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Biochemia Medica：高效液相色谱-高分辨质谱联用法检测hepcidin-25的方法

2019-08-03 不详 MedSci原创

hepcidin-25
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在临床研究中，对铁代谢的关键调节因子<span lang="EN-US" style="font-size:12.0pt;mso-bidi-font-size:14.0pt;font-family:&quot;Calibri&quot;,&quot;sans-serif&quot;; mso-fareast-font-family:宋体;mso-bidi-font-family:&quot;Ti

在临床研究中，对铁代谢的关键调节因子hepcidin-25的准确测定是非常重要的。本研究旨在评价液相色谱-串联质谱(LC-MS/MS)试剂盒(immunstik AG, Bensheim, Germany)定量检测Hepcidin-25蛋白的性能。

研究人员评价了液相色谱高分辨质谱法(LC-HR-MS)的精密度、准确度、线性度和分析前要求。将不精确性和偏差接受准则确定为≤15%。研究了样品在室温(RT)和反复冻融循环后的稳定性。此外，还评估了165名健康成年人的血清hepcidin-25浓度以作为参考。

研究显示，hepcidin-25 LC-MS/MS在3 - 200ng /mL浓度范围内呈线性变化。运行内和运行间的精度分别在1.9 - 8.6%和5.1 - 12.4%之间。低高对照材料的平均偏倚分别为- 2.7%和2.1%。RT血清样本稳定3小时(平均偏倚+ 0.3%)。经过2次和3次冻融循环后，hepcidin-25浓度偏倚分别为+ 8.0和+ 20%。在165名健康成年人中，109名女性的中位数为8.42(范围:1.00 - 60.10)ng/mL，明显低于56名男性的中位数15.76(范围:1.50 - 60.50)ng/mL (P = 0.002)。

研究表明，hepcidin-25 LC-MS/MS分析范围广，并满足≤15%的不精确性和偏倚接受标准。血清样品可在RT条件下保存3小时，并能承受两次冻融循环。

原始出处：

Dietmar Enko,Sieglinde Zelzer,Evaluation of a commercial liquid-chromatography high-resolution mass-spectrometry method for the determination of hepcidin-25

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    2020-05-17 sunylz

    #Bio#

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    2019-12-20 jeanqiuqiu

    #DIC#

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    2019-12-17 lsndxfj

    #高分辨#

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    2020-01-15 tm10051993

    #Hepcidin#

    0

  5. [GetPortalCommentsPageByObjectIdResponse(id=1918797, encodeId=faa61918e9779, content=<a href='/topic/show?id=4f26341994' target=_blank style='color:#2F92EE;'>#Bio#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=22, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=3419, encryptionId=4f26341994, topicName=Bio)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=12de429, createdName=sunylz, createdTime=Sun May 17 19:53:00 CST 2020, time=2020-05-17, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1858975, encodeId=7a5418589e578, content=<a href='/topic/show?id=e0a9593596' target=_blank style='color:#2F92EE;'>#DIC#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=31, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=5935, encryptionId=e0a9593596, topicName=DIC)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=6f7770, createdName=jeanqiuqiu, createdTime=Fri Dec 20 17:53:00 CST 2019, time=2019-12-20, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1997614, encodeId=273a199e6140c, content=<a href='/topic/show?id=e06810222253' target=_blank style='color:#2F92EE;'>#高分辨#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=35, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=102222, encryptionId=e06810222253, topicName=高分辨)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=acc3210, createdName=lsndxfj, createdTime=Tue Dec 17 03:53:00 CST 2019, time=2019-12-17, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1752210, encodeId=027d1e522101a, content=<a href='/topic/show?id=f2188e0859' target=_blank style='color:#2F92EE;'>#Hepcidin#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=31, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=8708, encryptionId=f2188e0859, topicName=Hepcidin)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=f7a536471202, createdName=tm10051993, createdTime=Wed Jan 15 09:53:00 CST 2020, time=2020-01-15, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1991613, encodeId=71901991613a6, content=<a href='/topic/show?id=f88a4e047f' target=_blank style='color:#2F92EE;'>#CID#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=29, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=4704, encryptionId=f88a4e047f, topicName=CID)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=570f404, createdName=xlxchina, createdTime=Sat Apr 18 10:53:00 CST 2020, time=2020-04-18, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1553356, encodeId=a5c7155335609, content=<a href='/topic/show?id=81bd923e559' target=_blank style='color:#2F92EE;'>#质谱#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=29, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=92375, encryptionId=81bd923e559, topicName=质谱)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=96fd14366106, createdName=jeanqiuqiu, createdTime=Mon Aug 05 13:53:00 CST 2019, time=2019-08-05, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1609904, encodeId=74071609904e5, content=<a href='/topic/show?id=eb6b1145939' target=_blank style='color:#2F92EE;'>#Med#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=25, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=11459, encryptionId=eb6b1145939, topicName=Med)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=552a19396740, createdName=ms3994565386320060, createdTime=Mon Aug 05 13:53:00 CST 2019, time=2019-08-05, status=1, ipAttribution=)]
    2020-04-18 xlxchina

    #CID#

    0

  6. [GetPortalCommentsPageByObjectIdResponse(id=1918797, encodeId=faa61918e9779, content=<a href='/topic/show?id=4f26341994' target=_blank style='color:#2F92EE;'>#Bio#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=22, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=3419, encryptionId=4f26341994, topicName=Bio)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=12de429, createdName=sunylz, createdTime=Sun May 17 19:53:00 CST 2020, time=2020-05-17, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1858975, encodeId=7a5418589e578, content=<a href='/topic/show?id=e0a9593596' target=_blank style='color:#2F92EE;'>#DIC#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=31, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=5935, encryptionId=e0a9593596, topicName=DIC)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=6f7770, createdName=jeanqiuqiu, createdTime=Fri Dec 20 17:53:00 CST 2019, time=2019-12-20, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1997614, encodeId=273a199e6140c, content=<a href='/topic/show?id=e06810222253' target=_blank style='color:#2F92EE;'>#高分辨#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=35, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=102222, encryptionId=e06810222253, topicName=高分辨)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=acc3210, createdName=lsndxfj, createdTime=Tue Dec 17 03:53:00 CST 2019, time=2019-12-17, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1752210, encodeId=027d1e522101a, content=<a href='/topic/show?id=f2188e0859' target=_blank style='color:#2F92EE;'>#Hepcidin#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=31, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=8708, encryptionId=f2188e0859, topicName=Hepcidin)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=f7a536471202, createdName=tm10051993, createdTime=Wed Jan 15 09:53:00 CST 2020, time=2020-01-15, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1991613, encodeId=71901991613a6, content=<a href='/topic/show?id=f88a4e047f' target=_blank style='color:#2F92EE;'>#CID#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=29, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=4704, encryptionId=f88a4e047f, topicName=CID)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=570f404, createdName=xlxchina, createdTime=Sat Apr 18 10:53:00 CST 2020, time=2020-04-18, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1553356, encodeId=a5c7155335609, content=<a href='/topic/show?id=81bd923e559' target=_blank style='color:#2F92EE;'>#质谱#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=29, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=92375, encryptionId=81bd923e559, topicName=质谱)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=96fd14366106, createdName=jeanqiuqiu, createdTime=Mon Aug 05 13:53:00 CST 2019, time=2019-08-05, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1609904, encodeId=74071609904e5, content=<a href='/topic/show?id=eb6b1145939' target=_blank style='color:#2F92EE;'>#Med#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=25, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=11459, encryptionId=eb6b1145939, topicName=Med)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=552a19396740, createdName=ms3994565386320060, createdTime=Mon Aug 05 13:53:00 CST 2019, time=2019-08-05, status=1, ipAttribution=)]
    2019-08-05 jeanqiuqiu

    #质谱#

    0

  7. [GetPortalCommentsPageByObjectIdResponse(id=1918797, encodeId=faa61918e9779, content=<a href='/topic/show?id=4f26341994' target=_blank style='color:#2F92EE;'>#Bio#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=22, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=3419, encryptionId=4f26341994, topicName=Bio)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=12de429, createdName=sunylz, createdTime=Sun May 17 19:53:00 CST 2020, time=2020-05-17, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1858975, encodeId=7a5418589e578, content=<a href='/topic/show?id=e0a9593596' target=_blank style='color:#2F92EE;'>#DIC#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=31, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=5935, encryptionId=e0a9593596, topicName=DIC)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=6f7770, createdName=jeanqiuqiu, createdTime=Fri Dec 20 17:53:00 CST 2019, time=2019-12-20, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1997614, encodeId=273a199e6140c, content=<a href='/topic/show?id=e06810222253' target=_blank style='color:#2F92EE;'>#高分辨#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=35, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=102222, encryptionId=e06810222253, topicName=高分辨)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=acc3210, createdName=lsndxfj, createdTime=Tue Dec 17 03:53:00 CST 2019, time=2019-12-17, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1752210, encodeId=027d1e522101a, content=<a href='/topic/show?id=f2188e0859' target=_blank style='color:#2F92EE;'>#Hepcidin#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=31, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=8708, encryptionId=f2188e0859, topicName=Hepcidin)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=f7a536471202, createdName=tm10051993, createdTime=Wed Jan 15 09:53:00 CST 2020, time=2020-01-15, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1991613, encodeId=71901991613a6, content=<a href='/topic/show?id=f88a4e047f' target=_blank style='color:#2F92EE;'>#CID#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=29, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=4704, encryptionId=f88a4e047f, topicName=CID)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=570f404, createdName=xlxchina, createdTime=Sat Apr 18 10:53:00 CST 2020, time=2020-04-18, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1553356, encodeId=a5c7155335609, content=<a href='/topic/show?id=81bd923e559' target=_blank style='color:#2F92EE;'>#质谱#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=29, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=92375, encryptionId=81bd923e559, topicName=质谱)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=96fd14366106, createdName=jeanqiuqiu, createdTime=Mon Aug 05 13:53:00 CST 2019, time=2019-08-05, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1609904, encodeId=74071609904e5, content=<a href='/topic/show?id=eb6b1145939' target=_blank style='color:#2F92EE;'>#Med#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=25, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=11459, encryptionId=eb6b1145939, topicName=Med)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=552a19396740, createdName=ms3994565386320060, createdTime=Mon Aug 05 13:53:00 CST 2019, time=2019-08-05, status=1, ipAttribution=)]
    2019-08-05 ms3994565386320060

    #Med#

    0