JCLA:快速鉴定不动杆菌属细菌的方法

2017-05-27 MedSci MedSci原创

目前,不动杆菌属有50多种,其中四种亲缘关系很近,采用表型或基因型方法难以区分,如不动杆菌- 醋酸鲍曼不动杆菌复合体(ABC)。在流行病学方面,对不动杆菌属在种水平的正确识别是非常必要的。

近日,国际杂志Journal of Clinical Laboratory Analysis上在线发表一项关于基于gyrB基因的PCR检测方法快速鉴定鲍曼不动杆菌-醋酸钙不动杆菌的研究。

目前,不动杆菌属有50多种,其中四种亲缘关系很近,采用表型或基因型方法难以区分,如不动杆菌- 醋酸鲍曼不动杆菌复合体(ABC)。在流行病学方面,对不动杆菌属在种水平的正确识别是非常必要的。

本研究评估了利用gyrB基因的多重PCRITS 16S-23S片段的测序作为金标准来鉴定ABC的种类。研究纳入了2011年巴西南部三家医院鉴定为为醋酸鲍曼不动杆菌的分离株。

研究共获得了117份分离株,通过PCR检测gyrB基因,其中106份(90.6%)经确认为鲍曼不动杆菌,6份(5.1%)为A. nosocomialis4份(3.4%)为A. pittii。只有一个分离株没有显示gyrB基因的PCR产物;通过ITS的测序将该分离株鉴定为A. genospecie。同时,研究人员还能从呼吸道(8 / 72.7%),血液(2/18.2%)和尿(1/9.1%)中得到非鲍曼不动杆菌分离物,表明这些种类可引起严重感染

这些发现证明,gyrB的多重PCR是识别种级别的分离株的可行其简便的方法。

原始出处:

Aline B. Teixeira, Juliana Barin, et.al. PCR Assay Based on the gyrB Gene for Rapid Identification of Acinetobacter baumannii-calcoaceticus Complex at Specie Level.

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    2017-05-31 laoli

    谢谢分享,学习了!

    0

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    2017-05-29 130****4638

    学习了谢谢分享。

    0

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    2017-05-29 changjiu

    好文章,谢谢

    0

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    2017-05-28 laymankey

    感谢分享一下!!

    0

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    2017-05-28 天涯183

    非常好的文章,学习了,很受益

    0

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    2017-05-28 飛歌

    学习了很有用

    0

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    2017-05-28 杨利洪

    学习并分享了

    0

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    2017-05-27 有备才能无患

    这些发现证明,gyrB的多重PCR是识别种级别的分离株的可行其简便的方法。

    0