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Nanomedicine：外泌体的粒子分布影响细胞对外泌体的摄取

2016-12-23 土豆 外泌体之家 土豆 外泌体之家

细胞对外泌体
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外泌体可以特异性激发一些细胞反应，表明它们可以在作为治疗递送载体方面有巨大的潜力。外泌体的囊泡性质使得它们适合作为用于药物或核酸递送的潜在纳米载体。在这里，研究人员需要解决的问题是，富集外泌体的的不同制备方法是否可以影响它们的细胞吸收和他们的触发细胞反应的能力。12月16日，来自意大利乌迪内大学的研究人员利用从高分级胶质瘤患者分离的胶质瘤相关干细胞的上清液，比较了超高速离心和基于聚合物沉淀方法。研

外泌体可以特异性激发一些细胞反应，表明它们可以在作为治疗递送载体方面有巨大的潜力。外泌体的囊泡性质使得它们适合作为用于药物或核酸递送的潜在纳米载体。在这里，研究人员需要解决的问题是，富集外泌体的的不同制备方法是否可以影响它们的细胞吸收和他们的触发细胞反应的能力。12月16日，来自意大利乌迪内大学的研究人员利用从高分级胶质瘤患者分离的胶质瘤相关干细胞的上清液，比较了超高速离心和基于聚合物沉淀方法。研究人员确定了纯化后的粒度分布，及其与培养的胶质母细胞瘤细胞的摄取、增殖和迁移的相关性。研究结果表明，基于聚合物的沉淀表现出更小的粒度分布、更快的目标细胞摄取和增加细胞迁移能力。分离方法依赖的粒子群对细胞迁移性的不同影响表明，它们的大小分布也会影响外泌体的治疗潜力。

图1：利用聚合物沉淀分离的外泌体比超离法的粒子分布要小。

图2：划痕实验证实聚合物沉淀所得的小粒径的外泌体被细胞摄取后迁移更快。

原始出处：

Caponnetto F, Manini I, Skrap M, Palmai-Pallag T, Di Loreto C, Beltrami AP,Cesselli D, Ferrari E. Size-dependent cellular uptake of exosomes. Nanomedicine. 2016 Dec 16.  PMID: 27993726.

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    2017-01-14 jeanqiuqiu

    #DIC#

    0

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    2017-05-12 ms1292405679162969

    #细胞对外泌体#

    0

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    2017-09-04 欢乐的树博士

    赞一个.学习了!

    0

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    2017-12-05 kalseyzl

    #CIN#

    0

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    2016-12-25 1e10c84am36(暂无匿称)

    文章好好

    0

  6. [GetPortalCommentsPageByObjectIdResponse(id=1858706, encodeId=15391858e06f1, content=<a href='/topic/show?id=e0a9593596' target=_blank style='color:#2F92EE;'>#DIC#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=23, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=5935, encryptionId=e0a9593596, topicName=DIC)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=6f7770, createdName=jeanqiuqiu, createdTime=Sat Jan 14 08:26:00 CST 2017, time=2017-01-14, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1992778, encodeId=c6081992e789c, content=<a href='/topic/show?id=0f0fe789071' target=_blank style='color:#2F92EE;'>#细胞对外泌体#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=25, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=77890, encryptionId=0f0fe789071, topicName=细胞对外泌体)], attachment=null, authenticateStatus=null, createdAvatar=http://thirdqq.qlogo.cn/qqapp/101296147/BF4BC9769C2FF12AC93CAA0069C0696C/100, createdBy=7bb22500091, createdName=ms1292405679162969, createdTime=Fri May 12 07:26:00 CST 2017, time=2017-05-12, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=240415, encodeId=ac3b240415e1, content=赞一个.学习了!, beContent=null, objectType=article, channel=null, level=null, likeNumber=64, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=http://login.medsci.cn/upload/avatar/8ab47519872135abb0a73648d5c28db5/e26e9c24b8c138494e359b288b9601d9.jpg, createdBy=96e82024468, createdName=欢乐的树博士, createdTime=Mon Sep 04 16:45:44 CST 2017, time=2017-09-04, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1773579, encodeId=b0191e73579d1, content=<a href='/topic/show?id=5f014e1852' target=_blank style='color:#2F92EE;'>#CIN#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=28, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=4718, encryptionId=5f014e1852, topicName=CIN)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=a9a038523631, createdName=kalseyzl, createdTime=Tue Dec 05 02:26:00 CST 2017, time=2017-12-05, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=165546, encodeId=554e165546df, content=文章好好, beContent=null, objectType=article, channel=null, level=null, likeNumber=57, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/jW482SpianMayicTRbRZ5RzdAIB7UwTkHt6fQkBP8ictliaXtc5gCUmYdAW1sAETC2nhO3q6YjRHkUibyOCtP8ibmXNObTyd63A076/0, createdBy=d4111948983, createdName=1e10c84am36(暂无匿称), createdTime=Sun Dec 25 10:54:51 CST 2016, time=2016-12-25, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1577492, encodeId=cc5e15e74922f, content=<a href='/topic/show?id=cda91148004' target=_blank style='color:#2F92EE;'>#Medicine#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=22, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=11480, encryptionId=cda91148004, topicName=Medicine)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=54cd16396700, createdName=ms1692212821629096, createdTime=Sun Dec 25 00:26:00 CST 2016, time=2016-12-25, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1607228, encodeId=c856160e22878, content=<a href='/topic/show?id=eb6b1145939' target=_blank style='color:#2F92EE;'>#Med#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=21, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=11459, encryptionId=eb6b1145939, topicName=Med)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=552a19396740, createdName=ms3994565386320060, createdTime=Sun Dec 25 00:26:00 CST 2016, time=2016-12-25, status=1, ipAttribution=)]
    2016-12-25 ms1692212821629096

    #Medicine#

    0

  7. [GetPortalCommentsPageByObjectIdResponse(id=1858706, encodeId=15391858e06f1, content=<a href='/topic/show?id=e0a9593596' target=_blank style='color:#2F92EE;'>#DIC#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=23, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=5935, encryptionId=e0a9593596, topicName=DIC)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=6f7770, createdName=jeanqiuqiu, createdTime=Sat Jan 14 08:26:00 CST 2017, time=2017-01-14, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1992778, encodeId=c6081992e789c, content=<a href='/topic/show?id=0f0fe789071' target=_blank style='color:#2F92EE;'>#细胞对外泌体#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=25, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=77890, encryptionId=0f0fe789071, topicName=细胞对外泌体)], attachment=null, authenticateStatus=null, createdAvatar=http://thirdqq.qlogo.cn/qqapp/101296147/BF4BC9769C2FF12AC93CAA0069C0696C/100, createdBy=7bb22500091, createdName=ms1292405679162969, createdTime=Fri May 12 07:26:00 CST 2017, time=2017-05-12, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=240415, encodeId=ac3b240415e1, content=赞一个.学习了!, beContent=null, objectType=article, channel=null, level=null, likeNumber=64, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=http://login.medsci.cn/upload/avatar/8ab47519872135abb0a73648d5c28db5/e26e9c24b8c138494e359b288b9601d9.jpg, createdBy=96e82024468, createdName=欢乐的树博士, createdTime=Mon Sep 04 16:45:44 CST 2017, time=2017-09-04, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1773579, encodeId=b0191e73579d1, content=<a href='/topic/show?id=5f014e1852' target=_blank style='color:#2F92EE;'>#CIN#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=28, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=4718, encryptionId=5f014e1852, topicName=CIN)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=a9a038523631, createdName=kalseyzl, createdTime=Tue Dec 05 02:26:00 CST 2017, time=2017-12-05, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=165546, encodeId=554e165546df, content=文章好好, beContent=null, objectType=article, channel=null, level=null, likeNumber=57, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/jW482SpianMayicTRbRZ5RzdAIB7UwTkHt6fQkBP8ictliaXtc5gCUmYdAW1sAETC2nhO3q6YjRHkUibyOCtP8ibmXNObTyd63A076/0, createdBy=d4111948983, createdName=1e10c84am36(暂无匿称), createdTime=Sun Dec 25 10:54:51 CST 2016, time=2016-12-25, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1577492, encodeId=cc5e15e74922f, content=<a href='/topic/show?id=cda91148004' target=_blank style='color:#2F92EE;'>#Medicine#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=22, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=11480, encryptionId=cda91148004, topicName=Medicine)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=54cd16396700, createdName=ms1692212821629096, createdTime=Sun Dec 25 00:26:00 CST 2016, time=2016-12-25, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1607228, encodeId=c856160e22878, content=<a href='/topic/show?id=eb6b1145939' target=_blank style='color:#2F92EE;'>#Med#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=21, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=11459, encryptionId=eb6b1145939, topicName=Med)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=552a19396740, createdName=ms3994565386320060, createdTime=Sun Dec 25 00:26:00 CST 2016, time=2016-12-25, status=1, ipAttribution=)]
    2016-12-25 ms3994565386320060

    #Med#

    0