Anal Biochem:纳米材料用于乙型肝炎病毒 的检测

2018-04-05 MedSci MedSci原创

这项工作描述了使用电化学和光学技术检测乙型肝炎病毒(HBV)基因组DNA的不同方法。该检测方法由对HBV具有特异性的单链DNA探针(HEPB1S)组成,嫁接在还原型氧化石墨烯或金纳米颗粒修饰的金电极上。 差分脉冲伏安法分析表明,当与阴性对照相比时,HBV基因组DNA的添加导致当前峰值增加约1.4倍。与对数HBV-基因组DNA浓度和检测的电化学生物传感器之间存在线性相关性,直到目标为7.65p

这项工作描述了使用电化学和光学技术检测乙型肝炎病毒(HBV)基因组DNA的不同方法。该检测方法由对HBV具有特异性的单链DNA探针(HEPB1S)组成,嫁接在还原型氧化石墨烯或金纳米颗粒修饰的金电极上。

差分脉冲伏安法分析表明,当与阴性对照相比时,HBV基因组DNA的添加导致当前峰值增加约1.4倍。与对数HBV-基因组DNA浓度和检测的电化学生物传感器之间存在线性相关性,直到目标为7.65pgμL-1。加入HBV基因组DNA后,电化学阻抗谱测量显示电荷转移抗性增加约2倍。使用金纳米颗粒的胶体悬浮液的测定显示峰波长的移动,与HBV基因组DNA浓度成线性比例,检测极限为0.15ngμL-1。

为用于临床样品的检测,金纳米颗粒在血浆中测试成功。在这项工作中使用的所有方法都能有效检测HBV阳性样本中的基因组DNA或血浆。

原始出处:

Oliveira DA, Silva JV, et al., Application of nanomaterials for the electrical and optical detection of the hepatitis B virus. Anal Biochem. 2018 Mar 27;549:157-163. doi: 10.1016/j.ab.2018.03.023.

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    2018-11-01 经常头晕
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    2019-03-02 AspirantSuo
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level=null, likeNumber=0, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/mStl88fu4NfNLvzZhgPxRrYOibkVibW9vAkCfb2F55aHjqMoqUVaF9fuXIMCxexawSOsicpbF7Ria7o54zPK65PodlkwhPrNhLhS/0, createdBy=12f71314239, createdName=ann y, createdTime=Thu Apr 05 17:18:02 CST 2018, time=2018-04-05, status=1, ipAttribution=)]
    2018-10-27 sunylz
  5. 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  6. 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  7. 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attachment=null, authenticateStatus=null, createdAvatar=https://img.medsci.cn/20220115/46bcf39c32de4aa6b45c5f9d66c8ee77/6cb4a20c55bb4b7691122f47747bfca2.jpg, createdBy=9dad1662329, createdName=1ddf0692m34(暂无匿称), createdTime=Thu Apr 05 17:37:53 CST 2018, time=2018-04-05, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=303033, encodeId=7a38303033a3, content=纳米材料用于乙型肝炎病毒 的检测, beContent=null, objectType=article, channel=null, level=null, likeNumber=66, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/mStl88fu4NfNLvzZhgPxRrYOibkVibW9vAkCfb2F55aHjqMoqUVaF9fuXIMCxexawSOsicpbF7Ria7o54zPK65PodlkwhPrNhLhS/0, createdBy=12f71314239, createdName=ann y, createdTime=Thu Apr 05 17:18:15 CST 2018, time=2018-04-05, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=303032, encodeId=705430303259, content=纳米材料用于乙型肝炎病毒 的检测, beContent=null, objectType=article, channel=null, level=null, likeNumber=0, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/mStl88fu4NfNLvzZhgPxRrYOibkVibW9vAkCfb2F55aHjqMoqUVaF9fuXIMCxexawSOsicpbF7Ria7o54zPK65PodlkwhPrNhLhS/0, createdBy=12f71314239, createdName=ann y, createdTime=Thu Apr 05 17:18:02 CST 2018, time=2018-04-05, status=1, ipAttribution=)]
    2018-04-06 张新亮1853311252142e2fm

    good好好.好文献

    0

  8. 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level=null, likeNumber=0, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/mStl88fu4NfNLvzZhgPxRrYOibkVibW9vAkCfb2F55aHjqMoqUVaF9fuXIMCxexawSOsicpbF7Ria7o54zPK65PodlkwhPrNhLhS/0, createdBy=12f71314239, createdName=ann y, createdTime=Thu Apr 05 17:18:02 CST 2018, time=2018-04-05, status=1, ipAttribution=)]
    2018-04-05 1ddf0692m34(暂无匿称)

    学习了.谢谢分享

    0

  9. 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level=null, likeNumber=0, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/mStl88fu4NfNLvzZhgPxRrYOibkVibW9vAkCfb2F55aHjqMoqUVaF9fuXIMCxexawSOsicpbF7Ria7o54zPK65PodlkwhPrNhLhS/0, createdBy=12f71314239, createdName=ann y, createdTime=Thu Apr 05 17:18:02 CST 2018, time=2018-04-05, status=1, ipAttribution=)]
    2018-04-05 ann y

    纳米材料用于乙型肝炎病毒 的检测

    0

  10. 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level=null, likeNumber=0, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/mStl88fu4NfNLvzZhgPxRrYOibkVibW9vAkCfb2F55aHjqMoqUVaF9fuXIMCxexawSOsicpbF7Ria7o54zPK65PodlkwhPrNhLhS/0, createdBy=12f71314239, createdName=ann y, createdTime=Thu Apr 05 17:18:02 CST 2018, time=2018-04-05, status=1, ipAttribution=)]
    2018-04-05 ann y

    纳米材料用于乙型肝炎病毒 的检测

    0

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