J Cell Biochem:胶原蛋白-藻酸盐-纳米二氧化硅微球改善了人类成骨细胞样MG-63细胞的成骨潜能

2019-04-28 不详 网络

模块化骨组织工程是一种替代受损骨组织的替代方法。水凝胶微胶囊可通过提供3D条件和增加的细胞间相互作用来促进治疗性质。我们研究了富含胶原蛋白和明胶的藻酸盐-纳米二氧化硅水凝胶对人类成骨细胞样MG-63细胞的成骨特性。对于细胞包封,将细胞分成三组;对照(藻酸盐+纳米二氧化硅),胶原蛋白(藻酸盐+胶原蛋白+纳米二氧化硅)和明胶(藻酸盐+明胶+纳米二氧化硅)并膨胀28天。通过台盼蓝染色和3-(4,5-二甲

模块化骨组织工程是一种替代受损骨组织的替代方法。水凝胶微胶囊可通过提供3D条件和增加的细胞间相互作用来促进治疗性质。我们研究了富含胶原蛋白和明胶的藻酸盐-纳米二氧化硅水凝胶对人类成骨细胞样MG-63细胞的成骨特性。

对于细胞包封,将细胞分成三组;对照(藻酸盐+纳米二氧化硅),胶原蛋白(藻酸盐+胶原蛋白+纳米二氧化硅)和明胶(藻酸盐+明胶+纳米二氧化硅)并膨胀28天。通过台盼蓝染色和3-(4,5-二甲基噻唑-2-基)-2,5-二苯基溴化四唑(MTT)测定法测定细胞活力。为了确认成骨潜能,我们测量了碱性磷酸酶活性。茜素红S染色用于分析羟基磷灰石的表达,并通过实时聚合酶链反应评估转录BMP-2,骨钙蛋白和骨粘连蛋白。

结果显示,与对照组和明胶组相比,胶原蛋白底物导致溶胀率降低(P <0.05)。与其他组相比,胶原蛋白具有提高机械强度和产生多孔膜结构的潜力。与对照相比,添加胶原蛋白(4倍)和明胶(1.5倍)增加了细胞增殖率(P <0.05)。生化分析和茜素红S染色显示,通过诱导碱性磷酸酶和基质矿化,胶原诱导成骨。来自胶原蛋白组的细胞中骨钙蛋白和BMP-2的表达增加。

总之,该研究结果表明,天然聚合物胶原蛋白和明胶与藻酸盐+纳米二氧化硅的组合可以增加人成骨细胞的成骨潜力。

原始出处:

Khatami N, Khoshfetrat AB, et al., Collagen-alginate-nano-silica microspheres improved the osteogenic potential of human osteoblast-like MG-63 cells. J Cell Biochem. 2019 Apr 24. doi: 10.1002/jcb.28768. 

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    2019-07-01 维他命
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    2019-07-08 sunylz
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