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CLIN CHEM：利用3种不同方法测量脂蛋白相关磷脂酶A2

2018-04-04 MedSci MedSci原创

脂蛋白相关磷脂酶A2
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脂蛋白相关磷脂酶A2（Lp-PLA 2）是一种与炎症相关的酶，被用作心血管疾病风险的生物标志物。已有研究表明Lp-PLA 2的浓度和活性使临床相关的。然而，通过标准的基于ELISA的免疫测定法测量的Lp-PLA 2的血清浓度与该酶的活性之间存在不一致，从而导致根据测定形式的风险分类的实质性存在一定问题。 研究人员开发了2种基于LC-MS / MS的检测方法来定量血清Lp-PLA 2活性（产物

脂蛋白相关磷脂酶A2（Lp-PLA 2）是一种与炎症相关的酶，被用作心血管疾病风险的生物标志物。已有研究表明Lp-PLA 2的浓度和活性使临床相关的。然而，通过标准的基于ELISA的免疫测定法测量的Lp-PLA 2的血清浓度与该酶的活性之间存在不一致，从而导致根据测定形式的风险分类的实质性存在一定问题。 研究人员开发了2种基于LC-MS / MS的检测方法来定量血清Lp-PLA 2活性（产物的多反应监测检测）和浓度[稳定同位素标准和抗肽抗体捕获（SISCAPA）免疫亲和]，并与商业的比色活性和免疫测定浓度测定相关性进行比较。通过使用免疫沉淀和蛋白质印迹分析来评估Lp-PLA 2与脂蛋白之间的关联以及所选去污剂释放Lp-PLA 2的效果。 结果显示通过定量SISCAPA-质谱法测量的血清Lp-PLA 2浓度显著高于通常通过免疫测定法测量的浓度，并显示与Lp-PLA 2活性改善。使用去污剂从脂蛋白复合物中释放Lp-PLA 2可显著增加通过免疫测定检测到的蛋白质的量，并且提高了与活性测量的一致性。 研究表明通过LC-MS / MS 分析对Lp-PLA 2浓度和活性进行定量分析，为解决Lp-P

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    2018-04-24 海桐

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  2. [GetPortalCommentsPageByObjectIdResponse(id=308883, encodeId=64943088839c, content=赞, beContent=null, objectType=article, channel=null, level=null, likeNumber=41, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=f04a1658586, createdName=海桐, createdTime=Tue Apr 24 16:54:25 CST 2018, time=2018-04-24, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1424703, encodeId=ad3d1424e034a, content=<a href='/topic/show?id=5a3b8441438' target=_blank style='color:#2F92EE;'>#脂蛋白#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=16, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=84414, encryptionId=5a3b8441438, topicName=脂蛋白)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=23ea3924919, createdName=juliusluan79, createdTime=Fri Apr 06 01:58:00 CST 2018, time=2018-04-06, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=302671, encodeId=f15e3026e1d6, content=阅, beContent=null, objectType=article, channel=null, level=null, likeNumber=43, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://cdnapi.center.medsci.cn/medsci/head/2017/09/07/a9772c74ffd6edc03a7f9af07b4747f6.jpg, createdBy=b3722138742, createdName=sunfeifeiyang, createdTime=Wed Apr 04 11:08:55 CST 2018, time=2018-04-04, status=1, ipAttribution=)]
    2018-04-06 juliusluan79

    #脂蛋白#

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  3. [GetPortalCommentsPageByObjectIdResponse(id=308883, encodeId=64943088839c, content=赞, beContent=null, objectType=article, channel=null, level=null, likeNumber=41, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=f04a1658586, createdName=海桐, createdTime=Tue Apr 24 16:54:25 CST 2018, time=2018-04-24, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1424703, encodeId=ad3d1424e034a, content=<a href='/topic/show?id=5a3b8441438' target=_blank style='color:#2F92EE;'>#脂蛋白#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=16, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=84414, encryptionId=5a3b8441438, topicName=脂蛋白)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=23ea3924919, createdName=juliusluan79, createdTime=Fri Apr 06 01:58:00 CST 2018, time=2018-04-06, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=302671, encodeId=f15e3026e1d6, content=阅, beContent=null, objectType=article, channel=null, level=null, likeNumber=43, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://cdnapi.center.medsci.cn/medsci/head/2017/09/07/a9772c74ffd6edc03a7f9af07b4747f6.jpg, createdBy=b3722138742, createdName=sunfeifeiyang, createdTime=Wed Apr 04 11:08:55 CST 2018, time=2018-04-04, status=1, ipAttribution=)]
    2018-04-04 sunfeifeiyang

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