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CLIN CHEM LAB MED：粪便标本长期冷冻及解冻对粪便血红蛋白浓度及粪便免疫化学检测诊断效能的影响

2020-03-21 MedSci原创 MedSci原创

血红蛋白浓度；免疫化学检测
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历年收集和冷冻的粪便样本是对粪便免疫化学试验(FITs) 开展有效回顾性评价的重要资源。本研究的目的是评估长时间的冷冻储存和冻融循环是否会影响粪便血红蛋白(Hb)的测量和对FITs诊断的能力。

历年收集和冷冻的粪便样本是对粪便免疫化学试验(FITs) 开展有效回顾性评价的重要资源。本研究的目的是评估长时间的冷冻储存和冻融循环是否会影响粪便血红蛋白(Hb)的测量和对FITs诊断的能力。

2005年到2010年，筛查结肠镜检查(n = 2042)和临床结直肠癌(CRC)病例(n = 184)的参与者提供了 (60毫升) 粪便样本。在- 80°C下，样品保存11年，且经历了3次冻融循环。在每个周期之间，使用制造商的一个或两个适合的采样装置(RIDASCREEN, OC-Sensor)提取一定数量的粪便。计算和比较了不同冻融周期的粪便Hb浓度和诊断性能。

研究结果显示，对于RIDASCREEN和OC-Sensor，分别对504名和551名研究参与者进行了重复测量。Hb浓度相关性强(分别为0.77和0.85)，在相同的拟合重复测量中，诊断性能指标相似。对于RIDASCREEN，我们发现在第三个循环后，Hb水平、敏感性和曲线下面积(auc)比第一个冻融循环后还要稍高。对于OC-Sensor，在长时间的储存和一个额外的冻融循环后，Hb的水平、敏感性和AUCs稍低。

研究结果表明，即使经过长期的冷冻储存和多次的冻融循环，血红蛋白和诊断性能的测量结果也相当稳定。在前瞻性筛选研究中收集的粪便样本，在分析前冷冻在- 80°C，有助于及时有效地回顾评估拟合效果。

原始出处：

Anton Gies，Tobias Niedermaier，Effect of long-term frozen storage and thawing of stool samples on faecal haemoglobin concentration and diagnostic performance of faecal immunochemical tests

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    2020-12-09 jklm09

    #粪便标本#

    0

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    2020-06-30 ms306040998833292

    #标本#

    0

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    2020-03-23 huangdf

    #便血#

    0

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    2020-03-23 Homburg

    #粪便#

    0

  5. [GetPortalCommentsPageByObjectIdResponse(id=1804205, encodeId=3d19180420576, content=<a href='/topic/show?id=9931e6317f6' target=_blank style='color:#2F92EE;'>#粪便标本#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=30, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=76317, encryptionId=9931e6317f6, topicName=粪便标本)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=44a360, createdName=jklm09, createdTime=Wed Dec 09 04:59:51 CST 2020, time=2020-12-09, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1758975, encodeId=9a391e5897549, content=<a href='/topic/show?id=39e96195411' target=_blank style='color:#2F92EE;'>#标本#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=28, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=61954, encryptionId=39e96195411, topicName=标本)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=45f437392611, createdName=ms306040998833292, createdTime=Tue Jun 30 03:59:51 CST 2020, time=2020-06-30, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1371940, encodeId=165013e1940d4, content=<a href='/topic/show?id=815e2e060ce' target=_blank style='color:#2F92EE;'>#便血#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=45, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=27060, encryptionId=815e2e060ce, topicName=便血)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=e2e0330, createdName=huangdf, createdTime=Mon Mar 23 12:59:51 CST 2020, time=2020-03-23, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1466907, encodeId=1e94146690ebb, content=<a href='/topic/show?id=edd5e630426' target=_blank style='color:#2F92EE;'>#粪便#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=29, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=76304, encryptionId=edd5e630426, topicName=粪便)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=03cc6863823, createdName=Homburg, createdTime=Mon Mar 23 12:59:51 CST 2020, time=2020-03-23, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1476293, encodeId=4c4d14e629378, content=<a href='/topic/show?id=7223e63103e' target=_blank style='color:#2F92EE;'>#粪便免疫化学检测#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=38, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=76310, encryptionId=7223e63103e, topicName=粪便免疫化学检测)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=42c67269942, createdName=wenzhiren, createdTime=Mon Mar 23 12:59:51 CST 2020, time=2020-03-23, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1544635, encodeId=df6f1544635b6, content=<a href='/topic/show?id=ac3d6549e75' target=_blank style='color:#2F92EE;'>#浓度#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=23, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=65497, encryptionId=ac3d6549e75, topicName=浓度)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=16b213663040, createdName=12498974m55暂无昵称, createdTime=Mon Mar 23 12:59:51 CST 2020, time=2020-03-23, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1557158, encodeId=fa17155e158c5, content=<a href='/topic/show?id=8e2ae632643' target=_blank style='color:#2F92EE;'>#粪便血红蛋白#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=25, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=76326, encryptionId=8e2ae632643, topicName=粪便血红蛋白)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=d90d14863929, createdName=psybestwish, createdTime=Mon Mar 23 12:59:51 CST 2020, time=2020-03-23, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1610379, encodeId=878c16103e94d, content=<a href='/topic/show?id=eb6b1145939' target=_blank style='color:#2F92EE;'>#Med#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=16, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=11459, encryptionId=eb6b1145939, topicName=Med)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=552a19396740, createdName=ms3994565386320060, createdTime=Mon Mar 23 12:59:51 CST 2020, time=2020-03-23, status=1, ipAttribution=)]
    2020-03-23 wenzhiren

    #粪便免疫化学检测#

    0

  6. [GetPortalCommentsPageByObjectIdResponse(id=1804205, encodeId=3d19180420576, content=<a href='/topic/show?id=9931e6317f6' target=_blank style='color:#2F92EE;'>#粪便标本#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=30, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=76317, encryptionId=9931e6317f6, topicName=粪便标本)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=44a360, createdName=jklm09, createdTime=Wed Dec 09 04:59:51 CST 2020, time=2020-12-09, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1758975, encodeId=9a391e5897549, content=<a href='/topic/show?id=39e96195411' target=_blank style='color:#2F92EE;'>#标本#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=28, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=61954, encryptionId=39e96195411, topicName=标本)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=45f437392611, createdName=ms306040998833292, createdTime=Tue Jun 30 03:59:51 CST 2020, time=2020-06-30, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1371940, encodeId=165013e1940d4, content=<a href='/topic/show?id=815e2e060ce' target=_blank style='color:#2F92EE;'>#便血#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=45, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=27060, encryptionId=815e2e060ce, topicName=便血)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=e2e0330, createdName=huangdf, createdTime=Mon Mar 23 12:59:51 CST 2020, time=2020-03-23, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1466907, encodeId=1e94146690ebb, content=<a href='/topic/show?id=edd5e630426' target=_blank style='color:#2F92EE;'>#粪便#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=29, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=76304, encryptionId=edd5e630426, topicName=粪便)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=03cc6863823, createdName=Homburg, createdTime=Mon Mar 23 12:59:51 CST 2020, time=2020-03-23, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1476293, encodeId=4c4d14e629378, content=<a href='/topic/show?id=7223e63103e' target=_blank style='color:#2F92EE;'>#粪便免疫化学检测#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=38, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=76310, encryptionId=7223e63103e, topicName=粪便免疫化学检测)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=42c67269942, createdName=wenzhiren, createdTime=Mon Mar 23 12:59:51 CST 2020, time=2020-03-23, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1544635, encodeId=df6f1544635b6, content=<a href='/topic/show?id=ac3d6549e75' target=_blank style='color:#2F92EE;'>#浓度#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=23, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=65497, encryptionId=ac3d6549e75, topicName=浓度)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=16b213663040, createdName=12498974m55暂无昵称, createdTime=Mon Mar 23 12:59:51 CST 2020, time=2020-03-23, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1557158, encodeId=fa17155e158c5, content=<a href='/topic/show?id=8e2ae632643' target=_blank style='color:#2F92EE;'>#粪便血红蛋白#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=25, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=76326, encryptionId=8e2ae632643, topicName=粪便血红蛋白)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=d90d14863929, createdName=psybestwish, createdTime=Mon Mar 23 12:59:51 CST 2020, time=2020-03-23, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1610379, encodeId=878c16103e94d, content=<a href='/topic/show?id=eb6b1145939' target=_blank style='color:#2F92EE;'>#Med#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=16, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=11459, encryptionId=eb6b1145939, topicName=Med)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=552a19396740, createdName=ms3994565386320060, createdTime=Mon Mar 23 12:59:51 CST 2020, time=2020-03-23, status=1, ipAttribution=)]
    2020-03-23 12498974m55暂无昵称

    #浓度#

    0

  7. [GetPortalCommentsPageByObjectIdResponse(id=1804205, encodeId=3d19180420576, content=<a href='/topic/show?id=9931e6317f6' target=_blank style='color:#2F92EE;'>#粪便标本#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=30, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=76317, encryptionId=9931e6317f6, topicName=粪便标本)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=44a360, createdName=jklm09, createdTime=Wed Dec 09 04:59:51 CST 2020, time=2020-12-09, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1758975, encodeId=9a391e5897549, content=<a href='/topic/show?id=39e96195411' target=_blank style='color:#2F92EE;'>#标本#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=28, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=61954, encryptionId=39e96195411, topicName=标本)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=45f437392611, createdName=ms306040998833292, createdTime=Tue Jun 30 03:59:51 CST 2020, time=2020-06-30, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1371940, encodeId=165013e1940d4, content=<a href='/topic/show?id=815e2e060ce' target=_blank style='color:#2F92EE;'>#便血#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=45, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=27060, encryptionId=815e2e060ce, topicName=便血)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=e2e0330, createdName=huangdf, createdTime=Mon Mar 23 12:59:51 CST 2020, time=2020-03-23, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1466907, encodeId=1e94146690ebb, content=<a href='/topic/show?id=edd5e630426' target=_blank style='color:#2F92EE;'>#粪便#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=29, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=76304, encryptionId=edd5e630426, topicName=粪便)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=03cc6863823, createdName=Homburg, createdTime=Mon Mar 23 12:59:51 CST 2020, time=2020-03-23, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1476293, encodeId=4c4d14e629378, content=<a href='/topic/show?id=7223e63103e' target=_blank style='color:#2F92EE;'>#粪便免疫化学检测#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=38, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=76310, encryptionId=7223e63103e, topicName=粪便免疫化学检测)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=42c67269942, createdName=wenzhiren, createdTime=Mon Mar 23 12:59:51 CST 2020, time=2020-03-23, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1544635, encodeId=df6f1544635b6, content=<a href='/topic/show?id=ac3d6549e75' target=_blank style='color:#2F92EE;'>#浓度#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=23, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=65497, encryptionId=ac3d6549e75, topicName=浓度)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=16b213663040, createdName=12498974m55暂无昵称, createdTime=Mon Mar 23 12:59:51 CST 2020, time=2020-03-23, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1557158, encodeId=fa17155e158c5, content=<a href='/topic/show?id=8e2ae632643' target=_blank style='color:#2F92EE;'>#粪便血红蛋白#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=25, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=76326, encryptionId=8e2ae632643, topicName=粪便血红蛋白)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=d90d14863929, createdName=psybestwish, createdTime=Mon Mar 23 12:59:51 CST 2020, time=2020-03-23, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1610379, encodeId=878c16103e94d, content=<a href='/topic/show?id=eb6b1145939' target=_blank style='color:#2F92EE;'>#Med#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=16, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=11459, encryptionId=eb6b1145939, topicName=Med)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=552a19396740, createdName=ms3994565386320060, createdTime=Mon Mar 23 12:59:51 CST 2020, time=2020-03-23, status=1, ipAttribution=)]
    2020-03-23 psybestwish

    #粪便血红蛋白#

    0

  8. [GetPortalCommentsPageByObjectIdResponse(id=1804205, encodeId=3d19180420576, content=<a href='/topic/show?id=9931e6317f6' target=_blank style='color:#2F92EE;'>#粪便标本#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=30, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=76317, encryptionId=9931e6317f6, topicName=粪便标本)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=44a360, createdName=jklm09, createdTime=Wed Dec 09 04:59:51 CST 2020, time=2020-12-09, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1758975, encodeId=9a391e5897549, content=<a href='/topic/show?id=39e96195411' target=_blank style='color:#2F92EE;'>#标本#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=28, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=61954, encryptionId=39e96195411, topicName=标本)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=45f437392611, createdName=ms306040998833292, createdTime=Tue Jun 30 03:59:51 CST 2020, time=2020-06-30, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1371940, encodeId=165013e1940d4, content=<a href='/topic/show?id=815e2e060ce' target=_blank style='color:#2F92EE;'>#便血#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=45, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=27060, encryptionId=815e2e060ce, topicName=便血)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=e2e0330, createdName=huangdf, createdTime=Mon Mar 23 12:59:51 CST 2020, time=2020-03-23, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1466907, encodeId=1e94146690ebb, content=<a href='/topic/show?id=edd5e630426' target=_blank style='color:#2F92EE;'>#粪便#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=29, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=76304, encryptionId=edd5e630426, topicName=粪便)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=03cc6863823, createdName=Homburg, createdTime=Mon Mar 23 12:59:51 CST 2020, time=2020-03-23, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1476293, encodeId=4c4d14e629378, content=<a href='/topic/show?id=7223e63103e' target=_blank style='color:#2F92EE;'>#粪便免疫化学检测#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=38, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=76310, encryptionId=7223e63103e, topicName=粪便免疫化学检测)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=42c67269942, createdName=wenzhiren, createdTime=Mon Mar 23 12:59:51 CST 2020, time=2020-03-23, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1544635, encodeId=df6f1544635b6, content=<a href='/topic/show?id=ac3d6549e75' target=_blank style='color:#2F92EE;'>#浓度#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=23, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=65497, encryptionId=ac3d6549e75, topicName=浓度)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=16b213663040, createdName=12498974m55暂无昵称, createdTime=Mon Mar 23 12:59:51 CST 2020, time=2020-03-23, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1557158, encodeId=fa17155e158c5, content=<a href='/topic/show?id=8e2ae632643' target=_blank style='color:#2F92EE;'>#粪便血红蛋白#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=25, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=76326, encryptionId=8e2ae632643, topicName=粪便血红蛋白)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=d90d14863929, createdName=psybestwish, createdTime=Mon Mar 23 12:59:51 CST 2020, time=2020-03-23, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1610379, encodeId=878c16103e94d, content=<a href='/topic/show?id=eb6b1145939' target=_blank style='color:#2F92EE;'>#Med#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=16, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=11459, encryptionId=eb6b1145939, topicName=Med)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=552a19396740, createdName=ms3994565386320060, createdTime=Mon Mar 23 12:59:51 CST 2020, time=2020-03-23, status=1, ipAttribution=)]
    2020-03-23 ms3994565386320060

    #Med#

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