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Carbohydr Polym：可注射的壳聚糖-羟基磷灰石水凝胶促进间充质干细胞的成骨分化

2018-08-05 MedSci MedSci原创

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可注射水凝胶已成为用于组织工程应用的一种有前途的生物材料。本研究旨在评估pH响应性壳聚糖-羟基磷灰石水凝胶作为包封的间充质干细胞（MSCs）成骨分化的三维支持的潜力。 在培养28天期间，在模拟生理条件下，水凝胶的体外酶促降解的特征在于溶胀测量，分子量测定和水凝胶微观结构的SEM分析。通过成骨Runx2，I型胶原和骨钙素免疫染色和碱性磷酸酶定量确认包封的MSC的成骨分化。通过茜素红和von

可注射水凝胶已成为用于组织工程应用的一种有前途的生物材料。本研究旨在评估pH响应性壳聚糖-羟基磷灰石水凝胶作为包封的间充质干细胞（MSCs）成骨分化的三维支持的潜力。

在培养28天期间，在模拟生理条件下，水凝胶的体外酶促降解的特征在于溶胀测量，分子量测定和水凝胶微观结构的SEM分析。通过成骨Runx2，I型胶原和骨钙素免疫染色和碱性磷酸酶定量确认包封的MSC的成骨分化。通过茜素红和von Kossa染色检测到的晚期成骨标志物（磷酸钙）的沉积表明细胞外基质矿化。

总之，该研究结果表明，研究所制备的pH响应性壳聚糖-羟基磷灰石水凝胶可作为促进MSCs成骨分化的三维支架。

原始出处：

Ressler A, Ródenas-Rochina J, et al., Injectable chitosan-hydroxyapatite hydrogels promote the osteogenic differentiation of mesenchymal stem cells. Carbohydr Polym. 2018 Oct 1;197:469-477. doi: 10.1016/j.carbpol.2018.06.029. Epub 2018 Jun 6.

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    2018-10-25 HinsMax

    #骨分化#

    0

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    2019-03-01 h.y200607_23674418

    #间充质干细#

    0

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    2018-08-12 衣带渐宽

    学习

    0

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createdAvatar=null, createdBy=e09e6383467, createdName=lishizhe, createdTime=Tue Aug 07 06:20:00 CST 2018, time=2018-08-07, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1582414, encodeId=eed3158241415, content=<a href='/topic/show?id=bda942398cd' target=_blank style='color:#2F92EE;'>#壳聚糖#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=30, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=42398, encryptionId=bda942398cd, topicName=壳聚糖)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=76f716920725, createdName=dlwang_26703132, createdTime=Tue Aug 07 06:20:00 CST 2018, time=2018-08-07, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1622130, encodeId=833716221307b, content=<a href='/topic/show?id=f41b8000867' target=_blank style='color:#2F92EE;'>#羟基磷灰石#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=40, replyNumber=0, topicName=null, 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    2018-08-07 tastas

    #水凝胶#

    0

  5. 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topicId=null, topicList=[TopicDto(id=80008, encryptionId=f41b8000867, topicName=羟基磷灰石)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=395f25, createdName=爆笑小医, createdTime=Tue Aug 07 06:20:00 CST 2018, time=2018-08-07, status=1, ipAttribution=)]
    2018-08-07 ms5880526374914242

    #成骨分化#

    0

  6. 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time=2018-08-07, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1351783, encodeId=5ac81351e83ba, content=<a href='/topic/show?id=e95954093cd' target=_blank style='color:#2F92EE;'>#成骨#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=31, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=54093, encryptionId=e95954093cd, topicName=成骨)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=4c37265, createdName=zhaojie88, createdTime=Tue Aug 07 06:20:00 CST 2018, time=2018-08-07, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1460977, encodeId=0d2414609e7db, content=<a href='/topic/show?id=de4331310a4' target=_blank style='color:#2F92EE;'>#凝胶#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=27, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=31310, encryptionId=de4331310a4, topicName=凝胶)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=e09e6383467, createdName=lishizhe, createdTime=Tue Aug 07 06:20:00 CST 2018, time=2018-08-07, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1582414, encodeId=eed3158241415, content=<a href='/topic/show?id=bda942398cd' target=_blank style='color:#2F92EE;'>#壳聚糖#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=30, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=42398, encryptionId=bda942398cd, topicName=壳聚糖)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=76f716920725, createdName=dlwang_26703132, createdTime=Tue Aug 07 06:20:00 CST 2018, time=2018-08-07, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1622130, encodeId=833716221307b, content=<a href='/topic/show?id=f41b8000867' target=_blank style='color:#2F92EE;'>#羟基磷灰石#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=40, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=80008, encryptionId=f41b8000867, topicName=羟基磷灰石)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=395f25, createdName=爆笑小医, createdTime=Tue Aug 07 06:20:00 CST 2018, time=2018-08-07, status=1, ipAttribution=)]
    2018-08-07 zhaojie88

    #成骨#

    0

  7. 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time=2018-08-07, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1351783, encodeId=5ac81351e83ba, content=<a href='/topic/show?id=e95954093cd' target=_blank style='color:#2F92EE;'>#成骨#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=31, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=54093, encryptionId=e95954093cd, topicName=成骨)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=4c37265, createdName=zhaojie88, createdTime=Tue Aug 07 06:20:00 CST 2018, time=2018-08-07, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1460977, encodeId=0d2414609e7db, content=<a href='/topic/show?id=de4331310a4' target=_blank style='color:#2F92EE;'>#凝胶#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=27, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=31310, encryptionId=de4331310a4, topicName=凝胶)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=e09e6383467, createdName=lishizhe, createdTime=Tue Aug 07 06:20:00 CST 2018, time=2018-08-07, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1582414, encodeId=eed3158241415, content=<a href='/topic/show?id=bda942398cd' target=_blank style='color:#2F92EE;'>#壳聚糖#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=30, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=42398, encryptionId=bda942398cd, topicName=壳聚糖)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=76f716920725, createdName=dlwang_26703132, createdTime=Tue Aug 07 06:20:00 CST 2018, time=2018-08-07, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1622130, encodeId=833716221307b, content=<a href='/topic/show?id=f41b8000867' target=_blank style='color:#2F92EE;'>#羟基磷灰石#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=40, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=80008, encryptionId=f41b8000867, topicName=羟基磷灰石)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=395f25, createdName=爆笑小医, createdTime=Tue Aug 07 06:20:00 CST 2018, time=2018-08-07, status=1, ipAttribution=)]
    2018-08-07 lishizhe

    #凝胶#

    0

  8. 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createdAvatar=null, createdBy=e09e6383467, createdName=lishizhe, createdTime=Tue Aug 07 06:20:00 CST 2018, time=2018-08-07, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1582414, encodeId=eed3158241415, content=<a href='/topic/show?id=bda942398cd' target=_blank style='color:#2F92EE;'>#壳聚糖#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=30, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=42398, encryptionId=bda942398cd, topicName=壳聚糖)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=76f716920725, createdName=dlwang_26703132, createdTime=Tue Aug 07 06:20:00 CST 2018, time=2018-08-07, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1622130, encodeId=833716221307b, content=<a href='/topic/show?id=f41b8000867' target=_blank style='color:#2F92EE;'>#羟基磷灰石#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=40, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=80008, encryptionId=f41b8000867, topicName=羟基磷灰石)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=395f25, createdName=爆笑小医, createdTime=Tue Aug 07 06:20:00 CST 2018, time=2018-08-07, status=1, ipAttribution=)]
    2018-08-07 dlwang_26703132

    #壳聚糖#

    0

  9. 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topicId=null, topicList=[TopicDto(id=80008, encryptionId=f41b8000867, topicName=羟基磷灰石)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=395f25, createdName=爆笑小医, createdTime=Tue Aug 07 06:20:00 CST 2018, time=2018-08-07, status=1, ipAttribution=)]
    2018-08-07 爆笑小医

    #羟基磷灰石#

    0