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PLoS One：S53P4基硼硅酸盐玻璃和玻璃溶出产物对人脂肪干细胞成骨作用的影响

2018-09-02 MedSci MedSci原创

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尽管硅酸盐生物活性玻璃在骨再生中具有良好的性能，但是通过化学改性提高其性能仍具有相当大的潜力。在该研究中，研究人员合成了基于S53P4的硼硅酸盐玻璃，并通过评估pH变化，离子释放和向羟基磷灰石的转化来研究其在模拟体液中的溶出曲线。在玻璃盘和提取培养基中直接培养评估人脂肪干细胞（hASCs）的活力，增殖，附着，成骨和内皮标记物表达。这是第一项评估基于S53P4（商品名为BonAlive®）的硼硅酸盐

尽管硅酸盐生物活性玻璃在骨再生中具有良好的性能，但是通过化学改性提高其性能仍具有相当大的潜力。在该研究中，研究人员合成了基于S53P4的硼硅酸盐玻璃，并通过评估pH变化，离子释放和向羟基磷灰石的转化来研究其在模拟体液中的溶出曲线。

在玻璃盘和提取培养基中直接培养评估人脂肪干细胞（hASCs）的活力，增殖，附着，成骨和内皮标记物表达。这是第一项评估基于S53P4（商品名为BonAlive®）的硼硅酸盐玻璃的细胞行为的研究。在S53P4中用硼酸盐代替硅酸盐增加了玻璃反应性。尽管在所有条件下hASCs都具有良好的活力，但是在硼硅酸盐圆盘和未稀释的提取物培养基中直接培养细胞减少了细胞增殖，同时伴随着细胞形态的变化。

成骨方面，硼硅酸盐玻璃盘和提取物显著降低了碱性磷酸酶活性，而成骨标记物RUNX2a，OSTERIX，DLX5和OSTEOPONTIN的表达上调。硼硅酸盐玻璃还增加了骨钙蛋白的产生。此外，与成骨培养基对照相比，含有硼硅酸盐提取物的成骨补充剂显著增加了矿物质产量。有趣的是，硼硅酸盐玻璃刺激了内皮标志物vWF和PECAM-1的表达。

总之，我们的研究结果表明，尽管减少hASCs的增殖，基于S53P4的硼硅酸盐玻璃及其溶出产物刺激成骨并上调内皮标记物，从而支持其对再生医学的进一步评估。

原始出处：

Ojansivu M, Mishra A, et al., The effect of S53P4-based borosilicate glasses and glass dissolution products on the osteogenic commitment of human adipose stem cells. PLoS One. 2018 Aug 28;13(8):e0202740. doi: 10.1371/journal.pone.0202740. eCollection 2018.

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    2018-12-28 119337457

    #Plos one#

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    #成骨#

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