Nephrol：葡萄籽原花青素提取物可预防CsA肾病
2012-12-26 Nephrology CMT 银子 编译
研究证实：葡萄籽原花青素提取物（Grape seed proanthocyanidin extract，GSPE）通过抗凋亡、抗氧化的作用预防CsA 肾病，能够在不影响环孢素A（cyclosporine，CsA）血浆浓度的情况下有效保护肾功能。因此，移植患者使用CsA预防移植物抗宿主反应（graft versus&n
研究证实：葡萄籽原花青素提取物（Grape seed proanthocyanidin extract，GSPE）通过抗凋亡、抗氧化的作用预防CsA肾病，能够在不影响环孢素A（cyclosporine，CsA）血浆浓度的情况下有效保护肾功能。因此，移植患者使用CsA预防移植物抗宿主反应（graft versus host disease，GVHD）时，加用GSPE将有助于预防CsA肾病。
Aim
Although the pathogenesis of cyclosporine (CsA) nephropathy is not completely understood, it is attributed to oxidative damage and apoptosis. Grape seed proanthocyanidin extract (GSPE) is a molecule with anti-oxidant and anti-apoptotic properties. Our aim was to demonstrate the effects of GSPE in preventing CsA nephropathy.
Methods
Twenty-four Sprague–Dawley rats were divided into four groups. The control, GSPE, CsA and CsA+GSPE groups were given 1 mL olive oil, 100 mg/kg GSPE, 25 mg/kg CsA and 100 mg/kg GSPE+25 mg/kg CsA, respectively. On day 21, blood samples were taken for blood urea nitrogen (BUN), creatinine and CsA levels, and renal tissue was used for total oxidant system (TOS), total anti-oxidant system (TAS), oxidative stress index (OSI) and malondialdehyde (MDA) measurements. In addition to renal histopathology, apoptosis staining was performed on renal tissue.
Results
The BUN, creatinine, TOS, OSI, MDA, histopathological score, and apoptotic index exhibited increases in the CsA group. In the CsA+GSPE group, however, BUN, creatinine, OSI, MDA, renal histopathological score and apoptotic index (AI) decreased and TAS levels increased. In addition, there was no difference between the CsA and CsA+GSPE groups with regard to CsA levels.
Conclusion
We demonstrated that GSPE prevents CsA nephropathy and that this effect is achieved by anti-apoptotic and anti-oxidant activity. We also achieved a significant recovery in kidney functions without affecting CsA plasma levels.
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