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J Cell Physiol：化的脂肪来源的干细胞共培养物用于RADA16-I中的骨再生

2018-08-07 MedSci MedSci原创

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已知脂肪来源的干细胞（ADSCs）具有成骨和血管形成的巨大潜力，因此，探索分化的ADSCs的共培养物以增加骨和血管形成。在这项研究中，ADSCs被诱导成骨原性ADSCs（os-ADSCs）和内皮ADSCs（endo-ADSC）细胞，然后以不同比例共培养（os-ADSCs/endo-ADSCs = 2：1,1：1,1 ：2）。os-ADSC以1：1的比例可表达更高水平的ALP，RUNX2和COL-1

已知脂肪来源的干细胞（ADSCs）具有成骨和血管形成的巨大潜力，因此，探索分化的ADSCs的共培养物以增加骨和血管形成。

在这项研究中，ADSCs被诱导成骨原性ADSCs（os-ADSCs）和内皮ADSCs（endo-ADSC）细胞，然后以不同比例共培养（os-ADSCs/endo-ADSCs = 2：1,1：1,1 ：2）。os-ADSC以1：1的比例可表达更高水平的ALP，RUNX2和COL-1，而VEGF，vWF和CD31在该组的endo-ADSC中上调。

行下一代RNA测序（RNA-seq）以评估共培养的ADSC的分子机制。结果显示，os-ADSCs和endo-ADSCs在成骨和血管生成分化过程中相互作用，尤其是比例为1：1，并受血管相关基因，细胞介导基因，骨相关基因和转化生长因子β信号通路（TGF-β），丝裂原活化蛋白激酶信号通路（MAPK）和wnt信号通路（Wnt）的调节。研究还检测到Angptl4，apoe，mmp3，bmp6，mmp13和fgf18的表达上调，而cxcl12和wnt5a的表达下调。结果表明，基因表达水平与RNA-seq中的基因表达水平一致。

然后将细胞接种到自组装肽RADA16-I支架中作为共培养物（1：1）和单一培养物（ADSCs，os-ADSCs，endo-ADSCs）。结果表明，各组细胞均在支架上生长和增殖良好，共培养组具有较好的成骨和血管形成。

总之，该研究结果表明，共培养的os-ADSC和endo-ADSC以1：1的可显著增强成骨和血管生成分化。以1：1的比例在自组装肽RADA16-I支架中通过3D培养细胞具有较大的成骨和血管形成潜力，仍需进一步的体内骨再生评估。

原始出处：

Yang H, Hong N, et al., Differentiated adipose-derived stem cell cocultures for bone regeneration in RADA16-I in vitro. J Cell Physiol. 2018 Jul 11. doi: 10.1002/jcp.26838.

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    2018-08-29 zhaozhouchifen

    #Cell#

    0

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    2018-10-08 维他命

    #CEL#

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    2019-07-12 cmj8wellington

    #Physio#

    0

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beContent=null, objectType=article, channel=null, level=null, likeNumber=43, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=http://cdnapi.center.medsci.cn/medsci/head/2019/12/23/2f51002e1349ccda5be11c1eea1e1e60.jpg, createdBy=a22097948, createdName=天地飞扬, createdTime=Tue Aug 07 23:18:34 CST 2018, time=2018-08-07, status=1, ipAttribution=)]
    2018-09-01 neizongke

    #细胞共培养#

    0

  5. 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beContent=null, objectType=article, channel=null, level=null, likeNumber=43, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=http://cdnapi.center.medsci.cn/medsci/head/2019/12/23/2f51002e1349ccda5be11c1eea1e1e60.jpg, createdBy=a22097948, createdName=天地飞扬, createdTime=Tue Aug 07 23:18:34 CST 2018, time=2018-08-07, status=1, ipAttribution=)]
    2018-08-23 zwjnj2

    好好学习天天向上

    0

  6. 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beContent=null, objectType=article, channel=null, level=null, likeNumber=43, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=http://cdnapi.center.medsci.cn/medsci/head/2019/12/23/2f51002e1349ccda5be11c1eea1e1e60.jpg, createdBy=a22097948, createdName=天地飞扬, createdTime=Tue Aug 07 23:18:34 CST 2018, time=2018-08-07, status=1, ipAttribution=)]
    2018-08-21 zwjnj2

    好好学习天天向上

    0

  7. 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href='/topic/show?id=d068101610b9' target=_blank style='color:#2F92EE;'>#骨再生#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=16, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=101610, encryptionId=d068101610b9, topicName=骨再生)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=a4cf7208806, createdName=lsj630, createdTime=Thu Aug 09 13:02:00 CST 2018, time=2018-08-09, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=337187, encodeId=dd8633e1870e, content=学习了，谢谢分享, beContent=null, objectType=article, channel=null, level=null, likeNumber=51, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=7c592194322, createdName=1209e435m98(暂无昵称), createdTime=Tue Aug 07 23:20:12 CST 2018, time=2018-08-07, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=337182, encodeId=b7ce33e18204, content=了解一下，谢谢分享！, beContent=null, objectType=article, channel=null, level=null, likeNumber=43, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=http://cdnapi.center.medsci.cn/medsci/head/2019/12/23/2f51002e1349ccda5be11c1eea1e1e60.jpg, createdBy=a22097948, createdName=天地飞扬, createdTime=Tue Aug 07 23:18:34 CST 2018, time=2018-08-07, status=1, ipAttribution=)]
    2018-08-09 般若傻瓜

    #ADA#

    0

  8. [GetPortalCommentsPageByObjectIdResponse(id=1870801, encodeId=e64e18e080179, content=<a href='/topic/show?id=fd764459a8' target=_blank style='color:#2F92EE;'>#Cell#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=18, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=4459, encryptionId=fd764459a8, topicName=Cell)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=524d95, createdName=zhaozhouchifen, createdTime=Wed Aug 29 17:02:00 CST 2018, time=2018-08-29, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1961071, encodeId=c0c119610e1f6, content=<a href='/topic/show?id=dbe6445416' target=_blank style='color:#2F92EE;'>#CEL#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=28, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=4454, encryptionId=dbe6445416, topicName=CEL)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=c3ff68, createdName=维他命, createdTime=Mon Oct 08 03:02:00 CST 2018, time=2018-10-08, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1788861, encodeId=dda21e888615f, content=<a href='/topic/show?id=866d1421e4c' target=_blank style='color:#2F92EE;'>#Physio#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=30, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=14217, encryptionId=866d1421e4c, topicName=Physio)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=c689267, createdName=cmj8wellington, createdTime=Fri Jul 12 04:02:00 CST 2019, time=2019-07-12, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1804946, encodeId=aca21804946ee, content=<a href='/topic/show?id=ac3fe779792' target=_blank style='color:#2F92EE;'>#细胞共培养#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=26, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=77797, 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topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/Q3auHgzwzM6DGOvNrRdcib3UmzMgpEQpl0ENSJScphsFtdgfibXX1g1IuEYGCjP23UqCd52TxTpSZibtPiclyusaHicnNXd78alaia19Qy1nAFo18/0, createdBy=50381491660, createdName=zwjnj2, createdTime=Tue Aug 21 15:16:34 CST 2018, time=2018-08-21, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1264664, encodeId=3f7b1264664e9, content=<a href='/topic/show?id=150f19229c' target=_blank style='color:#2F92EE;'>#ADA#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=29, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=1922, encryptionId=150f19229c, topicName=ADA)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=ad9f79, createdName=般若傻瓜, createdTime=Thu Aug 09 13:02:00 CST 2018, time=2018-08-09, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1472144, encodeId=8e5814e2144c0, content=<a href='/topic/show?id=d068101610b9' target=_blank style='color:#2F92EE;'>#骨再生#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=16, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=101610, encryptionId=d068101610b9, topicName=骨再生)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=a4cf7208806, createdName=lsj630, createdTime=Thu Aug 09 13:02:00 CST 2018, time=2018-08-09, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=337187, encodeId=dd8633e1870e, content=学习了，谢谢分享, beContent=null, objectType=article, channel=null, level=null, likeNumber=51, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=7c592194322, createdName=1209e435m98(暂无昵称), createdTime=Tue Aug 07 23:20:12 CST 2018, time=2018-08-07, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=337182, encodeId=b7ce33e18204, content=了解一下，谢谢分享！, beContent=null, objectType=article, channel=null, level=null, likeNumber=43, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=http://cdnapi.center.medsci.cn/medsci/head/2019/12/23/2f51002e1349ccda5be11c1eea1e1e60.jpg, createdBy=a22097948, createdName=天地飞扬, createdTime=Tue Aug 07 23:18:34 CST 2018, time=2018-08-07, status=1, ipAttribution=)]
    2018-08-09 lsj630

    #骨再生#

    0

  9. [GetPortalCommentsPageByObjectIdResponse(id=1870801, encodeId=e64e18e080179, content=<a href='/topic/show?id=fd764459a8' target=_blank style='color:#2F92EE;'>#Cell#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=18, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=4459, encryptionId=fd764459a8, topicName=Cell)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=524d95, createdName=zhaozhouchifen, createdTime=Wed Aug 29 17:02:00 CST 2018, time=2018-08-29, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1961071, encodeId=c0c119610e1f6, content=<a href='/topic/show?id=dbe6445416' target=_blank style='color:#2F92EE;'>#CEL#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=28, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=4454, encryptionId=dbe6445416, topicName=CEL)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=c3ff68, 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beContent=null, objectType=article, channel=null, level=null, likeNumber=43, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=http://cdnapi.center.medsci.cn/medsci/head/2019/12/23/2f51002e1349ccda5be11c1eea1e1e60.jpg, createdBy=a22097948, createdName=天地飞扬, createdTime=Tue Aug 07 23:18:34 CST 2018, time=2018-08-07, status=1, ipAttribution=)]
    2018-08-07 1209e435m98(暂无昵称)

    学习了，谢谢分享

    0

  10. 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beContent=null, objectType=article, channel=null, level=null, likeNumber=43, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=http://cdnapi.center.medsci.cn/medsci/head/2019/12/23/2f51002e1349ccda5be11c1eea1e1e60.jpg, createdBy=a22097948, createdName=天地飞扬, createdTime=Tue Aug 07 23:18:34 CST 2018, time=2018-08-07, status=1, ipAttribution=)]
    2018-08-07 天地飞扬

    了解一下，谢谢分享！

    0

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