwx.qlogo.cn/mmopen/vi_32/DYAIOgq83epVh4QoCj2A7xBBEjh8q7ia2BsLkylnuQ15ia2MDzvr28XR0CVvBPpFXTlB1mprG7WgBb06gvn4Eia5Q/132, createdBy=55e02571190, createdName=huanbaofeng, createdTime=Mon May 27 03:47:00 CST 2019, time=2019-05-27, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1625934, encodeId=7061162593469, content=<a href='/topic/show?id=7435122e3c3' target=_blank style='color:#2F92EE;'>#MSCs#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=0, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=12273, encryptionId=7435122e3c3, topicName=MSCs)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=18ac20929511, createdName=zhanfl, createdTime=Mon May 27 03:47:00 CST 2019, time=2019-05-27, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1046826, encodeId=3b401046826ec, content=疗效只是效果的众多方面之一，还要看对患者的获益，包括生活质量等因素共同决定效果的, beContent=null, objectType=article, channel=null, level=null, likeNumber=21, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=f0620, createdName=CHANGE, createdTime=Sat May 25 15:47:00 CST 2019, time=2019-05-25, status=1, ipAttribution=)]
    2019-05-25 CHANGE

    疗效只是效果的众多方面之一，还要看对患者的获益，包括生活质量等因素共同决定效果的

    0