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J Dent Res：Amelx-iCre转基因小鼠中Stim1对成釉细胞特异性的作用

2019-07-24 lishiting MedSci原创

Cre工具鼠釉原蛋白转基因
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在口腔研究中，对调节釉质发生的分子通路的鉴定和检验一直都是该领域的难点，并且研究进展受限于对成釉细胞基因功能有限的基因研究方法。这篇研究中，作者构建了4种Cre驱动的转基因小鼠系，它们是将小鼠成釉细胞特异性基因釉原蛋白X (Amelx-iCre)位点插入细菌人工染色体DNA载体(250-kb)构建而成，表达改良后的Cre (iCre)-重组酶。

在口腔研究中，对调节釉质发生的分子通路的鉴定和检验一直都是该领域的难点，并且研究进展受限于对成釉细胞基因功能有限的基因研究方法。这篇研究中，作者构建了4种Cre驱动的转基因小鼠系，它们是将小鼠成釉细胞特异性基因釉原蛋白X (Amelx-iCre)位点插入细菌人工染色体DNA载体(250-kb)构建而成，表达改良后的Cre (iCre)-重组酶。

所有的4 个Amelx-iCre鼠系与ROSA26报告基因小鼠交配，通过LacZ基因编码b-半乳糖苷酶活性检测和Cre蛋白免疫组化鉴定iCre的发育模式。4个转基因鼠系中2个在成釉细胞中高表达Cre重组酶并且在发育阶段以及细胞特异性的展现出模拟内源性釉原蛋白表达的b-半乳糖苷酶活性。为了检测所选2个转基因模型的功能，研究将2个Amelx-iCre鼠系与stromal interaction molecule 1 (Stim1) floxed小鼠交配以产生成釉细胞特异性条件敲除Stim1的转基因小鼠(Stim1 cKO)模型。

STIM1是成釉细胞中一个主要的钙感受器，在釉质矿化和成釉细胞分化过程中起到重要作用。Amelx-iCre小鼠在切牙和磨牙成釉细胞中展现出独特的CRE介导的重组。Stim1 cKO鼠展现出釉质表型的严重缺陷，包括降低的结构完整性伴随磨耗增加以及牙齿变小。Amelx-iCre/Stim1 cKO的表型和基因型与之前报道的Ker14-Cre/Stim1 cKO存在显著差异，需要在细胞和发育阶段Cre鼠系进行表型和基因型的准确比较。另外，此模型携带的是整个釉原蛋白基因位点，而不是仅限于釉原蛋白的部分启动子结构。因此，此种结构极大的增强了Cre表达的稳定性和特异性。

此种Amelx-iCre转基因鼠系在将来的调查研究中可以作为一种靶向成釉细胞特异性基因表达的强有力工具。

原始出处：

Said R， Zheng L， et al. Generation of Amelx-iCre Mice Supports Ameloblast-Specific Role for Stim1. J Dent Res. 2019 Aug；98(9)：1002-1010. doi： 10.1177/0022034519858976.

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    2020-06-15 ms920641618706883

    #成釉细胞#

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    2020-04-18 wgsun

    #细胞特异性#

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    2019-10-23 jinweidong

    #特异性#

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    2019-07-26 旅苦化文_210

    #转基因#

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    2019-07-26 41514490@qq.com

    #转基因小鼠#

    0

  6. [GetPortalCommentsPageByObjectIdResponse(id=1956906, encodeId=b5321956906e3, content=<a href='/topic/show?id=8788540865a' target=_blank style='color:#2F92EE;'>#成釉细胞#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=25, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=54086, encryptionId=8788540865a, topicName=成釉细胞)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=aa5a2500103, createdName=ms920641618706883, createdTime=Mon Jun 15 22:56:00 CST 2020, time=2020-06-15, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1993746, encodeId=df3b1993e4679, content=<a href='/topic/show?id=f7c4e79590d' target=_blank style='color:#2F92EE;'>#细胞特异性#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=35, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=77959, encryptionId=f7c4e79590d, topicName=细胞特异性)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=346311, createdName=wgsun, createdTime=Sat Apr 18 02:56:00 CST 2020, time=2020-04-18, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1899313, encodeId=15fd189931315, content=<a href='/topic/show?id=6f746e94170' target=_blank style='color:#2F92EE;'>#特异性#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=27, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=67941, encryptionId=6f746e94170, topicName=特异性)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=dc7a157, createdName=jinweidong, createdTime=Wed Oct 23 15:56:00 CST 2019, time=2019-10-23, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1471925, encodeId=084414e1925f3, content=<a href='/topic/show?id=e97a93305ec' target=_blank style='color:#2F92EE;'>#转基因#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=39, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=93305, encryptionId=e97a93305ec, topicName=转基因)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=76227191338, createdName=旅苦化文_210, createdTime=Fri Jul 26 03:56:00 CST 2019, time=2019-07-26, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1516266, encodeId=21d2151626620, content=<a href='/topic/show?id=a5e09331605' target=_blank style='color:#2F92EE;'>#转基因小鼠#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=34, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=93316, encryptionId=a5e09331605, topicName=转基因小鼠)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=7a8010946846, createdName=41514490@qq.com, createdTime=Fri Jul 26 03:56:00 CST 2019, time=2019-07-26, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1581484, encodeId=946b1581484f0, content=<a href='/topic/show?id=2bc1519579' target=_blank style='color:#2F92EE;'>#CRE#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=23, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=5195, encryptionId=2bc1519579, topicName=CRE)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=4ca216802819, createdName=HinsMax, createdTime=Fri Jul 26 03:56:00 CST 2019, time=2019-07-26, status=1, ipAttribution=)]
    2019-07-26 HinsMax

    #CRE#

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